巻号一覧
33 巻 (2012)
- 6 号 p. 323-
- 5 号 p. 255-
- 4 号 p. 201-
- 3 号 p. 145-
- 2 号 p. 67-
- 1 号 p. 1-
33 巻, 2 号
April
選択された号の論文の9件中1～9を表示しています
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Full papers
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Takayuki Yoshida, Ken Katsuya, Takahiro Oka, Shin-ichi Koizumi, Daiko ...2012 年 33 巻 2 号 p. 67-74
発行日: 2012年
公開日: 2012/04/26
ジャーナル フリーThe aryl hydrocarbon receptor (AhR) has been shown to play important roles in the immune system, and contributions of AhR ligands to the differentiation and functions of Th17/Treg cells have recently been established. However, it has not been fully clarified whether AhR plays roles in B cell differentiation and functions. The environmental pollutant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a highly potent AhR agonist, was reported to suppress the production of immunoglobulin M (IgM) in a transformed mouse B cell line. However, TCDD exhibits high toxicity toward cells and has unknown activities except for its action as an AhR agonist. In the present study, we tried to clarify how an endogenously generated AhR agonist affects mouse B cell differentiation and functions in terms of the direct effects on the expression of Ig subclasses in purified mouse B cells stimulated with an anti-CD40 antibody and interleukin-4. The AhR agonist 2-(1'H-indole-3'- carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE), which is derived via tryptophan metabolism, suppressed the expression of not only IgM, but also IgG1 and IgE. ITE was also found to suppress the expression of secreted-type Ig mRNAs and plasma cell-specific genes. These findings indicate that the endogenous AhR agonist suppresses B cell differentiation into Ig-secreting plasma cells.抄録全体を表示PDF形式でダウンロード (931K) -
Yunyan Wu, Fuyuki Sato, Ujjal Kumar Bhawal, Takeshi Kawamoto, Katsumi ...2012 年 33 巻 2 号 p. 75-82
発行日: 2012年
公開日: 2012/04/26
ジャーナル フリーDEC1 (BHLHE40/Stra13/Sharp2) and DEC2 (BHLHE41/Sharp1) are basic helix-loop-helix (bHLH) transcription factors that are involved in the regulation of apoptosis, cell proliferation, circadian rhythms and the response to hypoxia. We previously showed the functional effects of DEC1 and DEC2 on apoptosis in human breast cancer MCF-7 cells. However, the roles of DEC1 and DEC2 in oral cancer are poorly understood. We examined whether DEC1 and DEC2 are involved in the regulation of apoptosis in human oral cancer HSC-3 and CA9-22 cells. The expression of DEC2 was upregulated by cis-diamminedichloroplatinum (II) (cisplatin: CDDP) treatment in HSC-3 cells, whereas CDDP treatment had little effects on the expression of DEC2 in CA9-22 cells. We showed that DEC2 overexpression inhibits pro-apoptotic factor Bim and inhibits apoptosis induced by CDDP in HSC-3 cells, whereas it had little effects on apoptosis in CA9-22 cells. DEC1 overexpression had little effects on apoptosis induced by CDDP in these cells. We also found that CDDP upregulated the amounts of DEC2 in the nucleus in HSC-3 cells. These results suggest that DEC2 has anti-apoptotic effects on apoptosis induced by CDDP in HSC-3 cells.抄録全体を表示PDF形式でダウンロード (979K) -
Wei Zhang, Toshishige Shibamoto, Yuhichi Kuda, Shohei Shinomiya, Yasut ...2012 年 33 巻 2 号 p. 83-88
発行日: 2012年
公開日: 2012/04/26
ジャーナル フリーVasopressin, a vasoactive peptide, causes vasoconstriction via V1a vasopressin receptors. Unlike other vasoconstrictor agents, vasopressin also has vasodilatory properties. The purpose of this study was to determine the effect of vasopressin on hepatic and splanchnic circulation in Sprague- Dawley rats. The experiments were conducted in not only isolated blood- and constant flowperfused livers but also anesthetized spontaneously breathing rats. In anesthetized rats, portal venous pressure (Ppv), systemic arterial pressure (Psa), central venous pressure, and hepatic blood flow (HBF) of combined portal venous and hepatic arterial blood flow were continuously measured, and splanchnic vascular bed resistance (Rspl) defined by (Psa - Ppv) / HBF was determined. In perfused livers, vasopressin at 0.1-1,000 nM caused weak venoconstriction as evidenced by small increase in Ppv. In anesthetized rats, when vasopressin was injected into the portal vein as a bolus consecutively at 0.01-100 nmol/kg, Psa increased dose-dependently with the peak increment of 60 ± 18 mmHg at 100 nmol/kg. Ppv and HBF decreased, with resultant increase in Rspl, indicating splanchnic vasoconstriction. In conclusion, hepatic venoconstrictor action of vasopressin was weak in rats. Vasopressin causes splanchnic vasoconstriction, resulting in a decrease in HBF and Ppv in anesthetized rats.抄録全体を表示PDF形式でダウンロード (582K) -
Kana Sasaki, Toshiki Matsunaga, Takenori Tomite, Takayuki Yoshikawa, Y ...2012 年 33 巻 2 号 p. 89-96
発行日: 2012年
公開日: 2012/04/26
ジャーナル フリーHemiplegia is a common sequel of stroke and assisted living care is needed in many cases. The purpose of this study was to evaluate the effect of using surface electrode stimulation device in rehabilitation, in terms of functional improvement in upper limb and the changes in brain activation related to central nervous system reconstruction. Five patients with chronic hemiplegia received electrical stimulation therapy using the orthosis-type surface electrode stimulation device for 12 weeks. Training time was 30 min/day for the first weeks, and increased 30 min/day in every 4 weeks. Upper limb outcome measures included Brunnstrom stage, range of motion, Fugl-Meyer assessment and manual function test. Brain activation was measured using functional MRI. After therapy with therapeutic electrical stimulation (TES) for 12 weeks upper limb function improved in all cases. The results of brain activation showed two patterns. In the first, the stimulation produced an activity in the bilateral somatosensory cortices (SMC), which was seen to continue over time. The second, activation was bilateral and extensive before stimulation, but localized to the SMC after intervention. Treatment with TES using an orthosis-type electrode stimulation device improves upper limb function in chronic hemiplegia patients. The present findings suggest that there are not only efferent but also afferent effects that may promote central nervous system remodeling.抄録全体を表示PDF形式でダウンロード (854K) -
Hirokazu Isobe, Tomomi Takano, Akihito Nishiyama, Wei-Chun Hung, Shuic ...2012 年 33 巻 2 号 p. 97-109
発行日: 2012年
公開日: 2012/04/26
ジャーナル フリーMethicillin-resistant Staphylococcus aureus (MRSA) includes hospital-acquired MRSA (HAMRSA) and community-acquired MRSA (CA-MRSA). Panton-Valentine leukocidin (PVL)-positive multilocus sequence type 30 (ST30) MRSA is one of worldwide CA-MRSA, which has also persisted in Japan since the 1980s. However, unexpectedly, it was not the same ST30 clone throughout. Before 2000, it was HA-MRSA with spa43 and φSa3sea (phage Sa3 carrying the sea gene) and only one PVL-positive MRSA in Japan; in the 1980s, ST30 MRSA accounted for 23.5% of HA-MRSA, showed multidrug resistance, had high MICs for oxacillin and imipenem, and caused decubitus and pneumonia in hospitalized patients. A dynamic clonal change (spa43/φSa3sea→ spa19) occurred around 2000-2002. A rare spa43/φSa3sea/SCCmecI-IE25923 genotype also emerged. After 2002, the prevalent spa19 clone was CA-MRSA; it accounted for only 0.3% (or less) of MRSA in hospitals but 7.6% of CA-MRSA. Since 2007, PVL-positive CA-MRSA with other ST types (such as ST8, ST22, and ST59) also emerged in Japan, albeit at a low frequency. ST30/spa19 CA-MRSA occasionally caused severe invasive infections and a novel ST1335/spa19 genotype emerged. These ST30/spa19 CA-MRSA and variants were identified by pulsed field gel electrophoresis. Further analysis revealed that PVL-positive ST30/spa19 CA-MRSA is a highlyvirulent, successful clone, having a potential of clonal expansion.抄録全体を表示PDF形式でダウンロード (660K) -
Shunsuke Miyazaki, Naoki Kitamura, Aiko Nishio, Saki Tanaka, Tomohiko ...2012 年 33 巻 2 号 p. 111-118
発行日: 2012年
公開日: 2012/04/26
ジャーナル フリーGymnopilus junonius is a widely spread mushroom in Japan and well known as a hallucinogenic mushroom. Gymnopilin was purified from the fruiting body of G. junonius and was reported to act on the spinal cord and depolarize motoneurons. This is the only evidence that gymnopilin has a biological effect on animals and no mechanism of the action has been determined at all. In this study, we examined effects of gymnopilin on intracellular Ca2+ concentrations ([Ca2+]i) of cultured cells isolated from the dorsal root ganglion (DRG) of the rat. The cell culture consisted of neurons and non-neuronal cells. Gymnopilin increased [Ca2+]i in both the types of cells. The gymnopilinevoked [Ca2+]i rise in the non-neuronal cells was inhibited by cyclopiazonic acid and U-73122, inhibitors of Ca2+-ATPase of the intracellular Ca2+ store and phospholipase C, respectively, but not by removal of extracellular Ca2+. These results indicate that gymnopilin activated phospholipase C and mobilize Ca2+ from the intracellular Ca2+ store in non-neuronal cells from the DRG. This is the first report to show that gymnopilin directly acts on cells isolated from the mammalian nervous system.抄録全体を表示PDF形式でダウンロード (2115K) -
Angela Quispe-Salcedo, Hiroko Ida-Yonemochi, Mitsushiro Nakatomi, Haya ...2012 年 33 巻 2 号 p. 119-132
発行日: 2012年
公開日: 2012/04/26
ジャーナル フリーDifferentiated odontoblasts could not be identified by one unique phenotypic marker, but the combination of expression of dentin phosphoprotein (Dpp), dentin sialoprotein (Dsp), dentin matrix protein 1 (Dmp1), and nestin may be valuable for the assessment of these cells. However, the findings using these proteins remain controversial. This study aimed to compare two odontoblast differentiation markers: nestin and Dsp in the process of dentinogenesis in mice. We performed immunohistochemistry and/or in situ hybridization technique for nestin and Dsp using 3-week-old incisors as well as postnatal 1-day- to 8-week-old molars. Preodontoblasts began to express nestin and Dsp proteins and Dsp mRNA, which increased in their intensity according to the progress of odontoblast differentiation in both incisors and developing molars. Nestin was consistently expressed in the differentiated odontoblasts even after the completion of dentin matrix deposition. The expression of Dsp mRNA coincided with the odontoblast secretory activity for dentin matrix deposition. In contrast, other pulpal cells, predentin matrix and dentinal tubules also showed a positive reaction for Dsp protein in addition to differentiated odontoblasts. In conclusion, nestin is valuable as a differentiation marker for odontoblasts, whereas Dsp mRNA is a functional marker for their secretory activity.抄録全体を表示PDF形式でダウンロード (1639K)
Communications
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Isao Nadaoka, Masaaki Yasue, Manabu Sami, Yasushi Kitagawa2012 年 33 巻 2 号 p. 133-137
発行日: 2012年
公開日: 2012/04/26
ジャーナル フリーWe investigated the effects of Cimicifuga racemosa (CR) plant extracts on the changes in levels of the cerebral monoamines norepinephrine (NE), dopamine (DA), and serotonin (5-HT), the respective metabolites 3-methoxy-4-hydroxyphenylglycol (MHPG), 3,4-dihydroxyphenylacetic acid (DOPAC), and 5-hydroxyindoleacetic acid (5-HIAA), and plasma corticosterone in mice subjected to acute immobilization stress. Single oral administration of the CR extract (1,000 mg/kg) significantly attenuated plasma corticosterone levels that had been increased as a result of enforced immobilization. Acute immobilization stress caused significant changes in the corresponding amine-to-metabolite ratios in the hypothalamus, hippocampus, and cortex; however, CR-extract treatment significantly attenuated the MHPG/NE change in the hypothalamus, and the 5-HIAA/5- HT changes in each region of the brain. Our results suggest that the CR extract interacts not only with the hypothalamic-pituitary-adrenal (HPA) axis but also with the sympathetic adrenomedullary (SAM) system under stress conditions. Thus the CR extract can alleviate acute stress responses by suppressing the changes of amine-to-metabolite ratio in brain.抄録全体を表示PDF形式でダウンロード (400K) -
Yoko Motofuji, Asako Saito, Morio Koike, Yoshio Kodera, Tadakazu Maeda ...2012 年 33 巻 2 号 p. 139-143
発行日: 2012年
公開日: 2012/04/26
ジャーナル フリーThis is the first report to describe the potential for classification of cancer using anti-phosphoprotein monoclonal antibodies (PPmAbs) and multiple discriminant analysis. Over 150 hybridoma clones producing monoclonal antibodies were generated against a human phosphoprotein mixture derived from a human leukemia cell line. The expression profiles of 22 cell lines from 9 different types of cancer using PPmAbs were examined. The relationship between cancer cells and the expression of human phosphoprotein in the cells was analyzed by multiple discriminant analysis and was used to construct a diagnostic system for cancers. Multiple discriminant analysis was able to successfully classify the cell lines into the correct cancer group by using the diagnostic system for cancers. These results show that multiple discriminant analysis based on phosphoprotein expression in cells or tissues may be a potentially valuable method for assisting in the classification of several cancers.抄録全体を表示PDF形式でダウンロード (515K)
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