Biomedical Research
Online ISSN : 1880-313X
Print ISSN : 0388-6107
ISSN-L : 0388-6107
9 巻 , 4 号
選択された号の論文の10件中1~10を表示しています
Full Papers
  • S. K. COLES, H. E. HOFF, ROBERT E. SCHUHMANN
    1988 年 9 巻 4 号 p. 247-252
    発行日: 1988/08/01
    公開日: 2016/09/02
    ジャーナル フリー

    Fifteen cats and five dogs were decerebrated at the midcollicular level and then cordotomized at the twelfth thoracic level. We observed an abrupt augmentation of respiration as a previously undefined effect within the Schiff-Sherrington phenomenon in decerebrate cats immediately upon the transection of the spinal cord, averaging a 56% increases in minute volume (P<0.005). The results are interpreted as pointing to the presence of a neuromechanism linking the increased ventilation with enhanced somatic neuromuscular effects and/or by the release of postural inhibitory reflexes in the Schiff-Sherrington phenomenon as accompanied by the release of respiratory activity, or some combination of systems.

  • KATHARINA SPANEL-BOROWSKI, UERIKE SCHNAPPER, BERNO HEYMER
    1988 年 9 巻 4 号 p. 253-260
    発行日: 1988/08/01
    公開日: 2016/09/02
    ジャーナル フリー

    The chick chorioallantoic membrane (CAM) assay was applied for the determination of angiogenic substances. The pseudo-peroxidase activity of erythrocytes was used as a marker of blood capillaries growing in CAM during days 6-14 of incubation. The CAM reaction induced by eye contact lenses, agarose gels, egg shell membranes, and Millipore and glass fiber filters implanted during days 6-10, 6-14, or 10-14 of incubation, was examined by light microscopy. Contact lenses, agarose gels and Millipore filters were soaked in [3H]histamine. The amount of radioactivity was determined in the supernatant of each carrier material incubated in vitro for 4 days, or in the CAM implants during days 10-14 of incubation. Formation of the capillary network in the chorionic epithelium was not complete on day 10 of incubation, but was complete on day 11. Contact lenses always caused capillary involution in the chorionic epithelium. Different carrier materials caused various types of inflammation, i.e. erosive, ulcerous or the granulomatous and ulcerous. In general, the carriers elicited a stronger inflammation when implanted during days 10-14 than during 6-10 or 6-14 of incubation. [3H]Histamine was released continuously from carrier materials during 4 days of incubation in vitro. In contrast, [3H]histamine release from implants in vivo lasted only for 1 day. We conclude that data obtained by the CAM assay are subject to certain restrictions.

  • HITOSHI OKAMURA, KUNIO KITAHAMA, BRIGITTE RAYNAUD, IKUKO NAGATSU, CARL ...
    1988 年 9 巻 4 号 p. 261-267
    発行日: 1988/08/01
    公開日: 2016/09/02
    ジャーナル フリー

    In the rat hypothalamic tuberal region, the distribution of aromatic L-amino acid decarboxylase (AADC)-immunoreactive cells was compared with that of tyrosine hydroxylase (TH)-immunoreactive cells. Two types of AADC-immunoreactive cells were found in the tuberal region; strongly stained cells with distinct processes and weakly stained cells visualized only after colchicine treatment. Most of the intensely AADC-immunoreactive cells were restricted to the dorsomedial arcuate nucleus and neighbouring periventricular nucleus. The second type of AADC-immunoreactive cells was found in the ventromedial and lateral parts of the nucleus. In the ventrolateral part of the arcuate nucleus and its neighbouring periarcuate region, we identified a dense aggregate of TH-immunoreactive cells, but there were no AADC-immunoreactive cells in the homologous region.

  • TAKUO NAKANO, PAUL G. SCOTT
    1988 年 9 巻 4 号 p. 269-279
    発行日: 1988/08/01
    公開日: 2016/09/02
    ジャーナル フリー

    Two enzymes capable of degrading the protein core of proteodermatan sulphate were isolated and partially purified in latent form from medium conditioned by the culture of fibroblasts from human gingiva. One of these enzymes had a pH optimum of close to 7 and the other of close to 6. The molecular weight of the latent ‘neutral’ proteinase, determined by gel-filtration chromatography, was 48,000, decreasing to 37,000 and 19,000 on activation by limited proteolysis with trypsin. For the ‘acidic’ proteinase the molecular weights for latent and active forms were 68,000 and 57,000, respectively. Both enzymes were inhibited by ethylene-diaminetetra-acetic acid and by 1,10-phenanthroline but not by N-ethylmaleimide, phenylmethanesulphonyl fluoride, leupeptin or pepstatin. They are therefore probably metalloproteinases. Limited digestion of proteodermatan sulphate with either enzyme yielded a large fragment including about 200 amino acids from the aminoterminus and the dermatan sulphate chain, together with a number of smaller fragments derived from the C-terminal portion of the core. Prolonged digestion resulted in conversion of the large fragment to small peptides.

  • HITOSHI MIYAZAKI, MOTOHIRO KONDOH, JUNJI OHNISHI, YASUSHI MASUDA, SHIG ...
    1988 年 9 巻 4 号 p. 281-285
    発行日: 1988/08/01
    公開日: 2016/09/02
    ジャーナル フリー

    Scatchard analysis of angiotensin II (A II) binding data revealed different effects of dithiothreitol (DTT) on high-affinity AII receptors in the bovine adrenal and ovary. Dithiothreitol predominantly inhibited 125I-A II binding capacity of adrenal membranes by decreasing the number of the high-affinity binding sites (Bmax=265±5 fmol/mg of protein without DTT; Bmax=26±5 fmol/mg of protein with 100 mM DTT) without significant change in A II binding affinity (Kd=0.49±0.02 nM vs. Kd=0.37±0.10 nM). By contrast, in the ovary the agent stimulated 125I-A II binding capacity up to 3-fold by increasing A II binding affinity of the high-affinity receptors (Kd=0.22±0.04 nM vs. Kd=0.041±0.015 nM for 0 mM and 100 mM DTT, respectively) with no change in the number of binding sites (Bmax=72±13 vs. 85±8 fmol/mg of protein). These results suggest the existence of two distinct subtypes of A II receptors between the two tissues.

  • TORU ISHIKAWA, TOMIO KANNO
    1988 年 9 巻 4 号 p. 287-304
    発行日: 1988/08/01
    公開日: 2016/09/02
    ジャーナル フリー

    To elucidate mechanisms of elevated resting fluid secretion in hypertrophied pancreas produced by the oral administration of a synthetic protease inhibitor (FOY-305) once a day for 3 weeks, the influence of the ionic environment and transport blockers was studied in the isolated perfused rat pancreas. The rate of resting fluid secretion of the perfused hypertrophied pancreas during the perfusion period of 3 h was 7 times higher than that of the control pancreas. The elevated resting fluid secretion in the hypertrophied pancreas was reduced by perfusion with K+-free, low Na+, low Cl- or HCO3--free solution. The elevated fluid secretion was reduced by perfusion with ouabain or amiloride, but was not by furosemide or SITS. The elevated fluid secretion was reduced by anoxic treatment or by perfusion with 2,4-dinitrophenol. The inhibition of the hypertrophied pancreas in various ionic environments and with various inhibitory agents was not observed in the perfused control pancreas. These results led us to conclude that the enhanced level of resting fluid secretion in the hypertrophied rat pancreas may be maintained by three major transport systems working cooperatively each other; i.e. NaK-ATPase activity, amiloridesensitive Na-H antiport system, and a unique Cl- transport system, which is insensitive to furosemide and to SITS.

  • QI MEI
    1988 年 9 巻 4 号 p. 305-317
    発行日: 1988/08/01
    公開日: 2016/09/02
    ジャーナル フリー

    The relation between gastrin-releasing peptide (GRP)-immunoreactive nerves and development of stomach ulcers was studied in rats. The ulcer was induced by exposure of animals to restraint plus water immersion or by administration of insulin, serotonin, aspirin, indomethacin or ethanol. Immunohistochemistry demonstrated a network of nerves exhibiting the immunoreactivity for GRP in the oxyntic mucosa of normal animals. A marked decrease in number of the GRP-immunoreactive nerves was seen to accompany with the ulcers induced by the restraint stress or by insulin. On the other hand, no appreciable change in number of the GRP-immunoreactive nerves was found in ulcers induced by indomethacin, serotonin, ethanol or aspirin. Vagotomy prevented not only the development of ulcers by stress or insulin, but also the decrease in the GRP-immunoreactive nerves. Electrical stimulation of the vagal nerve resulted in ulcer formation and also caused a marked decrease in number of the GRP-immunoreactive nerves in the oxyntic mucosa. The results suggest the possibility that depletion of GRP from nerves in the oxyntic mucosa may be involved in the development of stress or insulin ulcers and that such a release of GRP may be caused by hyperactivity of the vagal nerve.

  • TOMOATSU KIMURA, NATSUO YASUI, SHIGEYUKI WAKITANI, NOBUHITO ARAKI, KEI ...
    1988 年 9 巻 4 号 p. 319-324
    発行日: 1988/08/01
    公開日: 2016/09/02
    ジャーナル フリー

    Changes in the level of type IX and type II collegen mRNA were examined during the course of chick limb development in vivo using cloned cDNA probes. Substantial amounts of type IX and type II collagen mRNA were detected at stage 26, concurrently with the chondrogenic differentiation of limb mesenchyme. Subsequently, the amounts of type IX and type II collagen mRNA showed parallel and marked increases corresponding to the progressive accumulation of cartilage matrix in the limb. Type IX and type II collagen synthesis also increased coinciding with a dramatic increase in their respective mRNAs. The present study suggests that type IX and type II collagens are coordinately expressed during the course of limb cartilage differentiation, which would enable the formation of a very precise temporal and spatial arrangement of cartilage matrices.

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