A simple method for analysing theoretically optical absorption curves and fluorescence curves of largae biomolecules is given. By this, an origin of the vibrational structure in spectral curves can be easily made clear in respective molecules, and assignment of the vibrational mode which contributes to the vibrational structures becomes also possible. As an example, analysis of the optical absorption curves of β-carotene is shown.
From such an analysis, information of the change in the molecular structure accompanying the electronic transition and of the dynamical properties of the molecule in the solution can be obtained. Result of extensive applicaiion to biomolecules would be given in future.

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The present review deals with slow trangent phenomena in the enzyme reaction accompanied by time lag or burst phase in a range of time scale from several minutes to one second in the initial time course: the observation of these phases may not require any special techniques for rapid reaction measurement.
The phenomenon is often observed in oligomeric regulatory enzymes rather than in monomeric enzymes.
The phenomenon may involve a slow conversion of one form of the enzyme to a different form (s) with different activity. The reaction mechanism illustrating such phenomenon is discuseed in connection with cooperativity (sigmoidal relation between steady-state rate and ligand concentration). In addition, monomeric enzyme reactions with slow trangent phases and enzyme reactions with the trangents causing in ligand side but not in the enzyme are also reviewed.

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