巻号一覧
22 巻, 1 号
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高乗 仁1982 年 22 巻 1 号 p. 13-23
発行日: 1982/01/25
公開日: 2009/05/25
ジャーナル フリーThe yeast Saccharomyces cerevisiae contains 50-100 copies of an autonomously replicating DNA molecule named 2μm DNA. The 2μm DNA is a circular molecule of 6, 300 base-pairs long and contains two invert repeats of 600 base-pairs which are separated from each other by unique segments of 2, 300 and 2, 800 base-pairs. The extensive genetic studies revealed that the replication of 2μm DNA quite resembles that of the yeast chromo- some: each of the 2μm DNA molecule replicates once in each cell cycle, and all of the known gene products involved in the chromosomal replication are also required for the 2μm DNA replication. The 2μm DNA therefore provides an excellent model system for studying the mechanism of eucaryotic chromosome replication.
The in vitro system for the 2μm DNA replication recently developed has elucidated the following characteristics. 1) The replication mostly starts from the specific site which is identical with the in vivo replication origin. 2) The mode of replication is bidirectional and discontinuous. 3) The in vitro 2μm DNA synthesis is temperature sensitive in the extracts prepared from a cell division cycle mutant, cdc 8.
These results indicate that the reactions in the in vitro system reflect the in vivo replication and that proteins participating in the replication of 2μm DNA as well as the yeast chromosome can be characterized by using this in vitro system.抄録全体を表示PDF形式でダウンロード (2280K) -
八木 直人1982 年 22 巻 1 号 p. 24-32
発行日: 1982/01/25
公開日: 2009/05/25
ジャーナル フリーThe structure of a light meromyosin (LMM) tactoid with a 43-nm periodicity was studied by X-ray diffraction and electron microscopy.
A strong magnetic field was applied to obtain oriented specimens of tactoids for X-ray diffraction. The tactoid was found to contain a planer rectangular lattice with a unit cell of 6.5×3.9nm in a plane perpendicular to its long axis which is parallel to the length of LLM molecule. Electron microscopy showed that the tactoid was made of sheet-like units which had a planer lattice of 3.6×86nm.
From these observations a model for the molecular packing in LMM tactoid was deduced. In this model the tactoid is made of a stacking of Unit layers. A Unit Cell (6.5×3.9×86nm) contains tour LMM molecules, each of which is 90nm long and packed co-planar. The molecules make parallel interactions with staggers of 86 and 43nm and anti-parallel interactions with overlaps of 84 and 41nm. The two parallel interactions may play an important role in the formation of the thick filament backbone if it has a rope-like struncture.抄録全体を表示PDF形式でダウンロード (3433K) -
1982 年 22 巻 1 号 p. 34-39
発行日: 1982/01/25
公開日: 2009/05/25
ジャーナル フリーPDF形式でダウンロード (403K) -
海野 修, 小池 巧起1982 年 22 巻 1 号 p. 43-45
発行日: 1982/01/25
公開日: 2009/05/25
ジャーナル フリーPDF形式でダウンロード (1388K)
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