Seibutsu Butsuri
Online ISSN : 1347-4219
Print ISSN : 0582-4052
ISSN-L : 0582-4052
Volume 31, Issue 1
Displaying 1-8 of 8 articles from this issue
  • Hiroyuki KOIKE, Yorinao INOUE
    1991 Volume 31 Issue 1 Pages 1-7
    Published: January 25, 1991
    Released on J-STAGE: May 25, 2009
    JOURNAL FREE ACCESS
    Thermoluminescence is a burst of light emission by warming the leaf or chloroplasts which were illuminated by light. It results from the charge recombination between positively and negatively charged pairs created by photochemical reaction. The origin of four out of seven thermoluminescence bands was assigned: the A, Q, B1 and B2 bands arise from S3QA-, S2QA-, S3QB-·and S2QB- charge pairs, respectively. Thermoluminescence is applied to the analysis of oxidinzing and reducing side of photosystem II. The following facts were elucidated; 1) By removal of 33 kDa extrinsic protein, S3 to S4 transition is inhibited. 2) By Cl-·depletion, abnomal S2 state is formed. 3) Replacement of QB-quinone with quinone derivatives yields a modified thermoluminescence band. 4) Thermoluminescence is a powerful tool for the study of herbicides and the resistant mutants.
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  • Teruo SHIMMEN
    1991 Volume 31 Issue 1 Pages 8-12
    Published: January 25, 1991
    Released on J-STAGE: May 25, 2009
    JOURNAL FREE ACCESS
    Techniques were developed to induce sliding movement of foreign myosin along the characean actin cables. Since characean actin cables per se have no Ca2+ sensitivity, it is possible to analyze myosin-linked regulation of actin-myosin sliding by Ca2+. It is also possible to study sliding movement of myosin without purification of myosin. Usefulness of this technique was discussed.
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  • Akira TAKAI
    1991 Volume 31 Issue 1 Pages 13-17
    Published: January 25, 1991
    Released on J-STAGE: May 25, 2009
    JOURNAL FREE ACCESS
    Okadaic acid is the first exogenous substance that has been described to have a potent and specific inhibitory effect on type 2A-, type 1- and type 2B- protein phosphatases, whose catalytic subunits have a striking sequence homology. Of these types of enzyme, type 2A-phosphatase is most strongly inhibited by okadaic acid. The dissociation constant for the interaction between this enzyme and okadaic acid is estimated to be 30pM. As expected from the kinetic theory for tightly binding inhibitors, the dose-inhibition relationship is markedly shifted to the right and became steeper when the concentration of the enzyme is increased. The concentrations of okadaic acfd for 50% inhibition of type 1 and type 2B-phosphatases are 200 nM and 5 μM, respectively. Kinetic studies have shown that okadaic acid acts as a non-competitive or mixed inhibitor on the okadaic acid-sensitive enzymes.
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  • Tadanao ITO
    1991 Volume 31 Issue 1 Pages 18-23
    Published: January 25, 1991
    Released on J-STAGE: May 25, 2009
    JOURNAL FREE ACCESS
    "Osmoelastic coupling", a new thermodynamic phenomenon which was found by us, is reviewed. "Osmoelastic coupling" is a coupling between elasticity of solute and osmotic stress. When a solution containing an elastic solute such as F-actin is subjected to osmotic stress across a semipermeable membrane, the solute is elastically compressed to produce elastic pressure which counteracts the osmotic stress (osmoelastic coupling). Such coupling preferentially increases the free energy of the solute surrounded by solvent, and above a critical intensity of osmotic stress, the solute takes an aggregate form to elude the effect of osmotic stress (osmophobic association). "Osmoelastic coupling" and "osmophobic association" also take place in a solution in which a macromolecular component is heterogeneously distributed.
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  • Akira YASUI
    1991 Volume 31 Issue 1 Pages 24-29
    Published: January 25, 1991
    Released on J-STAGE: May 25, 2009
    JOURNAL FREE ACCESS
    Large amount of information saved in DNA is protected by various DNA repair systems in living cells. Photoreactivating enzyme repairs UV-induced pyrimidine dimers by using the energy of blue light. This enzyme has two different light-absorbing elements, one of which is common in various organisms, a reduced FAD. Extensive homology among amino acid sequences of the enzymes from various organisms indicates the ancient origin of this DNA repair system.
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  • Takash MANABE, Shigeru TERABE
    1991 Volume 31 Issue 1 Pages 30-33
    Published: January 25, 1991
    Released on J-STAGE: May 25, 2009
    JOURNAL FREE ACCESS
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  • Kenji KUBA
    1991 Volume 31 Issue 1 Pages 34-37
    Published: January 25, 1991
    Released on J-STAGE: May 25, 2009
    JOURNAL FREE ACCESS
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  • Seishi KUDO, Yukio MAGARIYAMA, Shin-Ichi AIZAWA
    1991 Volume 31 Issue 1 Pages 38-40
    Published: January 25, 1991
    Released on J-STAGE: May 25, 2009
    JOURNAL FREE ACCESS
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