Already more than 10 years have passed since the first publication of fluorescent Ca
2+ indicator. The method of intracellular Ca
2+ concentration measurement now became one of the standard experimental techniques in the filed of biosciences. The pseudo-color visualization of the Ca
2+ dynamics in the living cell was quite convincing. This can be achieved by the computers and its related techniques which have tremendously developed in those 10 years. In this technical review we will overview the recent been development of fluorescent Ca
2+ indicators and the techniques for image analysis of intracellular Ca
2+ concentration.
View full abstract