巻号一覧
40 巻, 2 号
通巻228号
選択された号の論文の11件中1～11を表示しています
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巻頭言
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田中 豊一2000 年40 巻2 号 p. 79
発行日: 2000年
公開日: 2001/04/26
ジャーナル フリーPDF形式でダウンロード (44K)
解説
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宮脇 敦史2000 年40 巻2 号 p. 83-88
発行日: 2000年
公開日: 2001/04/26
ジャーナル フリーThe green fluorescent protein (GFP) of the jelly fish Aequorea victoria is a spontaneously fluorescent protein that can be incorporated into other proteins by genetic fusion. One of our approaches is to use two GFPs of different colors to permit fluorescence resonance energy transfer (FRET), which is highly sensitive to the relative orientation and distance between the two fluorophores and alters the ratio of their emission intensities. For example, cameleons are genetically-encoded fluorescent indicators for calcium. I describe here potentially unique features of GFP-based FRET, which would allow us to make probes for many intracellular signaling events that are currently assayed by grinding millions of cells.抄録全体を表示PDF形式でダウンロード (360K) -
− 1分子捕捉·操作によるルースカップリングの直接証拠 −喜多村 和郎, 岩根 敦子, 徳永 万喜洋2000 年40 巻2 号 p. 89-93
発行日: 2000年
公開日: 2001/04/26
ジャーナル フリーTo resolve the individual mechanical events of force generation by actomyosin, we have developed a new technique to capture and directly manipulate individual myosin subfragment-1 (S1) molecules using a scanning probe. The unitary mechanical step during sliding along an actin filament is 5.3nm, but 2 to 5 rapid steps in succession often end up with total displacements of ∼11 to ∼30nm. Multiple steps are produced by single heads during the hydrolysis of a single ATP molecule.抄録全体を表示PDF形式でダウンロード (309K)
総説
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金品 昌志, 松木 均2000 年40 巻2 号 p. 94-98
発行日: 2000年
公開日: 2001/04/26
ジャーナル フリーHigh-pressure studies on the bilayer phase transitions of phosphatidylcholines containing linear saturated acyl chains are reviewed. Temperature-pressure phase diagrams of lipid bilayer membranes are shown; the pressure-induced, interdigitated gel phase is found in addition to liquid crystal, ripple gel and lamellar gel phases. The minimum pressure for the interdigitation of lipid bilayers decreases with an increase in acyl chain length in a non-linear manner. Thermodynamic quantities for phase transitions are also discussed.抄録全体を表示PDF形式でダウンロード (250K) -
- 核酸の熱力学的性質に基づくリボザイムの配列設計 -桑原 知子, 藁科 雅岐, 田中 好幸, 多比良 和誠2000 年40 巻2 号 p. 99-104
発行日: 2000年
公開日: 2001/04/26
ジャーナル フリーRibozymes are potential tools for future gene therapy. Recently, we designed an allosterically controllable novel ribozyme (designated maxizyme) that can be transcribed in vivo under the control of a human tRNAVal-promoter. The maxizyme has sensor arms that can recognize target sequences and, in the presence of such a target sequence only, it can form a cavity that can capture catalytically indispensable Mg2+ ions. The maxizyme (but not conventional ribozymes) has extremely high specificity and high-level activity, not only in vitro but also in vivo. To the best of our knowledge, our novel maxizyme is superior to other nucleic acid-based drugs reported to date because of its extremely high substrate-specificity and high cleavage activity.抄録全体を表示PDF形式でダウンロード (315K) -
鵜川 眞也, 島田 昌一2000 年40 巻2 号 p. 105-110
発行日: 2000年
公開日: 2001/04/26
ジャーナル フリーTaste receptor cells located in taste buds respond to gustatory stimuli using a complex arrangement of receptor molecules, signaling cascades, and ion channels. To depolarize taste receptor cells, ionic stimuli, such as salts and acids (protons), interact directly with apically located ion channels, whereas the second messenger systems via specific taste receptors are important in transducing bitter, sweet, and umami stimuli. In this article, we report the isolation of a cDNA encoding a taste receptor for sourness from a rat circumvallate papilla cDNA library by combined homology and functional expression approaches. Taste receptors for other tastes are also mentioned.抄録全体を表示PDF形式でダウンロード (402K) -
真行寺 千佳子2000 年40 巻2 号 p. 111-116
発行日: 2000年
公開日: 2001/04/26
ジャーナル フリーOne of the prominent features of flagellar movement is oscillation (or cyclical beating). The motive force for the bending is the sliding induced by dynein arms between adjacent doublet microtubules in the flagellar axonemes. To generate oscillatory bending, the activity of dynein should be regulated. The central pair apparatus, which locates at the center of the axoneme, seems to be involved in this regulation. Our recent finding of the oscillation of force in dynein revealed that the dynein molecule itself may be related to the oscillation of the flagellar movement.抄録全体を表示PDF形式でダウンロード (367K)
トピックス
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酒井 秀紀2000 年40 巻2 号 p. 117-120
発行日: 2000年
公開日: 2001/04/26
ジャーナル フリーPDF形式でダウンロード (236K)
実験技術
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内山 秀文2000 年40 巻2 号 p. 121-124
発行日: 2000年
公開日: 2001/04/26
ジャーナル フリーPDF形式でダウンロード (189K)
ひとくふう
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佐藤 衛2000 年40 巻2 号 p. 125-127
発行日: 2000年
公開日: 2001/04/26
ジャーナル フリーPDF形式でダウンロード (156K)
談話室
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松橋 通生, 大島 英之2000 年40 巻2 号 p. 128-131
発行日: 2000年
公開日: 2001/04/26
ジャーナル フリーPDF形式でダウンロード (242K)
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