生物物理
Online ISSN : 1347-4219
Print ISSN : 0582-4052
ISSN-L : 0582-4052
41 巻 , 5 号
通巻237号
選択された号の論文の10件中1~10を表示しています
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  • 小森 博文, 和田 千惠子, 三木 邦夫
    原稿種別: 研究論文
    専門分野: 情報学
    2001 年 41 巻 5 号 p. 235-239
    発行日: 2001年
    公開日: 2001/09/29
    ジャーナル フリー
    The RepE initiator protein of F plasmid plays an essential role in initiating DNA replication in mini-F origin. RepE exhibits two major functions; the RepE monomer plays as a replication initiator which binds to direct repeats (iterons) in origin, while the RepE dimer as an autogenous repressor to the repE operator. The crystal structure of RepE monomer (RepE54) as a complex with an iteron DNA was determined as the first 3-D structure for procaryotic initiator proteins. The RepE monomer consists of topologically similar N- and C-terminal domains related by an internal pseudo two-fold symmetry, which has not been expected from its amino acid sequence. Both domains bind to two major grooves of iteron (19bp) with different binding affinities. The C-terminal domain plays the leading role in this binding, while the N-terminal domain has the additional role in dimerization.
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