巻号一覧
46 巻, 3 号
通巻265号
選択された号の論文の10件中1～10を表示しています
- |<
- <
- 1
- >
- >|
巻頭言
-
曽我部 正博2006 年 46 巻 3 号 p. 121
発行日: 2006年
公開日: 2006/05/25
ジャーナル フリーPDF形式でダウンロード (127K)
解説
-
木村 行宏, 小野 高明2006 年 46 巻 3 号 p. 124-129
発行日: 2006年
公開日: 2006/05/25
ジャーナル フリーPhotosynthetic oxygen evolution is one of the most important and fundamental biological processes on the earth, and catalyzed by the Mn cluster composed of 4 Mn and 1 Ca. However, the mechanism of the oxygen evolution is still largely unknown despite of extensive studies. To understand the mechanism, it is essential to know the structure of the Mn cluster and the chemical changes of its ligands including substrate water during the reaction process. Here, we present the recent progress on the study of the oxygen evolution, based on the results mainly from FTIR spectroscopy as well as X-ray crystallography.
抄録全体を表示PDF形式でダウンロード (898K)
総説
-
田口 英樹2006 年 46 巻 3 号 p. 130-136
発行日: 2006年
公開日: 2006/05/25
ジャーナル フリー
電子付録The chaperonin GroEL is an essential molecular chaperone that assists protein folding in the cell. ATP-dependent conformational change of GroEL leads to the stable binding of cochaperonin GroES, forming a cage-shaped complex that accommodates a substrate protein to complete the folding. After the elucidation of the outline of the molecular mechanism over the last decade, now we are ready to answer the important questions; how GroEL encapsulate the substrate protein? How the substrate protein influences the functional cycle of GroEL? What is the role of ATP hydrolysis in the GroEL-assisted folding? Is the folding in the GroEL-ES cavity is same as that in the bulk solution? Here I review the recent progress on the GroEL study and discuss the essential role of chaperonin GroEL.
抄録全体を表示PDF形式でダウンロード (762K) -
相田 拓洋, 伏見 譲2006 年 46 巻 3 号 p. 137-143
発行日: 2006年
公開日: 2006/05/25
ジャーナル フリー
電子付録In vitro protein evolution is comprehended as a hill-climbing process on a fitness landscape in sequence space, just like the protein folding is comprehended as a valley-descending process on an energy landscape in comformation space. In this review, based on a mathematical model for in vitro molecular evolution, we demonstrated an analogy between evolutionary dynamics and thermodynamics and then many concepts in evolutionary dynamics became comprehensible. By introducing thermodynamics-like concepts such as “free fitness”, it is interpreted that evolution is driven in the direction in which free fitness increases. In this process, the evolving population climbs the fitness landscape by absorbing “fitness information” as the negative entropy from the surroundings as an experimental set-up.
抄録全体を表示PDF形式でダウンロード (865K) -
鎌形 清人, 桑島 邦博2006 年 46 巻 3 号 p. 144-149
発行日: 2006年
公開日: 2006/05/25
ジャーナル フリーFolding rates of many globular proteins which exhibit two-state and non-two-state folding have been determined experimentally. Previous studies have focused on the two-state folding, and clarified that the folding rate of two-state proteins depends on the native backbone structure. Here, we performed a statistical analysis of the relationship between the folding rate and structure-based parameters of non-two-state proteins. As a result, we found that the formation rates of both the intermediate and the native state of non-two-state folding similarly depend on the native backbone structure. The comparison of non-two-state and two-state folding suggests that non-two-state folding is more general than two-state folding.
抄録全体を表示PDF形式でダウンロード (716K) -
藤本 健造2006 年 46 巻 3 号 p. 150-153
発行日: 2006年
公開日: 2006/05/25
ジャーナル フリー
電子付録While many methods for enzymatic and chemical ligation of DNA fragment via a native phosphodiester bond or non-native linkages have been demonstrated, there are only a few methods for photoinduced non-enzymatic chemical ligation. The merit of the photochemical ligation avoiding the need for additional reagent is obvious. Furthermore, their actions are controllable within space and time by the choice of proper irradiation methods. The photoligation methods can be used as a tool for DNA engineering and nanotechnology, and as photoregulated diagnostic and therapeutic agents. Therefore, the target in my research program is the development about phototriggered DNA manipulation via intelligent nucleic acids toward for the next generation DNA manipulation.
抄録全体を表示PDF形式でダウンロード (694K)
トピックス
-
野地 博行2006 年 46 巻 3 号 p. 154-158
発行日: 2006年
公開日: 2006/05/25
ジャーナル フリーPDF形式でダウンロード (545K) -
廣明 秀一, 塩澤 久美子, 富井 健太郎2006 年 46 巻 3 号 p. 159-163
発行日: 2006年
公開日: 2006/05/25
ジャーナル フリー
電子付録PDF形式でダウンロード (838K)
理論/実験 技術
-
横田 浩章, 和沢 鉄一, 石井 由晴2006 年 46 巻 3 号 p. 164-168
発行日: 2006年
公開日: 2006/05/25
ジャーナル フリーPDF形式でダウンロード (499K)
用語コラム
-
2006 年 46 巻 3 号 p. 158
発行日: 2006年
公開日: 2006/05/25
ジャーナル フリーPDF形式でダウンロード (40K)
- |<
- <
- 1
- >
- >|