To discriminate between the two subspecies of the Ashy Minivet (
Pericrocotus divaricatus tegimae and
P. d. divaricatus), we used spectrograms to compare acoustic features between subspecific songs. Seven factors of a typical song phrase were assessed for each subspecies (
P. d. tegimae n=17;
P. d. divaricatus n=13). The factors included the following parameters: a) frequency (Hz) of the introductory part, b) frequency of the first peak, c) highest frequency of the phrase, d) frequency of the last peak, e) the duration of the phrase, f) the frequency at the maximum value of power spectrum, and a combination of b) and d). The average value for each parameter was calculated for each subspecies. Five frequency factors of
P. d. tegimae were significantly higher than those of
P. d. divaricatus.
P. d. tegimae tended to sing flatly or in a falling tone, whereas
P. d. divaricatus sang in a rising tone. In order to discriminate the two subspecies, we used two methods. For each spectrogram, we utilized a linear discriminant function, and we compared one of the measurable values and the 95% confidence intervals of that factor. Even when the focal acoustic files had been recorded under some unfavorable conditions, our methods were able to discriminate reasonably well between the two subspecies of Ashy Minivet. Using these methods, all four samples of unknown subspecies which were recorded in Kanagawa Prefecture were identified as
P. d. tegimae.
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