The amylase activity was measured in the several tissues and plasma after pancreatectomy in the rat. Pancreatectomy decreased the a-amylase content of the blood and several tissues in the rat, whereas the amylase activity did not change in the submandibular gland. Especially, the content of the small intestine lost about 99% of its amylase activity as did the small intestinal mucosa. After cholinergic stimulation, the amylase activity in several tissues and plasma tended to increase but the increase was not statistically significant. Only the enzyme activity in the lumen of the small intestine and distal intestine increased substantially after cholinergic stimulation. However, the maximal concentration in the small intestine was still only 2% f that in the normal non-pancreatectomized animal. The data suggest that the absence of the dramatic increase in the amylase activity in the plasma and several tissues that was observed in the normal rat after this pattern of cholinergic stimulation was due to the absence of the potential source of amylase, the pancreas.
Experimental endotoxin shock was induced with 4 mg/kg of purified endotoxin by continuous infusion instead of bolus injection in order to simulate the clinical condition. Abrupt decrease in the mean artery pressure and transient increase in the pulmonary artery pressure, which were usually seen in the initial stage accompanying the bolus injection of endotoxin, did not occur with continuous infusion.
The superior mesenteric fraction rate of cardiac output (CO) in our study showed an increase, which was different from the small intestinal fraction rate of CO shown by Okada et αl. by the micro-sphere method (MS method) accompanying the bolus injection of endotoxin.
These effects might be caused by the difference in the injection method and endotoxin dose. Measurements of the cardiac output, common carotid artery flow, renal artery flow and superior mesenteric artery flow proved that the largest reduction was observed in the renal blood flow.
Forty-seven serial whole-limb angiograms using ioxaglate as the contrast medium were obtained. The contrast column passing through the arteries of the extremities was sequentially analyzed in all of the whole-limb angiograms. Good results could be expected in percutaneous femoral arteriography by the antidromic injection of 30 ml or more of the contrast medium at various speeds ranging from 3 to 4 ml per second.
To analyse the morphological changes of 25 autopsy hearts of 18 males, 7 females including 3 children with congestive cardiomyopathy (COCM), measurements of the heart weight, chamber volume, wall thickness, circumference of the valvular annulus, thickness of the valvular cusp and transverse diameter of the muscle fiber were performed. The mean onset of heart failure was 38 years of age, the mean duration 60 months, the mean age of death 43 years, the mean heart weight 513 g and the mean total chamber volume 286 ml, the mean chamber volume being 89 ml in the left ventricle (LV), 72 ml in the right ventricle (RV), 71 ml in the right atrium (RA) and 55 ml in the left atrium (LA). Various kinds of correlation among these measured parameters were analysed statistically. There was a significant correlation between the heart weight and the total chamber volume (P<0.05), the volume of LV and RV (p<0.05), the volume of RV and RA (P<0.01), the volume of RA and the circumference of the TV annulus (P<0.000 1) and the volume of LA and the circumference of the MV annulus (P<0001). These data were also compared according to the mode of death (arrhythmia versus heart failure) and the duration of survival (more than 5 years versus 5 years or less). No statistically significant differences between the above groups were found except for the total chamber volume.
Hematological assays of inbred specific pathogen-free (SPF) mice of ten different strains inoculated with Friend leukemia virus (FLV) were performed chronologically to assess whether the genetic control of the host may play an important role in viral oncogenicity. Mice strains C57BL/6J, B 10 (H-2b) and B 10D2 (H-2d) were FLV-resistant, BALB/c, DBA/2N (H-2d), RFM (H-2f), AKR and 80% of CBA/JN (H-2k) were FLY-sensitive (polycythemia) and C3H/ He, B10Br and 20% of CBA/JN (H-2k) were FLY-sensitive (anemia). Only the AKR strain mice showed a spontaneous regression of splenomegaly. These results indicate that there is not a strong but a weak correlation between the H-2 haplotype and the reaction to FLV. The main phenomenon in the anemic mice was the monotonous proliferation of the naked blastic cell, whereas that in the polycythemic mice was the enormous increase of the mature erythroblast and the decrease of the naked blastic cell in the later phase. These facts suggest that the naked blastic cell in the mice with polycythemia are reactive and that in the mice with anemia truly neoplastic.