The Shirodkar operation for the treatment of cervical incompetency during pregnancy was modified by Motoi Saito of our department and has been adopted since 1974. This paper describes the operative techniques of the procedure and reviews the results of 34 cases treated by the modification during the last 10 years. The rate of success was 76.5% for the post-operative delivery after completing 37 weeks of gestation, and, if the 17.6% of the viable premature births were included, the fetal salvage rate by the procedure was 94.1 %. The Saito modification procedure provides easy identification of the cervical cerclage ligature and facilitates the complete removal of the suture before or during labor. The 10-year results suggest that the procedure was as effective as the original Shirodkar operation for the prevention of premature birth and fetal loss due to incompetent cervix during pregnancy.
To examine whether the interference of the snout sensory input causes neuromuscular dysfunction in the murine neck muscles, the fine structure of the dorsal neck muscles was studied by electron microscopy. The infraorbital nerves were bilaterally transected in the adult ICR mice (one-month-old), and the animals were sacrificed after postoperative periods ranging from six to 18 months.
Alpha motor end-plates showed remarkable changes characterized by shrunken synaptic boutons containing abundant lysosomes and/or by disappearance of the synaptic boutons. The changes in the muscle spindles were characterized by the sarcolemmal undulation of the intrafusal muscle fibers and the frequent appearance of lysosomes in their sensory terminals. These degenerative changes were observed between nine months and 18 months after neurotomy and intensified with the length of the postoperative period.
From the results of the present study, it was concluded that a reflex pathway exists between the snout receptor organs and the dorsal neck muscles.
4-S-cysteinylphenol (4-S-CP), the S-homologue of tyrosine, has been recently synthesized as a selective chemotherapeutic agent against malignant melanoma and has been shown to be a specific substrate for tyrosinase in vitro In vivo incorporation of 4-S CP into the B16 and Harding Passey (HP) melanomas and the systemic organs have been evaluated by the autoradiographic method .The distribution of the silver grains indicated that 4-S CP was selectively incorporated into both the B16 and HP melanomas. 4-S-CP was excreted mainly from the kidneys and there was an accumulation of 4-S-CP in the reticulo-endothelial system. These results seemed to contribute to the utilization of 4-S-CP and other related compounds as chemotherapeutic agents against malignant melanoma.