The replication origin from pUC119 was inserted into shuttle vector pUFR043, which could be replicated both in Xanthomonas oryzae pv. oryzae and Escherichia coli. The constructed plasmid, pNASC, contained the replication origins from pUC119 and pUFR043, the λ phage packaging site, the partition locus, the locus conferring mobilization ability to the plasmid, the ampicillin resistance gene, the kanamycin resistance gene and the β-galactosidase gene including the multi-cloning site. The copy numbers of pNASC was much higher than that of pUFR043 in E. coli. X. oryzae pv. oryzae was more efficiently transformed with pNASC than with pUFR043. A genomic library of X. oryzae pv. oryzae strain T7174R could be constructed with pNASC. Since the hybrid plasmid from E. coli was much more efficiently recovered by use of this improved vector, this vector should be useful in investigating the pathogenicity and virulence of X. oryzae pv. oryzae.
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