A simple and rapid method using HPLC with postcolumn detection has been developed for the separation and determination of a peroxide mixture.A hydrogen peroxide(H
2O
2), methylhydroperoxide(MHP)and peroxosulfate mixture was chosen for the sample.LC separation was carried out on an Inertsil ODS-2 column(4.6mm i.d.×250mm length)using pH-adjusted distilled water with H
3PO
4 as the mobile phase at a flow rate of 1ml/min.An oxidizing reaction of I
- by peroxide was applied to the postcolumn reaction.Thus, a 10% KI aq.solution at a flow rate of 0.5ml/min was used as the reaction solution.Teflon tubing(0.5mm i.d.×10m length)was used as a reaction coil at 90°C.UV detection was performed at 348nm.The detection limits(S/N=3)for H
2O
2 and MHP were 35ppb and 50ppb, respectively.Under this analytical condition, peroxides and I
- reacted quantitatively.Thus, the determination of various kinds of peroxides is possible by comparing the peak areas with that of a H
2O
2 standard solution, even if the standard substance of the peroxides is not available.Since the proposed system uses only easily available apparatus and reagents, this method is suitable for process control.
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