1) Ascitic NA content of Yoshida sarcoma was measured by means of quantitative investigation of ascitic NA concentration and NA content per cell. From the result obtained in the experiments it was found that both DNA and RNA contents per cell remained within a definite range during the usual proliferation of Yoshida sarcoma, but with aging of the sarcoma there was a diminution in these contents, as opposed to ascitic NA concentration which was found to increase as time wore on, though varying greatly according to individuals.
2) At twenty-four hours following single whole-body exposure to 500r of X-radiation ascitic DNA and RNA concentrations of Yoshida sarcoma were found to be decreased considerably, but the DNA content per cell was found to remain unchanged and RNA content per cell to be increased slightly so far as the surviving cells in the ascites were concerned.

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1) The amounts of metals contained in the first day's pupae after pupation in two melanotic tumorous strains, v tu and st tu, and a non-melanotic tumorous strain, tu-h, in Drosophila melanogaster were determined to compare with those contained in non-tumorous strains, v, st, and Oregon, respectively.
2) The greater amounts of iron and copper were detected in the melanotic tumorous strains, v tu and st tu, as compared with the non-tumorous strains, v, and st, respectively.
3) The amounts of iron and copper contained in the non-melanotic tumorous strain, tu-h was also shown to be greater than those in the Oregon strain.
4) The relation of the formation of the hereditary tumors in D. melanogaster to the transition metals and tryptophan metabolism was discussed.

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1. An electron microscopic observation on the ultrastructures of spontaneous C₃H and DBA strain mice mammary cancer were carried out by the use of ultrathin sectioning method.
2. No morphologically essential differences of the viral particles and the cell structures between the both strains of mammary cancer could be recognized.
3. Intracellular and extracellular forms of virus could be observed within both strains of cancer cell cytoplasm, ductal lumens and intercellular spaces.
In thin sections, the intracellular form of virus is highly dense and shows mostly doughnut-like structure of 60mμ size, while the extracellular form is ovoid or spherical in shape, 100mμ in size and showing a nucleoid of 50mμ size in its center.
4. The extracellular form of the virus seems to correspond with Bittner's milk agent.
5. Other cellular structures which are similar to the changes as seen in virus infected cells such as inclusion bodies, "Matrix like regions" and "Central Fine Granular Regions" were also observed and discussed.
6. Further detailed electron-microscopic observations on spontaneous and experimentally induced mice mammary cancer should be important in the service for ultramorphological comprehension of "Human Cancer".

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1. Under the conditions of the above described experiments, acid violet, auramine, sodium salicylate, 8-oxyquinoline sulfate, auramine G and salicylic acid did not show carcinogenic activity.
2. No marked change in internal organs was found in the rats, hamsters and mice under the conditions of the respective experiments. It may be noted, however, that auramine produced some degeneration of liver cells in mice and salicylic acid a mild atrophy of spleen in rats.

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Selected representatives of quinone and quinoline derivatives were tested as to their effect on the anaerobic glycolysis of Ehrlich carcinoma cell suspension, and a close parallelism was noted between the glycolysis-inhibiting effect and the anti-cancer action, observed in previous experiments, of the respective derivatives. The significance of, and implications from this parallelism were discussed.

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[in Japanese]

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[in Japanese]

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1. O-fraction obtained from tumor tissue inhibited the growth of E. coli but not of Proteus vulgaris.
2. TO-fraction obtained from tumor tissue did not inhibited either the growth or the synthesis of catalase of E. coli.
3. Various divalent or trivalent cations and RNA or DNA removed the growth inhibiting action of O-fraction.
4. Oxygen consumption of E. coli was inhibited by O-fraction regardless of the kind of added substrate.
5. The inhibition of oxygen consumption was removed by magnesium ion and RNA or DNA.
6. The nature of anti-bacterial principle and its mode of action were discussed.

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Starting from TO-fraction, a nucleic acid-free polypeptide fraction obtained by methanol-acetic acid extraction of a crude toxohormone preparation, the active substance was concentrated by means of adsorption on powdered cellulose from 0.1N acetic acid and elution with 0.1N hydrochloric acid. The hydrochloric acid eluted fraction (Peak II) obtained from TO-fraction of rhodamine sarcoma was active in 1mg doses, indicating 10-fold increase in potency. The same fraction obtained from methanol-acetic acid extract of Murphy lymphosarcoma, which was digested with pepsin prior to the adsorption on cellulose, was active in 0.5mg doses.
Crystalline picrate of an active peptide was prepared from TO-fraction of rhodamine sarcoma by the procedure of pepsin digestion and subsequent precipitation with picric acid in a weakly alkaline medium. This crystalline picrate was revealed to be active in 0.1-0.2mg doses for mice.
The amino acid composition of each fraction was determined by paper-DNP technique.
The homogeneity of the crystalline picrate was discussed and the possibility of obtaining more active peptide than that presented in this paper was suggested.

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Some aspects of metabolic pattern of silicotic granuloma produced on the omentum of rats and guinea pigs were studied. Anaerobic glycolysis activity of the slices of rat's silicotic nodule was lower than that of rhodamine sarcoma but higher than those of muscle and liver. In guinea pig's silicotic granuloma, it was weaker than in Croker sarcoma, but stronger than in muscle and liver. Succinoxidase activity of the homogenate of rat's silicotic nodule was weaker than those of liver and spleen, and in guinea pigs, it was weaker than those of liver and muscle but stronger than that of Croker sarcoma.
Any silicic acid of quartz powder, amorphous colloidal silicic acid, and quartz solution had no inhibitory effect on the succinoxidase activity of the homogenate and of the isolated mitochondria of rat's liver.

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