[in Japanese]

View full abstract

Toxohormone prepared from human gastric cancer induced a paralled depression of ferritin iron and liver catalase of mice, and showed no effect on the crystalline beef liver catalase in vitro.

View full abstract

1. DAB had no effect on the crystalline beef liver catalase in vitro.
2. Hemosiderin, ferritin, and ascorbic acid contents in the liver of rats fed DAB for about 30 days were increased as compared with the normal, while catalase, copper, and riboflavin contents were decreased.
3. With the exception of ascorbic acid, the degree of changes of these components in the liver of rats fed DAB was in parallel with the depression of liver catalase.

View full abstract

[in Japanese]

View full abstract

Plasmopheresis accelerated the synthetic activity of DNA in liver cells of the rat, but not in kidney cells. The hypothesis that the serum protein deficiency causes the induction of DNA synthesis in the liver, was supported by such a result. The possible control of liver cell population was also discussed from this point of view.

View full abstract

Potassium salt of 6-carboxy-4-nitroquinoline 1-oxide was tested as to its toxicity, cancericidal action in vitro, and effect in vivo on Ehrlich ascites carcinoma. Compared to the water-insoluble derivatives previously studied, this watersoluble 6-carboxy compound was found to be about 1/10 as toxic and as effective in cancericidal action in vitro. In in vivo tests, the substance in appropriate doses completely destroyed Ehrlich ascites carcinoma when the treatment was started 24 hours after the tumor inoculation. The optimum doses were found to be about twenty times that of 4-nitroquinoline 1-oxide.
It was also found that 6-carboxy-4-nitroquinoline 1-oxide inhibits the glycolytic action of Ehrlich carcinoma cells in vitro at about twenty times the concentration which is necessary for 4-nitroquinoline 1-oxide. However, no corresponding difference was recognized between the two substances as to their power of inhibiting the activity of crystalline aldolase.

View full abstract

Effect on the in vitro incorporation of P³² into nucleic acids of Ehrlich carcinoma cells was tested with two actively anti-cancerous and two inactive quinoline derivatives.
It was found that 4-nitroquinoline 1-oxide and 6-carboxy-4-nitroquinoline 1-oxide, both active, and 4-nitroquinoline, which is inactive, were markedly inhibitory to the incorporation, while quinoline 1-oxide (inactive) showed no such inhibition. A tentative conclusion was reached that the inhibition of P³² incorporation into nucleic acid by this class of compounds may depend upon the presence of nitro radical at the position 4, and may not be related to their anti-cancer action.

View full abstract

The red-coloured component contained in the commercial brands of trypan blue is removed by treatment with hot ethyl alcohol.
By this manipulation, a dye containing relatively small quantities of the red dye component was prepared from the Merck brand to clarify whether the formation of protein-bound DAB in the livers is decreased or delayed in the rats receiving biweekly trypan blue and DAB, when compared with those on DAB alone.
Throughout a dye-feeding period of 20 weeks the bound DAB showed no significant difference in the amount and period of maximum level between trypan bluestained rats and controls.
The red, purple and blue components contained in a solution of trypan blue (Merck) were separated by adsorpto-chromatography on aluminium oxide, and the inhibiting effects of each component on the DAB-carcinogenesis were determined. Of these dye components, the red and purple dyes did not delay the formation of hepatic neoplasia at all, in doses contained in a 1per cent solution of the Merck preparation. The blue dye most closely resembled in its protective effect the branded dye, but the effect by this component was not a clear-cut inhibition, as has been shown to occur previously with the Merck brand.
It could not be stated that the above-mentioned blue dye contains no red and purple components. The blue component was repeatedly purified by a similar adsorpto-chromatography, in order to remove red and purple bye-products. Although the experiment was discontinued when the rats on DAB alone showed a tumour incidence of 71.4per cent, no anticarcinogenic effect was demonstrated in those on this dye preparation and DAB.
In what kinds and in what amounts these bye-products of the commercial brand should be present in trypan blue solution in order to produce minimum toxicity and simultaneously exhibit powerful anticarcinogenic effects, are matters that still remain unclear.

View full abstract

The acid hematein test was applied to the stomach, duodenum, pancreas, kidney, liver and rat fibroblasts in culture, and to the MTK-sarcoma III, the Yoshida sarcoma and the spindle-shaped cell sarcoma. It was found that most normal cells here investigated contained varying amounts of the acid hematein-positive materials in their cytoplasm, while such materials have failed to be demonstrated in solid tumors so far examined. This seems to indicate a significant, physicochemical difference between tumor cells and normal cells with regard to phospholipids within cytoplasmic particles.
Two types of tumor cells, which showed a differential reaction to the acid hematein test, were distinguishable in the MTK-sarcoma III, the Yoshida sarcoma, and the spindle-shaped cell sarcoma in culture.

View full abstract

Morphological and cytochemical experiments have been undertaken in the present study with a hope to learn the nature of phospholipid-containing tumor cells (BH-p type cells). The results obtained indicate that: 1) phospholipids contained in BH-p type cells are of labile nature; 2) resident tumor cells following low-temperature exposure are characterized by phospholipid-rich cytoplasm; 3) with the increase in number of the BH-p type cells in the tumor ascites the tumor growth becomes speedy; and 4) there is a possibility that the BH-p type cells of resident type may correspond to tumor stem-cells which function as the principal progenitors of the tumor.

View full abstract

1) It was concluded that physiologic Mp is produced by the liver, from the fact that Mp content of posthepatic blood was higher than that of prehepatic blood, and serum Mp content decreased in the presence of both clinical and experimental liver damages.
2) Physiologic Mp level was assumed to be metabolized by the phagocytic function of RES, owing to the fact that Mp level increased with lowered phagocytic activity of the RES, and decreased with acceleration of its activity.
3) On the other hand, it was postulated that the increased serum Mp found in malignant tumor was liberated from the tumor tissue itself, by comparing the Mp content of afferent and efferent blood.
4) Further discussion was made concerning the existence of another source (perhaps in the liver) of Mp induced indirectly by the presence of the malignant tumor in addition to the Mp as produced by the tumor tissue, and that the Mp produced by the tumor tissue itself may be connected with the carrier of cancer toxin.

View full abstract