All of the four toxohormone preparations tested in this experiment, which vary in the extracting procedures and in activities, were revealed to have the action of decreasing plasma iron level of rat. The order of magnitude of this activity of four preparations was just the same as that established for their liver catalase depressing activity. But the ratios of the effective doses for the two actions varied from sample to sample.
Concerning the mode of action of toxohormone in decreasing plasma iron it was demonstrated by using ⁵⁹Fe-labeled plasma that the lowering of iron mobilization from its tissue reserve may be the most probable mechanism.

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1. The basic proteins were isolated from human and experimental tumor tissues and their toxohormone activity was tested.
2. The liver catalase activity of mice was lowered within 24 hours following a single intraperitoneal injection of basic proteins.
3. The plasma iron level of rat was reduced within 12 hours following a intraperitoneal injection of basic proteins.
4. The basic protein obtained from normal tissues showed much less toxohormone activities than that of tumor tissues.
5. The catalase inhibiting activity of the basic proteins isolated from tumor tissues was not found in in vitro experiment.

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1. Liver catalase activity of ddN female mice increased in proportion to age to maximum at around 30 days after birth and then remained constant.
2. Liver β-glucuronidase activity of ddN female mice declined from high level of infant mice to minimum at around 20 days after birth and thereafter maintained the constant value.
3. Catalase and β-glucuronidase activities of liver and plasma iron level in CFW and CF1 strain of adult mice were constant and there was no difference in different sex.

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1) Activity of acylase in the liver of rats fed 4-dimethylaminoazobenzene (DAB) was measured by using as substrates acetanilide (AA), diacetyl-L-tyrosine (DAT) and acetylmethionine (AM).
2) Activity of AA acylase in the slightly cirrhotic liver was higher than that of normal liver and even the severe case showed neary the same height as the normal value, whereas the activity in hepatoma was scarcely detected.
3) When DAT was used for acylase test, pathologically changed livers, including hepatoma, showed higher activity than normal liver.
4) AM acylase showed slightly higher activity in the pathological but noncancerous livers than normal value. Hepatoma showed nearly the half value of that.
5) The liver of DAB rats in their fourth week of experiment showed higher activity than normal, tested with any of AA, DAT and AM.
6) Regenerating liver possessed somewhat lesser activity in the test of AM and half value in that of AA.

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1) Activity patterns of acylase in the homogenate of the liver of rats fed 4-dimethylaminoazobenzene were demonstrated with the aid of paper electrophoresis using acetanilide and D, L-acetylmethionine as substrates.
2) The patterns showed a different feature according to the used substrate, i.e., 2 peaks were recognized in the pattern, when acetanilide was tested and 3 were found in the case of acetylmethionine test.
3) No essential difference was found between the patterns of the normal and cirrhotic liver.
4) In the patterns of hepatoma the characteristic peaks were still maintained to some extent, but they disappeared sometimes by flattening in the case of using acetanilide.
5) The patterns obtained from the liver of the carcinogen fed rats in their fourth week of experiment showed the ambiguous separation of the peaks.

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1) Activity patterns of acetylcholinesterase in the liver of rats fed 4-dimethylaminoazobenzene were demonstrated with the aid of paper-electrophoresis.
2) Three peakes were observed in the patterns and there was found no essential difference among the patterns of the normal liver, cirrhotic liver and hepatoma.
3) The activity pattern of the liver of rats on their fourth week of the carcinogen feeding showed uncertain separation of peaks.
4) The activity pattern in sera of rats showed one sharp peak between α₂- and β-globulin fractions and no differences was found between the sera of normal and hepatoma-bearing rats.

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25mg of 3'-Me-DAB dissolved in one ml of corn oil was administered intermittently (usually once a week) to rats for a long period with a help of a stomach tube. Two days after the last administration of the dye, the liver was removed and the protein bound dye, N-demethylase, catalase and xanthine oxidase activities of the liver were measured. The results are summarized as follows:
1) The amount of polar dye reaches a maximum after a few administrations of the dye, then gradually decreases and finally levels off.
2) The catalase activity decreases at first, reaching a minimum, and then gradually recovers to the normal level. The decrease of the catalase in the early period does not seem to be the result of the inhibition by the dye or its metabolite, but due to the lose of the enzyme itself.
3) The N-demethylase and xanthine oxidase seem to behave similarly to the catalase during the course of the dye administration.
4) Then the interpretation for the enzymic behavior observed during the repeated dye administrations was suggested. After the acute toxicological damage period at the beginning, the hepatic cells survived may regenerate, and the regenerated young cells become more and more resistant to the damaging effect by the carcinogen probably through the altered metabolic activity against the carcinogenic aminoazo dye. Finally the liver becomes completely resistant and no more hepatic damage is produced even by the repeated administrations of the dye, so far as the biochemical analysis indicates.
Under the condition described herewith, the hepatic damage does not go far enough and the chance for the formation of the neoplastic cells seems not to be great.

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Using rats, the authors studied the effects of nicotinamide upon the incidence and growth of experimental tumours induced by p-dimethylaminoazobenzene (DAB) and 20-methylcholanthrene (MC). The effect of diphosphopyridine nucleotide (DPN) on the incidence of MC sarcoma was also examined additionally. The incidence and growth of MC sarcoma was delayed by administration of nicotinamide and DPN. In the case of DAB liver cancer, however, the inhibitory effect was very slight. The difference in the effect of nicotinamide on the tumourigenesis of two different carcinogens was discussed.

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1) The inhibitory action of RC4 stock solution on anaerobic glycolysis of Ehrlich ascites tumor cells or their homogenate is antagonized by addition of nicotinamide or DPN. The inhibitory activity of the fresh solution is exceedingly weak.
2) After contacting the ascites tumor cells with RC4 fresh and stock solutions in Hanks' solution fortified with ascites fluid, the tumor cells were transplated to mice, and the anticancer activites of the fresh and stock solutions were compared by observring whether the tumor cells had grown or not in the mice. The fresh solution, even at concentration as will not at all inhibit glycolysis, completely inhibited the growth of the tumor cells, while the stock solution, at a concentration as will perfectly inhibit glycolysis, did not completely inhibit growth of the tumor cells.
3) Forty-eight hours after inoculation of the ascites tumor cells into mice, the fresh or stock solution of RC4 was injected into the peritoneal cavity of each mouse, and their anticancer activity were examined. Only the fresh solution displayed a conspicuous effect.
4) From the above mentioned findings, it has been conculuded that the anticancer activity of RC4 is not related to its glycolysis inhibiting activity. Furthermore, a few more facts were found to suggest that the anti-glycolytic characteristics of ethylenemines have some common features. Hence, the conclusion concerning RC4 may possibly be generalized in the cases of other ethyleneimines.
5) Neither stimulation of alkaline phosphatase nor inhibition of specific ChE is related to the anticancer effect of RC4.

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The correlation between the variation in the glycolysis inhibiting activity and the structural changes when the aqueous solution of RC4 was preserved at 2°C has been examined from the viewpoint of the spectral changes in ultraviolet and infrared absorptions. Participation of a higher order reaction at the rate determining step of the activity variation has been presumed. It is inferred that the ethyleneimino ring is opened along with the progress of the preservation and hydroxyl radical and ether bond newly appear.

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