GANN Japanese Journal of Cancer Research
Print ISSN : 0016-450X
Volume 59, Issue 6
Displaying 1-16 of 16 articles from this issue
  • From Percival Pott to Katsusaburo Yamagiwa
    Folke HENSCHEN
    1968 Volume 59 Issue 6 Pages 447-451
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
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  • Yoshinobu ISHIKAWA, Matsuro FUKUSHIMA, Tsutomu SATO, Minoru KOMABA, Hi ...
    1968 Volume 59 Issue 6 Pages 453-460
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The lymph node cells, spleen cells, thymus cells, and bone marrow cells were prepared from Yoshida sarcoma-resistant rats produced by Mitomycin-C administration and strengthened by challenge of the same tumor. A certain number of these immune cells was transplanted into isologous and allogeneic rats by intravenous or intraperitoneal injection. Then, 105 Yoshida sarcoma cells were injected intraperitoneally on the 2nd, 8th, 20th, and 60th day. The survival rate of the recipients was the highest in the group which received inoculations on the 8th day, and even in the group receiving inoculations on the 60th day, host resistance was recognized by transfer of immune cells against Yoshida sarcoma. There was no difference in the tumor-resistance between the isologous and allogeneic immune cell transfer. When the number of immune cells was increased, survival rate of the recipients also increased. The relationship between transfer of immune cells and R-factor, one of the histocompatibility genes of rats, was discussed.
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  • I. COMBINED IMMUNOCHEMICAL AND ENZYMIC DETERMINATION OF CATALASE IN LIVER CELL FRACTIONS
    Tokuhiko HIGASHI, Kiyoko KASHIWAGI, Kotaro WARABIOKA
    1968 Volume 59 Issue 6 Pages 461-466
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The liver of a rat bearing ascites hepatoma cells (AH-49WH) was fractionated by a method modified after de Duve, and the catalase distribution was studied by both the enzymic and immunochemical determinations.
    The values obtained by the immunochmical assay were in good agreement with those from the enzymic assay except in microsomes and supernatants, where the immunochemical result was higher than the enzymic one.
    The data lead to the conclusion that the decrease of hepatic catalase activity in tumor-bearing rats mainly represents the decrease in the absolute concentration of enzyme protein probably caused by its impaired synthesis.
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  • II. CHROMATOGRAPHIC ANALYSIS OF LIVER CATALASE
    Tokuhiko HIGASHI, Kiyoko KASHIWAGI, Kotaro WARABIOKA
    1968 Volume 59 Issue 6 Pages 467-472
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The liver catalase of rats bearing ascites hepatoma (AH-49WH) was investigated by chromatography on DEAE-cellulose column. Four catalase fractions (I, II, III, and IV) were isolated by different concentrations of sodium chloride in eluting buffer solution, as is the case in the liver catalase of normal rats. Combined cell fractionation and chromatographic study have revealed that the Catalase-III in peroxisomes decreases most significantly in tumor-bearing rats while the Catalase-I and -II remain unchanged under the same conditions.
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  • Iwao HIRONO, Hideki KACHI, Chiken SHIBUYA, Yoshimori NISHIO
    1968 Volume 59 Issue 6 Pages 473-480_1
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Antitumor effect of Actinomycin-D was studied using six strains of transplantable Wilms tumor and ascites hepatoma AH-130 in rats. Four strains which retained the histological feature of Wilms tumor were most susceptible to Actinomycin-D and the growth rate was inhibited, resulting in a definite prolongation of the life span of host animals. However, no prolongation of life span was observed in AH-130 and two strains of Wilms tumor which were already sarcomatous in the histological pattern, though a slight inhibition in their growth rate was observed.
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  • Mahmoud M. EL-MERZABANI, Yoshio SAKURAI
    1968 Volume 59 Issue 6 Pages 481-488
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Distribution of N-methyl[14C]-bis(3-mesyloxypropyl)amine hydrochloride in the tissue was studied with normal and tumor-bearing rats. The tumors investigated were original and a resistant subline of Yoshida sarcoma, and a rat ascites hepatoma AH-7974, Higher radioactivity content was found in general in all the tissues of tumor-bearing rats than those of normal rats under the same dose of administration. The compound showed somewhat specific affinity to the kidney, intestine, spleen, liver, and tumors. Incorporation of radioactivity into the ascites tumor cells of a resistant subline of Yoshida sarcoma and AH-7974 at 1 hour after injection of the compound was, however, about one-half and one-third of that into Yoshida sarcoma cells but there was no marked difference in the case of solid tumor among the three kinds of tumor examined.
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  • Yasuyuki AKAMATSU, Ryoichi IKEGAMI, Koji WATANABE, Masanori KIKUI
    1968 Volume 59 Issue 6 Pages 489-496_3
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    A low-spontaneous leukemic strain C57BL is said to be susceptible to leukemogenic treatment by X-ray irradiation but not to chemical carcinogens. In the present experiment, 136 senile C57BL mice were treated with a single intragastric instillation of 3-methylcholanthrene-olive oil solution and, as a result, leukemia appeared at 62 to 73 weeks of age in 8 of 57 males with 0.25mg, 9 of 39 females with 0.25mg, and 25 of 40 females with 0.5mg methylcholanthrene, or at the rate of 14, 23, and 600%, respectively.
    The majority of the leukemias, i.e., 24 out of 42, was myelogenous in origin but 15 lymphogenous leukemia and three reticulum cell sarcoma were also observed. Five to six animals in each group developed systemic amyloidosis in the spleen, liver, and occasionally in the kidney. This amyloid showed autofluorescence similar to that of methylcholanthrene when a frozen section stained with Hematoxylin was observed under a fluorescent microscope.
    The susceptibility to chemical carcinogen and senility in relation to physiological hematology of this strain of mice was discussed as a possible explanation of leukemogenesis in senile mice. Amyloidosis induced by chemical carcinogen was also examined to investigate its pathogenesis.
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  • Fumio HIRAO, Tomoo FUJISAWA, Eiro TSUBURA, Yasuyuki AKAMATSU, Yuichi Y ...
    1968 Volume 59 Issue 6 Pages 497-505_7
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    For the purpose of inducing experimental lung cancer, either suspension of 3-methylcholanthrene in distilled water or a mixture of 3-methylcholanthrene and 4-nitroquinoline 1-oxide in rabbit plasma was infused into the lower bronchus of the rabbits. The latter was given in combination with subcutaneous injections of 4-nitroquinoline 1-oxide.
    1) Five rabbits developed typical lung cancer accompanying metastases and/or invasion into tissues adjacent to the lung. Metastases and invasion were observed in two with squamous-cell carcinoma and one with undifferentiated cell carcinoma. In the fourth one, the lesions were composed of the feature of squamous-cell carcinoma in some areas and of adenocarcinoma in other areas, and were seen to infiltrate into the diaphragm. The remaining one showed characteristics of adenocarcinoma with invasion into the diaphragmatic pleura. All of these rabbits received 16 to 41 applications of carcinogens over an experimental period of 127 to 397 days.
    2) Twelve out of 32 rabbits having received more than 4 infusions and survived 30 days or more developed adenocarcinoma not accompanied with metastasis and invasion into the adjacent tissues.
    3) Discussion were made with regard to the sites of the application and vehicles used for the carcinogens.
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  • ESPECIALLY ON ITS CORRELATION TO THEIR CHEMICAL STRUCTURE
    Sumio SAKAI, Shoichi TAKADA, Teruo KAMASUKA, Yoshio MOMOKI, Jun-ichi S ...
    1968 Volume 59 Issue 6 Pages 507-512
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Chemical properties and host-mediated antitumor activity of the glucans of various origins were examined to elucidate the relationship between chemical structure and antitumor activity. It was concluded that the glucans, which were prepared from both culture filtrate and mycelia of Sclerotinia sclerotiorum Masse 2404 and which possessed a certain specific structure composed mainly of chains of β-(1→3)-linked D-glucose residues, were most effective in antitumor action. These glucans were markedly effective same as the polysaccharides prepared from the plants of Gramineae reported previously, even in smaller doses such as 5-25mg/kg. The other glucans composed mainly of a polymer of β-(1→3)-linked D-glucose residues and the glucans considered to be nigeran type were moderately effective. The remaining glucans composed of α-configuration showed no effect.
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  • COMPARISON OF EFFECTS AMONG 4-NITROQUINOLINE 1-OXIDE, ACTINOMYCIN-B, AND QUINOXALINE ANTIBIOTICS
    Yoshio HARADA, Norio SUNAGAWA, Ken KATAGIRI
    1968 Volume 59 Issue 6 Pages 513-522_8
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Using light and electron microscopic procedures, the actions of 4-nitroquinoline 1-oxide, Actinomycin-B, and quinoxaline antibiotics (Quinomycin-A and Triostin-C) were examined on cultured JTC-13 cells after 4 hours of incubation with three dose levels ranging from 0.1 fo 10μg/ml of each chemical.
    In the presence of 4-nitroquinoline 1-oxide, cytoplasmic alterations, such as vesicles in mitochondria, multivesicular bodies, and lvsosomes. appeared with a dose of 0.1μg/ml and activity of succinate dehydrogenase in cytoplasm was markedly decreased.
    Intranuclear inclusion bodies and rearraneement of nucleolar components were demonstrated at concentrations more than 1μg/ml of the drug. Accompanied with these changes, there occurred loss of ribonucleoprotein granules in cytoplasm.
    In contact with Actinomycin-B, dispersed nucleoli and chromatin aggregation near nuclear membranes were found by Acridine Orange staining, with a dose of 0.1μg/ml. Ultrastructural changes consisted of coalescence of nucleolonema, aggregates of granular component, and decrease of amorphous materials in nucleoli, with little variances of cytoplasm. Condensed granules were separated completely from the necleolonema within a nucleus on the exposure to 10μg/ml of the antibiotic. In comparison of the action between 4-nitroquinoline 1-oxide and Actinomycin-B, it was found that the former acted indirectly on the nucleic acid synthesis through disturbance of cytoplasmic metabolism, and the actinomycins directly.
    When quinoxaline antibiotics were administered to the cultured cells, the same nucleolar changes without alterations of cytoplasmic organellae, as shown by Actinomycin-B, were observed.
    The activity of Quinomycin-A was similar to that of Actinomycin-B, but that of Triostin-C was one-tenth of that of Actinomycin-B. Thus, this is the first evidence suggesting the similarity of ultrastructural effects between actinomycin and quinoxaline antibiotics at the cultured animal cell level.
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  • Takashi KAWACHI, Yukiko HIRATA, Takashi SUGIMURA
    1968 Volume 59 Issue 6 Pages 523-525
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The inclusion of L-tryptophan in the diet at the level of 1% enhanced markedly the incidence of hepatic tumor in rats administered with N-nitrosodiethylamine.
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  • Ira KLINE, Denis D. TYRER, John M. VENDITTI, Vaman S. WARAVDEKAR, Miri ...
    1968 Volume 59 Issue 6 Pages 527-535
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Mice inoculated subcutaneously with leukemia L-1210 cells succumb with systemic disease before meningeal leukemia is apparent.
    Treatment with Methotrexate suppresses the systemic disease, but leukemic cells cross the "blood-brain barrier" and meningeal leukemia develops. Methotrexate is relatively ineffective against leukemic cells proliferating at this site since it cannot cross the "blood-brain barrier".
    1-β-D-Arabinofuranosylcytosine hydrochloride (cytosine arabinoside) does cross the "blood-brain barrier" and does somewhat control the meningeal disease. Data from survival and bioassay studies showed that cytosine arabinoside was effective against meningeal leukemia L-1210 developed in mice treated with Methotrexate.
    These studies suggest the usefulness of cytosine arabinoside in situations in which the initial chemical agent, for example, Methotrexate fails to maintain remission in a leukemic patient.
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  • I. SPONTANEOUS ELIMINATION OF VIRUS FROM SARCOMA ORIGINALLY ASSOCIATED WITH FRIEND VIRUS
    Reiko TOKUZEN, Waro NAKAHARA
    1968 Volume 59 Issue 6 Pages 537-539
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    A transplantable mouse sarcoma first found in a mouse inoculated with the Friend leukemia virus, which in its early transplantation generations contained the virus, became free of the virus with the progress of serial transplantations. It is suggested that this sarcoma is different from the sarcoma produced by transplantation of the leukemic tissues of the Friend disease.
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  • II. POSSIBLE EFFECT OF NTF SARCOMA ON ACTIVITY AND MULTIPLICATION OF FRIEND VIRUS
    Waro NAKAHARA, Reiko TOKUZEN
    1968 Volume 59 Issue 6 Pages 541-542
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The sarcoma discussed in the first paper of this series (59(6) p. 537) was found to have no inhibiting effect on the Friend virus in vitro and that the presence of growing sarcoma does not suppress the development of the characteristic splenomegaly in mice inoculated with the Friend virus, supporting the contention that the early association of the virus with the sarcoma was merely fortuitous.
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  • Reiko YANAI, Hiroshi NAGASAWA
    1968 Volume 59 Issue 6 Pages 543-546
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The anterior pituitary prolactin and growth hormone levels in rats bearing mammary tumor induced by 7, 12-dimethylbenz[a]anthracene were significantly lower than those in the control. The pituitary and ovary were larger and the adrenal was smaller in the former than in the latter.
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  • Yasuyuki AKAMATSU, Ryoichi IKEGAMI, Koji WATANABE
    1968 Volume 59 Issue 6 Pages 547-549
    Published: December 31, 1968
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    A substrain of C3HeB was established in this institute. This milk agent-flee but susceptible substrain developed spontaneous tumors, i.e. mammary tumors (2.1%), pulmonary adenomas (3.1%), abdominal lymphomas (6.2%), and ovarian cysts (2.1%) in females, and hepatomas (14.3%) and abdominal lymphomas (4.0%) in males. This subline was susceptible to the milk agent but did not pass it on to the offsprings.
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