GANN Japanese Journal of Cancer Research
Print ISSN : 0016-450X
Volume 62, Issue 5
Displaying 1-17 of 17 articles from this issue
  • Keiichi SUEMASU, Keiichi WATANABE, Shichiro ISHIKAWA
    1971 Volume 62 Issue 5 Pages 331-336_1
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    1) Pulmonary metastasis produced after intravenous inoculation of transplantable pulmonary carcinoma of the rat was inhibited by treatment of the cells with dextran sulfate prior to inoculation.
    2) No cytocidal effect of dextran sulfate on the tumor cells was demonstrated by culturing them in the medium containing dextran sulfate.
    3) Dextran sulfate promoted electrophoretic mobility of the tumor cells as well as cultured endothelium-like cells obtained from the rat aorta.
    These results suggest that dextran sulfate binds with the surface of tumor cells and cultured endothelium-like cells whereby electrostatic repulsion, induced by their increased surface negativity, disturbs adhesion between the two in vivo. It is possible that lodgement of tumor cells in the capillaries is inhibited by dextran sulfate and thus the agent contributes to the reduction of experimental pulmonary metastasis.
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  • Jun TAKEUCHI
    1971 Volume 62 Issue 5 Pages 337-342
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Examinations were made on the effect of chondroitinsulfate on the growth of Ehrlich ascites tumor after in vitro incubation and a relation between the growth-promoting activity of chondroitinsulfate and suppression of the growth of rechallenged tumor cells.
    The tumor cells were suspended in 2% chondroitinsulfate solution or saline and were kept at 37° for 30min. One ml of 2% chondroitinsulfate solution was injected into the subcutaneous space on back of mice, immediately followed by inoculation of tumor cell suspension. It was observed that chondroitinsulfate acted as a growth-stimulating agent in vivo and also in vitro.
    One ml of 2% chondroitinsulfate was injected subcutaneously into the back of mice, immediately followed by rechallenge of tumor cells into the same site, on the 1st to 14th day after the 1st challenge into the buttock of mice. On the 8th day after rechallenge, average tumor weight in chondroitinsulfate-treated groups was compared with that in control groups. It was observed that chondroitinsulfate accelerated the growth of tumor both in the case of rechallenge and in challenge only, indicating that chondroitinsulfate counteracts the suppression of the growth of rechallenged tumor cells by the host. Stimulating activity of chondroitinsulfate on the growth of tumor was discussed.
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  • Shigeo SUZUKI, Masuko SUZUKI, Tatsuji MATSUMOTO, Yoshio OKAWA
    1971 Volume 62 Issue 5 Pages 343-352
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Significant growth inhibition on sarcoma-180 solid tumor was demonstrated in the mice which received the regional lymph node cells from mice implanted with the tumor cells and injected with yeast polysaccharide, glucan or mannan. The degree of inhibition was very weak in the mice which received regional lymph node cells from the mice implanted with sarcoma-180 tumor cells without the polysaccharide treatment. On the other hand, the mice implanted with spleen cells from the mice implanted with sarcoma-180 tumor cells regardless of the administration of yeast polysaccharides were found to be significantly resistant to the growth of implanted tumor.
    When sheep erythrocytes were administered intravenously into the mice, which were implanted with sarcoma-180 solid tumor and injected with yeast glucan or mannan, the number of antibody-forming cells in the spleen detected by the plaqueformation technique was significantly larger than that in untreated mice.
    The conclusion based on the above findings is that both polysaccharides, glucan and mannan, are similar in their behavior to stimulate the antibody response against foreign substances such as sheep erythrocytes and implanted tumor cells.
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  • Ryo FUKUNISHI, Kenshi WATANABE, Shin-ichi TERASHI, Kiyotaka KAWAJI
    1971 Volume 62 Issue 5 Pages 353-358_1
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Oral administration of cycasin in various dosage schedules resulted in tumor induction in different organs of the rats. In one group, a small dose of cycasin was administered continuously. A considerable number of mammary cancer was detected in Sprague-Dawley female rats and interstitial cell tumors of the testis in ACI males. Acceleration of spontaneous tumor induction was suspected. Multiple pulse doses and a large single dose of cycasin were administered to other groups. Tumors of the intestine and kidney were observed in high incidence in these experiments. Cirrhosis of the liver was found exclusively in the pulse-dose group.
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  • Nobuyuki ITO, Sachio MAKIURA, Yoshiteru YOKOTA, Yoshiyuki KAMAMOTO, Yo ...
    1971 Volume 62 Issue 5 Pages 359-365_2
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The effect of unilateral ureter ligation on the development of tumors in the renal pelvis, ureter, and urinary bladder of male Wistar strain rats treated with N-butyl-N-(4-hydroxybutyl)nitosoamine was investigated. The activities of β-glucuronidase in homogenates of liver, kidney, and bladder of rats after unilateral ureter ligation were also examined. The incidence of kidney and ureter tumors in rats treated with N-butyl-N-(4-hydroxybutyl)nitrosoamine was much higher after ligation of one ureter. The present reuslts showed that unilateral ligation of the ureter was a good method to induce transitional cell carcinomas in the kidney and ureter of rats treated with N-butyl-N-(4-hydroxybutyl)nitrosoamine. Hyperplasia of the epithelium of the renal pelvis and ureter was occasionally seen in carcinogen-treated rats. It was suggested that the hyperplastic changes were precursors of transitional cell carcinoma of the renal pelvis and ureter in rats receiving N-butyl-N-(4-hydroxybutyl)nitrosoamine. The activity of β-glucuronidase was raised in tumors of the urinary bladder but the activity of this enzyme in liver and kidney tissues was not influenced by the administration of N-butyl-N-(4-hydroxybutyl)nitrosoamine.
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  • Takeshi OGURA, Fumio HIRAO, Eiro TSUBURA, Yuichi YAMAMURA
    1971 Volume 62 Issue 5 Pages 367-372_4
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The distribution of 131I-labeled fibrinogen was studied by macro- and microautoradiography in 10 rabbits bearing lung cancer induced by intrabronchial infusion of 3-methylcholanthrene and 4-nitroquinoline 1-oxide or 3-methylcholanthrene alone.
    The localization of fibrinogen was invariably observed in the non-cancerous pulmonary tissues around and adjacent to the induced cancer tissues, irrespective of histological type of cancer tissues. However, the localization of fibrinogen in cancer tissue itself was observed in a few rabbits and it was observed limitedly in the interstitial spaces and desquamated cells.
    The extrapulmonary tumors by the transpleural invasion of the induced cancer showed the most preferential localization of fibrinogen.
    From these results, it may be concluded that fibrinogen localization in and around the cancer tissue of rabbits resulted from deposition in cancer tissue itself and an interaction of tumor tissues with the host. It seems reasonable to postulate that similar events would occur in human patients with lung cancer because no distinct difference lies in macro- and micro-scopic findings of lung cancer between humans and rabbits.
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  • Eiji ISHIKAWA, Yuzo MATSUOKA, Masami SUDA
    1971 Volume 62 Issue 5 Pages 373-380
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    About 25ml of blood from tumor-bearing rats, which had been transplanted with Walker carcinosarcoma 13 to 18 days before sacrifice, or of normal blood was infused into the juglar vein of normal rats, while removing the same volume of blood from the carotid artey. The incorporation into protein of L-leucine[U-14C], injected intraperitoneally 1hr after blood exchange, was inhibited with tumor-bearing blood; 73% in plasma, about 40% in liver, kidney, and spleen, 33% in bone marrow, and not significantly in pancreas, small intestine, and skeletal muscle, and the inhibition was reversed gradually and completely 17hr after blood exchange. In the incorporation of L-leucine[4, 5-3H] into protein of liver slices from rats which had been subjected to blood exchange 17hr before sacrifice, no difference was observed between tumorbearing and normal blood, while the isotope incorporation into protein was stimulated in liver slices of tumor-bearing rats as compared with that of normal rats, suggesting that such an inhibitory factor(s) may be inactivated in normal rats and that the synthesis of liver protein is stimulated in response to its continuous existence. The inhibitory effect on the isotope incorporation into plasma proteins was obtained with the cellular fraction but not with the plasma fraction of tumor-bearing blood.
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  • Tsutomu YAMAMOTO, Hiroo KATO
    1971 Volume 62 Issue 5 Pages 381-387
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Lauren's histological classification of gastric carcinoma based on the presence of either intestinal or diffuse type was applied to autopsy cases in the fixed population sample of Hiroshima and Nagasaki for 1961-1969. The intestinal type was chiefly seen in older persons and the diffuse type was more frequent in younger persons, especially in women. The ratio of the intestinal type to the diffuse type in Japan was compared with this ratio for high or low risk areas in other countries. The trend of this ratio was similar to that in other cities. There was no statistically significant relation between the occurrence of either type of gastric carcinoma and radiation exposure.
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  • Toshiharu MATSUSHIMA, Tosihide H. YOSIDA
    1971 Volume 62 Issue 5 Pages 389-394_3
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    In the Yoshida rat sarcoma, two peculiar marker chromosomes in metaphase cells were recently observed in several stocks maintained in Japan. These two elements, one a large LS-marker and the other a small SS-marker, were characterized by an abnormally elongated part of the chromosome resembling a long satellite. The length of the quasi-satellite was different in different cells. Similar LS- and SS-markers were found in stocks maintained in Sasaki Institute, Tokyo, National Institute of Genetics, Mishima, Gifu Univeristy, Gifu, and Tohoku University, Sendai, but the LS-marker in the stock of Biological Research Laboratories of Takeda Chemical Ind., Ltd., Osaka, had a different shape, as if a small chromosome were united to the end of a long satellite. Frequency of the LS- and SS-markers was markedly different in different stocks. Higher frequency of markers was observed in those at Sasaki Institute, National Institute of Genetics, and Takeda Biological Research Laboratories, slightly lower in those at Gifu University, and very low in that at Tohoku University. The SS- and LS-markers at times had the appearance of satellite association. From the record of distribution of the tumor stocks, it is suggested that mutant cells with the peculiar markers occurred originally in Sasaki Institute, and then were propagated in several institutes in Japan. In Takeda's stock, translocation seems to have occurred secondarily in the LS-marker.
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  • Masanori KURATSUNE, Seishi KOHCHI, Akio HORIE, Masahiro NISHIZUMI
    1971 Volume 62 Issue 5 Pages 395-405
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    In order to experimentally clarify the established epidemiological association between drinking and esophagus cancer, whiskies, Japanese Sake, sherry, ethanolic solutions of the evaporation residues of these beverages, and aqueous solutions of ethanol of various concentrations were tested for carcinogenicity in mice or rats by skin painting, forced drinking, and subcutaneous injection. Except those of probably spontaneous origin, no malignant tumors ascribable to such chronic treatments were formed. A tumor-promoting activity similar to the one of croton oil, however, was noted for Sake and its distillation residues when tested in mice following the common procedures using 7, 12-dimethylbenz[a]anthracene as an initiator. Whisky seemed to possess a tumor-promoting activity but the evidences obtained were not certain.
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  • Hayase SHISA, Yasuaki NISHIZUKA
    1971 Volume 62 Issue 5 Pages 407-412_1
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    High yield of leukemia induction is consistently observed in Swiss mice injected with a single dose of lipid emulsion of 7, 12-dimethylbenz[a]anthracene (DMBA) at both neonatal and adult ages. However, the prevalent types of leukemias thus evoked may differ depending on the age of animals when DMBA is given. Thymic lymphocytic leukemia is most frequent when the chemical is given at neonatal age, whereas undifferentiated cell leukemia without thymus involvement is common when the chemical is injected at adult age. Frequent occurrence of thymic lymphocytic leukemia is also observed in adult mice by injection of DMBA either at postpartum period or after a single injection of cortisone acetate. Thymuses in these conditioned animals showed prompt decrease in weight due to marked depletion of lymphocyte population which was followed by rapid increase in weight due to lymphocyte proliferation. Thymuses in such situations may be comparable to the thymus at the neonatal age.
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  • Isao MIYOSHI, Harumi HASEGAWA, Teruhiko TSUBOTA, Hiroshi MASUJI, Kiyos ...
    1971 Volume 62 Issue 5 Pages 413-417_2
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Twelve lymphoblastoid cell lines have been established from peripheral blood and lymph node of patients with malignant and benign diseases. The sequential morphological changes of peripheral leucocyte cultures are, first, the emergence of macrophages, then intimate interactions between the macrophages and surviving lymphocytes, and eventual blastogenic transformation of the lymphocytes.
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  • Chikayoshi NAGATA, Yusaku TAGASHIRA, Motoko INOMATA, Masahiki KODAMA
    1971 Volume 62 Issue 5 Pages 419-422
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    5-Hydroxy-3, 4-benzopyrene which has been reported to be non-carcinogenic was found to be weakly carcinogenic. This result is discussed in relation to our previous finding that 5-Hydroxy-3, 4-benzopyrene was easily converted to 5-phenoxy radical and this free radical was produced from 3, 4-benzopyrene enzymically when incubated with skin or liver sap.
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  • Tsuneo BABA, Ken AOKI, Mariko KAKU, Noritaka KIMURA
    1971 Volume 62 Issue 5 Pages 423-426
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Much higher population density, total cell number, and surface-attached cell number per flask than those in controls were obtained by N-acetylhexosamines added in the cultures of rat fibroblast, HeLa-S3, and 3T3 cells. Stimulation of hyaluronic acid synthesis in these lines by the aminosugars was also suggested.
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  • Hisashi OHTSUKI, Minoru AMANO
    1971 Volume 62 Issue 5 Pages 427-430_2
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The nuclei of AH-130 hepatoma cells were isolated with a nonionic surface-active agent. Nonidet P-40. The isolated nuclei were enclosed by only inner membrane and showed 0.64 as the ratio of RNA/DNA. Nucleotide compositions of nuclear RNA were not much different from those of nuclei isolated without the surfactant.
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  • Hiroshi NAGASAKI, Shosuke TOMII, Tomoichi MEGA, Masco MARUGAMI, Nobuyu ...
    1971 Volume 62 Issue 5 Pages 431
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The chronic toxicity of benzene hexachloride in male dd mice was examined. Seventy-four mice were divided into 4 groups of Group 1 660.0ppm, Group 2 66.0ppm, Group 3 6.6ppm, Group 4 control. The tumors developed in the liver of all 20 rats in Group 1. No remarkable changes were seen in the livers of mice in Groups 2, 3, and 4.
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  • Tomiko TANAKA, Tohru TOKUNAGA
    1971 Volume 62 Issue 5 Pages 433-434
    Published: October 31, 1971
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Intradermal inoculation of a mixture of living tumor cells and live Mycobacterium bovis (BCG) into normal syngeneic mice resulted in suppression of tumor growth and development of specific tumor immunity. Tumors used were three syngeneic and four allogeneic tumors.
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