GANN Japanese Journal of Cancer Research
Print ISSN : 0016-450X
Volume 63, Issue 1
Displaying 1-19 of 19 articles from this issue
  • Mitsutaro AKAO, Keiko KURODA, Komei MIYAKI
    1972 Volume 63 Issue 1 Pages 1-10
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The present study was undertaken to determine whether the decrease of rat liver RNA by the administration of 4-(dimethylamino)azobenzene (DAB) is brought by the acceleration of the degradation of RNA or by the suppression of RNA synthesis. The half life of 14C-RNA, used as the measure of RNA degradation, was not shortened by the administration of DAB, while RNA polymerase activity of the isolated liver nuclei activated by Mn2+ and (NH4)2SO4 was significantly suppressed. RNA polymerase activity activated by Mg2+ was rather stimulated. A suggestion was also made that DAB prevents newly synthesized RNA from being fixed as the cellular constituents.
    A similar observation on liver RNA metabolism was made in the animals in which hepatic carcinogenesis by DAB was inhibited by the concurrent administration of either 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide or 2-amino-5-[2-(5-vitro-2-furyl)-1-(2-furyl)vinyl-1-]-1, 3, 4-oxadiazole. Liver RNA/DNA ratio and Mn2+-(NH4)2SO4-activated RNA polymerase activity were not reduced in these animals. Both RNA polymerase activities and RNA/DNA ratio in the isolated liver nuclei were considerably increased by the concurrent administration of a nitrofuran.
    It is concluded that the decrease of liver RNA by the administration of DAB is due to the suppression of the processes of RNA formation, and that the nitrofurans antagonize the action of DAB on liver RNA by increasing the RNA polymerase activity.
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  • Takao IWAGUCHI, Yoshio SAKURAI
    1972 Volume 63 Issue 1 Pages 11-20
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The growth of an ascites hepatoma, AH-13, in the rat was always accompanied by a strong peritoneal bleeding. Rats given a lyophilized powder of tumor cells intraperitoneally bled and a similar bleeding was also observed on administration of the lyophilized powder of a normal rat liver. The ribosomes or the smooth endoplasmic reticulum of the rat liver had the bleeding activity. Intraperitoneal injection of the smooth endoplasmic reticulum of the liver resulted in the appearance of a large number of macrophages followed by a strong peritoneal bleeding. During the growth of tumor cells in vivo the number of degenerated cells in the ascites should be increased and the ribosomes released from damaged cells play a part in the peritoneal bleeding of the host animals.
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  • Tomomi IWASAKI, Kimimaro DEMPO, Aiko KANEKO, Tamenori ONOE
    1972 Volume 63 Issue 1 Pages 21-30_3
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Fluctuaton in population of cells and the alteration of cell characteristics in liver of rats during the early stage of carcinogenesis by 3'-methyl-4-(dimethylamino)azobenzene (3'-Me-DAB) were studied histologically, ultrastructurally, and biochemically.
    After commencement of 3'-Me-DAB administration at a concentration of 0.06%, the initial change in liver cells was the pronounced degeneration of hepatocytes, and consequently the original hepatocytes gradually disappeared, being replaced by the proliferation of cholangiolar cells (the so-called oval cells) after 2 weeks of ingestion of 3'-Me-DAB. Such quantitative change in cellular population was represented by the decreases in liver weight, glucose-6-phosphatase activity, and content of proteinbound dye. Oval cell proliferation in the periportal area of hepatic lobules reached a maximum in the 4th week occupying about one-half of each lobule and, thereafter, the small hepatocytes transformed from the oval cells appeared in the periportal area. The ultrastructural characteristics of these cells revealed various transitional phases toward hepatocytes. They extended to the central vein of most lobules, increasing in their volume, and by the 11th week, the original hepatocytes were almost completely replaced by these renewed hepatocytes. It seemed that the period of appearance of the renewed small hepatocytes corresponded to that of appearance of α-fetoprotein in the sera.
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  • Aiko KANEKO, Kimimaro DEMPO, Tomomi IWASAKI, Tamenori ONOE
    1972 Volume 63 Issue 1 Pages 31-40
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The activities of glucose-6-phosphatase, aldolase, and alkaline phosphatase were measured in rat liver during the early stage in the feeding of 3'-methyl-4-(dimethylamino) azobenzene. Enzyme activities changed quantitatively and qualitatively according to histological alteration in the cell population.
    The fluctuation of enzyme activities occurred in three stages. The first stage was observed within 3 weeks after dye feeding. Activities of three enzymes decreased markedly, except for a slight increase in Mg2+-insensitive alkaline phosphatase at the second week. The second stage was seen between 4 and 8 weeks with a peak at the 6th week. Enzyme activities were elevated, and the muscle-type aldolase and Mg2+-insensitive alkaline phosphatase increased. In the third stage, after 8 weeks on, the altered patterns of these enzymes were maintained in hepatocyte fractions throughout the precancerous stage. It was suggested that the hepatocytes regenerated during 4-8 weeks acquire different characters from those of the origianl hepatocytes.
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  • Aiko KANEKO, Kimimaro DEMPO, Tamenori ONOE
    1972 Volume 63 Issue 1 Pages 41-48
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The acid phosphatase activity in rat liver during the early stage in the feeding of 3'-methyl-4-(dimethylamino)azobenzene was examined with the liver homogenate, soluble fractions, fractions separated by column chromatography, and hepatocyte suspensions, using β-glycerophosphate and phenyl phosphate as substrates. The isozyme pattern of acid phosphatase was also studied by electrophoresis on cellulose acetate membrane.
    The alteration in acid phosphatase activity showed three phases. The first phase occurred after 1-3 weeks of dye feeding, in which the enzyme activity decreased markedly, The second phase was observed during 4-8 weeks and showed recovery of the activity with a peak in the 6th week. The isozyme pattern of acid phosphatase in the liver and also in the hepatocyte fraction changed in this stage. The third phase appeared after 9 weeks. Through these phases, the ratio of activity with phenyl phosphate to that with β-glycerophosphate was higher than the normal value.
    These changes seem to result from alterations of cell population and character of hepatocytes in liver by ingestion of the carcinogen.
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  • Shiroh IDA, Takeshi SAIRENJI, Yorio HINUMA
    1972 Volume 63 Issue 1 Pages 49-55
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Titration of antibodies against a new non-virion antigen induced by Epstein-Barr (EB) virus (anti-N) in sera from patients with malignant and non-malignant diseases, as well as sera from healthy blood donors was performed by means of an indirect immunofluorescence procedure. The results reaffirmed the unusual frequency (72%) of anti-N in sera from nasopharyngeal carcinoma patients. The incidence of anti-N was also high in sera from patients with acute and chronic lymphatic leukemia (38%) and with malignant lymphomas (17%). In contrast, less than 7% of sera from patients with other neoplasms and with infectious mononucleosis had anti-N. Healthy donors uniformly failed to have positive anti-N (1:10). Forty-four percent of sera with anti-N gave titers of anti-virion (anti-V) in the normal range (_??_1:160), and only 4% of sera with negative anti-N from neoplasm patients had a high titer (_??_1:320) of anti-V. These findings suggest that the detection of anti-N is more specific than the titration of anti-V for examination of the relationship between malignant diseases and EB virus infection.
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  • Kiyoko KASHIWAGI, Takashi TOBE, Tokuhiko HIGASHI, Kotaro WARABIOKA
    1972 Volume 63 Issue 1 Pages 57-62
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Depressed synthesis of liver catalase in rats bearing ascites hepatoma cells (AH-49 WH) was examined and the evidences obtained were (1) lower incorporation of injectded 14C-leucine into liver catalase, (2) decrease in the amount of catalasesynthesizing ribosomes in liver, and (3) impaired synthesis of liver catalase in cell-free system.
    These results strongly support the conclusion from immunochemical assay of catalase that the decrease of hepatic catalase activity in tumor-bearing rats is caused by the depression of its biosynthesis.
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  • Hidehiko ISAKA, Sumiko UMEHARA, Hidematsu HIRAI, Yutaka TSUKADA
    1972 Volume 63 Issue 1 Pages 63-71_4
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Three transplant lines of slow-growing variant cells producing α-fetoprotein, both in vivo and in vitro, were successfully obtained from a clonal cell population of the Yoshida ascites sarcoma cultivated in vitro for more than 5 months with or without mutagen treatment. Cells of these variants had altered karyotypes differing from each other and from the original Yoshida tumor cells. This finding may support the hypothesis on the hepatic cell origin of Yoshida ascites tumor.
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  • Noboru TAKASUGI
    1972 Volume 63 Issue 1 Pages 73-77_2
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Basal cell carcinomas occurred in the epithelium of the vagina transplanted from old ovariectomized mice receiving neonatal estrogen injections into ovariectomized "normal" mice after residence of more than 2 years in the hosts. Neither hyperplasia nor proliferation took place in the epithelium of vaginal transplants if the donor had not been estrogenized during neonatal life. Following serial transfer, the carcinoma metastasized to the mesentery and the pancreas. The possible relation between vaginal carcinogenesis and irreversible proliferation of the vaginal epithelium induced by neonatal treatment with estrogen was discussed.
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  • Yoshiyuki HASHIMOTO, Hiroko OSHIMA, Hidetake YUKI
    1972 Volume 63 Issue 1 Pages 79-86
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Carcinostatic 5-benzyl-3-(1'-anilinoethylidene)pyrolidine-2, 4-dione (TN-16) and 5-(p-hydroxybenzyl)-3-(1'-anilinoethylidene)pyrolidine-2, 4-dione (TN-17), structural analogs of tenuazonic acid, showed a remarkable metaphase-arresting effect on Yoshida sarcoma cells in vitro. Minimum effective concentration of TN-16 to arrest tumor cells at metaphase was 0.1μM, which was one-tenth as active as colchicine.
    In contrast to colchicine, metaphase-arresting effect of TN-16 and TN-17 was expressed only when the compound was present in the culture medium. Thus Yoshida sarcoma cells arrested at metaphase by TN-16 or TN-17 divided within 1hr after release from the compound.
    The fact that the metaphase-arresting action of TN-16 did not compete with colchicine, and colchicine action overcame that of TN-16 suggests that the receptor sites of Yoshida sarcoma cells for the two compounds may be different, or the affinity of TN-16 to the receptor site is weaker than that of colchicine.
    Activities of tumor cells to synthesize nucleic acids and protein were not specifically inhibited by TN-16.
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  • Takao NAGOYA, Yorio HINUMA
    1972 Volume 63 Issue 1 Pages 87-93
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Production of infective Epstein-Barr (EB) virus in the culture medium of a Burkitt lymphoma cell line, P3HR-1, was examined. Determination of the infectious titer of the virus was followed by detection of EB virus-directed new (N) antigen formation in the cells of a clonal line (C-6) derived from the NC-37 cell line and infected with EB virus. The percentages of N antigen-forming cells in these cultures were roughly parallel to the dose of the inoculated virus. The amounts of infective virus produced were higher with incubation of the cells at 33° than that at 37°. The amounts produced were also higher in cultures with a low density of cells than in that with a high density of cells. Culture of P3HR-1 cells with a larger fraction of virion antigen-bearing cells yielded a larger amount of the infective virus. Stability of the infectivity at various test temperatures showed that the virus is most stable at -80°, but was less stable at-20° than that at 4° which was comparable to that at 33°. Infectivity was lost most rapidly at 37°. From these results, ideal culture conditions for production of the virus were proposed and nature of the virus was discussed.
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  • Yuhsi MATUO, Katsuzo NISHIKAWA, Takekazu HORIO
    1972 Volume 63 Issue 1 Pages 95-110
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The in vivo liver catalase-depressing substance present in the water-soluble extract from Rhodamine sarcoma of rats was found to have various molecular weights.
    1) The extract contains a proteolytic enzyme.
    2) By molecular sieve of the extract on Sephadex G-200, the catalase-depressing substance is separated into four fractions according to their molecular weight; F-1 of >200, 000, F-2 of 200, 000-40, 000, F-3 of 40, 000-7, 000, and F-4 of <7, 000. All the fractions other than F-4 show both the catalase-depressing and proteolytic activities.
    3) When the extract is heated at pH 4 and 70° for 15min, the proteolytic activity is mostly lost, whereas the depressing activity is not affected. F-1, F-2, and F-3 obtained from the heated extract show the depressing activity. By repeated heating of F-1 and F-2, the substances present are further segmented into constituents corresponding to F-2, F-3, and F-4. All the resulting F-3's do not change in molecular weight on repeated heating. These F-3's migrate, in polyacrylamide gel electrophoresis, forming a single zone of protein. The molecular weight of F-3's is composed of 80% protein and 20% DNA.
    4) The elemental unit of the catalase-depressing substance seems to have a molecular weight of approximately 12, 000, and its protein moiety is indispensable for the depressing activity. The depressing activity may be exhibited by various complexes different in molecular weight, which are formed beteween the elemental unit and others.
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  • Shoichi NAKASHIMA, Shiroh ONO
    1972 Volume 63 Issue 1 Pages 111-117
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Effect of 4-nitroquinoline 1-oxide and its derivatives on immune response in mice was studied. Intraperitoneal administration of oncogenic 4-nitroquinoline 1-oxide and 4-hydroxyaminoquinoline 1-oxide after antigenic stimulation suppressed the immune response to bacterial α-amylase. The dose dependence of immune suppression with 4-nitroquinoline 1-oxide was different from that with 4-hydroxyaminoquinoline 1-oxide. The immune suppression was most clearly observed in mice treated with 0.1ml of 0.05% suspension once a week, among the experiments with 4-nitroquinoline 1-oxide. Mice injected with 0.1ml of 0.1% suspension of the agent twice a week showed almost the same magnitude of the immune response with that of the control group. On the contrary, treatment with 0.1ml of 0.1% suspension of 4-hydroxyaminoquinoline 1-oxide twice a week caused more clear immune suppression than treatment with 0.1ml of 0.05% suspension of the reagent. Non-oncogenic 4-aminoquinoline 1-oxide had no immunosuppressive effect at any doses tested in the present studies.
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  • Motoko INOMATA, Chikayoshi NAGATA
    1972 Volume 63 Issue 1 Pages 119-130
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    It was found that by photoirradiation a phenoxy radical was produced from 3, 4-benzopyrene in such organic solvents as benzene and carbon disulfide. From the analysis of hyperfine structure of the ESR signal, the radical was identified as 5-phenoxy-3, 4-benzopyrene radical which was previously found to be produced by stirring 3, 4-benzopyrene in aqueous albumin solution or incubating it with liver homogenate. No free radical was produced when the light of wavelength at which no absorbance of 3, 4-benzopyrene exists was irradiated, showing that the energy absorbed by benzopyrene was involved in the process of radical production. Dependency of the radical production on the solvent was remarkable; thus, order of reaction was different for benzene and carbon disulfide, and no ESR signal was observed when irradiated in dioxan, ethanol, or acetone. 3, 4:9, 10-Dibenzopyrene and 1, 2:4, 5-dibenzopyrene, which have the 3, 4-benzopyrene skeleton in the molecule, gave ESR signals characteristic of the phenoxy radical, whereas no signal was observed for anthracene, 2, 3:7, 8-dibenzophenanthrene, and 1, 2-benzanthracene. 5-Phenoxy radical was proved to be an intermediate from 3, 4-benzopyrene to the benzopyrene-quinones.
    In the light of these findings, we proposed a mechanism of phenoxy radical production, taking into account of a participation of the singlet molecular oxygen. Significance of the formation of the phenoxy radical was discussed in relation to the photooxidation and carcinogenesis of 3, 4-benzopyrene.
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  • Yutaka TSUKADA
    1972 Volume 63 Issue 1 Pages 131-133
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    The influence of blood transfusion on hepatocarcinogenesis by 4-(dimethylamino)azobenzene (DAB) feeding in rats was investigated. A slight prolongation of DAB carcinogenesis was demonstrated in the group of rats receiving blood transfusion.
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  • Keiji FUJII, Haruo SATO
    1972 Volume 63 Issue 1 Pages 135-137_2
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Spontaneous endocrine tumors in mastomys (6 adenomas, 5 hyperplastic nodules, and 1 cystadenoma of the adrenal cortex, 1 pheochromocytoma of the adrenal medulla, 5 pituitary adenomas, 1 pituitary hyperplastic nodule, and 3 tumors of the female reproductive organs) were observed in 19 out of 158 animals examined.
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  • Yasuaki NISHIZUKA, Yukiko TANAKA, Teruyo SAKAKURA, Akinori KOJIMA
    1972 Volume 63 Issue 1 Pages 139-140
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Ovarian dysgenesis characterized by deletion of follicles and corpora lutea can be induced by neonatal thymectomy in mice. It is demonstrated that development of granulosa cell tumors from the dysgenetic ovaries is obseved in ca. 40% of thymectomized mice after the age of 15 months without any exogeneous carcinogenic stimulation.
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  • Kazuto IKEMOTO, Tadashi YAMAMOTO
    1972 Volume 63 Issue 1 Pages 141-141_1
    Published: February 29, 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
    Rat osteosarcoma was induced by inoculation of murine sarcoma virus (Moloney) into the tibial bone marrow of neonatal rats at a high frequency and in a relatively short period. Radiography revealed largely osteoplastic changes in the metaphyses of tibial bones. These osteosarcomas showed close similarity to the human one, both histologically and radiographically.
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  • 1972 Volume 63 Issue 1 Pages e1
    Published: 1972
    Released on J-STAGE: October 23, 2008
    JOURNAL FREE ACCESS
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