Chagyo Kenkyu Hokoku (Tea Research Journal)
Online ISSN : 1883-941X
Print ISSN : 0366-6190
ISSN-L : 0366-6190
Volume 1985, Issue 61
Displaying 1-9 of 9 articles from this issue
  • Tadahiro NAGATA, Shinsuke SAKAI
    1985 Volume 1985 Issue 61 Pages 1-6
    Published: June 01, 1985
    Released on J-STAGE: July 31, 2009
    JOURNAL FREE ACCESS
    A method for the analysis of abscisic acid (ABA) in tea leaves was investigated by gas chromatography-mass spectrometry. The procedure based on Yoshida et al. was adopted, as follows. The fresh leaves were extracted with methanol and filtrated. After removal of methanol in vacuo, the residue was dissolved in water and adjusted to pH 3, then extracted with chloroform. The residue obtained after evaporation of chloroform was passed through the Sep-pack silica column. After the addition of ethyl stearate as an internal standard, the ABA fraction was methylated with diazomethan and chromatographed. Separation of compounds was achieved employing a temperature programme on a 1.5m×3mm I.D. 1% XE60 column. ABA-Me was detected by selective ion monitoring of m/z 261 (MH-H2O) in CIMS (iso-buthan). Because selective ion monitoring of the base peak of ABA-Me in EIMS were not sufficient to detect ABA in tea leaves.
    Using the method as described above, the ABA content of tea leaves sampled from three positions on the stem during the 1st and 2nd crop season. In the case of 1st crop season, the ABA content of new leaves was higher than that of old leaves until early June. However, the former was equivalent to the latter after middle June due to decrease in the former. The ABA contents of overwintered leaves from two different positions on the stem did not showed significant difference. In the case of 2nd crop season, the change in the content of ABA in new leaves was approximately similar to that in old leaves
    Download PDF (279K)
  • Masaomi ONIKI, Etsuji HAMAYA, Yasuo ANDO
    1985 Volume 1985 Issue 61 Pages 7-11
    Published: June 01, 1985
    Released on J-STAGE: July 31, 2009
    JOURNAL FREE ACCESS
    To investigate occurrence of resistant strains of the tea anthracnose fungus Gloeosporium theaesinensis against benzimidazole fungicides (Topsin M, Benlate), tea laeves affected by the disease were collected from 17 major tea producing prefectures in Japan. An isolate from each diseased leaf was examined on PDA media containing thiophanate-metyl (Topsin M) in concentration of 0, 1 and 100 ppm. The minimum inhibitory concentration (MIC) of several isolates was also examined.
    In 83 tea fields out of 145 tested the resistant strains were detected, although most of them were intermediate-resistant (1 ppm<inhibition<100 ppm). The highly resistant ones (100<inhibition) were obtained from only 5 fields. Resistant strain ratio of isolates from each prefecture ranged within 3.1-43.9% and the average was 16.2%.
    Download PDF (304K)
  • Mitsuaki KARASUYAMA, Tadakatsu YONEYAMA, Hironobu KOBAYASHI
    1985 Volume 1985 Issue 61 Pages 12-19
    Published: June 01, 1985
    Released on J-STAGE: July 31, 2009
    JOURNAL FREE ACCESS
    The effect of soil nitrogen upon the uptake of fertilizer nitrogen by tea plants was studied by using 15N-manure.
    One-year-old plants were transplanted into pots containing three soils of different nitrogen contents in May. After growing for 6 weeks in a green house 15N ammonium sulphate (10.3 atom %) at the rates of 100, 200, and 400 mg N per pot together with 0.9 g of potassium phosphate (monobasic) was supplied to tea plants.
    These plants were grown for additional 5 weeks (untill their new shoots develope) and then digged out. The plants were divided into new shoots, matured leaves, trunk and branches, and roots, and subjected to chemical and isotope analyses after airdrying, weighing and pulverizing. Soil samples were also taken for chemical determination.
    The results obtained were as follows ; With increase in total nitrogen content of soils, dry weights and total nitrogen contents increased considerably in new shoots and whole plnat parts. Therefore, it was shown that the nitrogen uptake by the plants from both of the fertilizer and soil increased greater in soil of high nitrogen content than in soil of low nitrogen content. However, the contribution of fertilized N to the total nitrogen content of the plant was decreased with increase in total nitrogen content of soil.
    These results suggested that the increase in nitrogen uptake by the plants in soils of high nitrogen content might be due to the increase in nutrient absorption activity, which could be improved by absorption of soil nitrogen.
    Download PDF (488K)
  • Hirotsugu TAKAYANAGI, Toyomasa ANAN, Kenjro IKEGAYA, Muneyuki NAKAGAWA
    1985 Volume 1985 Issue 61 Pages 20-25
    Published: June 01, 1985
    Released on J-STAGE: July 31, 2009
    JOURNAL FREE ACCESS
    The chemical constituents of tea shoots on the dferent stages of growth as to the first and the third crop were determined. During the developmnt of tea shoots the contents of total nitrogen, tannin, caffeine, soluble nitrogen, amino acids decreased.
    On the end of the first flush, the contents of theanine, arginine and serine decressed remarkablg among amino acids and amides.
    Younger shoots were rich in catechin gallates and poor in (-)-epigallocatechin than more developed ones.
    The contents of phosphorus, potassium, zinc and copper were rich in the young leaves, while those of calcium, manganes and alminum were rich in the matured leaves.
    Download PDF (373K)
  • Hitoshi YOSHITOMI
    1985 Volume 1985 Issue 61 Pages 26-35
    Published: June 01, 1985
    Released on J-STAGE: July 31, 2009
    JOURNAL FREE ACCESS
    The equilibrium moisture content which is one of the physical properties of tea shoots was measured for leaves and stems. The results were summarized as follows.
    1. The equilibrium moisture content of tea stems was higher than that of leaves, and its difference increased when the atmospheric humidiy increased.
    2. The equilibrium moisture content also depended upon temperature, and became lower as the temperature rised on the same humidity.
    3. The hysteresis which gave higher moisture contents during desorption than those during adsorption in the epuilibrium moisture content was found, and its width decreased'when the temperature rised.
    4. Halsey equation which was shown as follows was most successfully applied to the measured values after the examination of the several equations.
    lnφ=-a/Mgb
    (See table 6 for the coefficient a and b)
    5. The following equation was recommended as the equilibrium moisture content equation
    which contained a term of temperature.
    lnφ=-a/ebTMC/T
    (See table 8 for the coefficient a, b and c)
    Download PDF (568K)
  • Norio NAKADA, Toshio HARA
    1985 Volume 1985 Issue 61 Pages 36-39
    Published: June 01, 1985
    Released on J-STAGE: July 31, 2009
    JOURNAL FREE ACCESS
  • 1985 Volume 1985 Issue 61 Pages 46-48
    Published: June 01, 1985
    Released on J-STAGE: July 31, 2009
    JOURNAL FREE ACCESS
    Download PDF (266K)
  • 1985 Volume 1985 Issue 61 Pages 49-62
    Published: June 01, 1985
    Released on J-STAGE: July 31, 2009
    JOURNAL FREE ACCESS
    Download PDF (1050K)
  • 1985 Volume 1985 Issue 61 Pages 62-76
    Published: June 01, 1985
    Released on J-STAGE: July 31, 2009
    JOURNAL FREE ACCESS
    Download PDF (1101K)
feedback
Top