The effects of four different auxins, that isindolebutyric acid (IBA), napthaleneacetic acid (NAA), indoleacetic acid (IAA) and 2, 4 -dichlorophenoxy acetic acid (2, 4-D), on callus induction and differentiation of root from stem segment culture of Camellia sinensis (L.) 0. Kuntze were investigated.
(1) The activities of four different auxins for callus induction, judging from both of the callus induction rate and the callus size, were higher in order of 2, 4-D > IBA > NAA > IAA in the case of low concentration (0.001-1.0 mg/l), while of NAA = IBA> IAA > 2, 4-D in the case of high concentration (more than 10.0mg/l) .
(2) In case of root differentiation, judging from both of the differentiation rate and number of root formed, auxin's effects were higher in order of IBA≥NAA > IAA > 2, 4-D. Root differentiation rate was about 80% or the application of the optimum concentration of IBA and NAA, however scarecely observed in the case of 2, 4-D.
(3) When the both stem segments of cv. Yabukita and cv. Fujimidori were incubated with different strength of NAA, the callus induction rate was higher in Fujimidori than in Yabukita in broad range of NAA concentration tested. However root differentiation in Yabukita was better than in Fujimidori in broad range of NAA concentration, in spite of the optimum concentration of NAA was different in both cultivars.
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