With the rooted cutting irradiated in the incubator at different light intensities (PPFD:Photosynthetic Photon Flux Density;0,2,7,70,1300μmolm
-2s
-1), changes in leaf color and the ingredients of the new shoots were investigated. For three weeks from the two-leaf stage of the first crop of tea, the changes in PPFD, leaf color and the ingredients of the new shoots were investigated while directly covered at different shading rates (0%, 85%, 98%, 100%).
PPFD 70μmolm
-2s
-1 made the leaf color dark green, PPFD 7~2μmolm
-2s
-1 made it a lighter green, and PPFD 0μmolm
-2s
-1 etiolated it to white. In the field test, 85% shading made the leaf color dark green, 98% shading made it a lighter green, while 100% shading etiolated it to white.
In the daytime, as the shading rate increased, PPFD decreased; 930μmolm
-2s
-1 under open air, 112μmolm
-2s
-1 under 85%, 7μmolm
-2s
-1 under 98%, and 0μmolm
-2s
-1 under 100%. PPFD on a rainy day decreased to 10% on a fine day, and was at the same level as it was when under 85% shading on a fine day. PPFD under 85% shading on a rainy day was at the same level as it was when under 98% shading on a fine day.
The content of amino acids in the new shoot increased as PPFD lowered and the rate of shading rose. Particularly, the amino acids in shoots with 100% shading increased more than double, as compared with the starting time of their covering. Arginine increased about 3 times, serine about 4 times, and asparagine about 50 times.
The content of catechin, in the new shoot was higher when it was under PPFD1300μmolm
-2s
-1 and 0μmolm
-2s
-1 than under PPFD70μmolm
-2s
-1. The content of caffeine increased as PPFD was lowered and the rate of shading rose.
In conclusion, it was found that decreased PPFD levels makes the leaf color of new shoots dark green. PPFD7~2μmolm
-2s
-1 makes it a lighter green, PPFD0μmolm
-2s
-1 etiolates it to white, and the content of amino acids in new shoots increases remarkably.
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