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HIROSHI FUJITA
1969 Volume 17 Issue 6 Pages
933-937
Published: July 25, 1969
Released on J-STAGE: September 24, 2010
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1) Concentration of bleomycin in the blood, urine, and tissues from cancer patients or experimental animals was estimated by means of bioassay method (thin plate-cup method using
Bacillus subtilis, ATCC 6633 spores as the test organisms).
2) The maximum blood leve l was 3. 3 mcg/ml in the patients received intravenously 15 mg of the drug, and was 0. 97 mcg/ml in those received intramuscularly.
3) Relative high level of bleomycin was det ected in the kidney, lung, liver, skin and spleen in animal experiments (mice).
4) The drug le vel in the tumor was higher than that in the adjacent normal tissues of cancer patients. In tumor bearing mice, relative high or moderate level was obtained in the ascitic fluid and the solid tumor tissues, but very low in the ascitic tumor cells.
5) The drug was recovered at the ratio of 38. 4 % wit hin 24 hours in the urine of the patients received intravenously 15 mg of the drug.
6) The effect of inactivation or adsorption of the drug by various tissue homogenates from mice was very slight as comparing with other anti-cancer drugs.
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HIROSHI AKASAKA, KAZUO IMAMURA, YOSHIO KAI, YUKITAKA MARUYAMA, TSUNEO ...
1969 Volume 17 Issue 6 Pages
938-947
Published: July 25, 1969
Released on J-STAGE: September 24, 2010
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Bleomycin has been proved to be useful in our 3 cases of squamous cell carcinoma of penis. As for its side effects, anorexia with nausea and vomiting in one case and deformity of nails and paronychia in another case have been noted. No side effects have been observed in the last case treated with copper free bleomycin.
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YOSHIHIRO ODAKURA
1969 Volume 17 Issue 6 Pages
948-955
Published: July 25, 1969
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As a part of experimental studies on the combination effect of acetyl-spiramycin and chloramphenicol, the absorption, urinary excretion, metabolism and therapeutic effect on infected mice by combined use of both substances were examined in comparison with those by the single use of respective substances. As the result, there was not so significant difference as to the absorption, excretion and metabolism between the combined use and the single use, while there was found some combination effect of two substances on mice infected with
Pneumococcus, and the synergistic action was more marked on mice infected with
E. conli.
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GEN-ICHI TOKUDA, MITSUO YUASA
1969 Volume 17 Issue 6 Pages
956-960
Published: July 25, 1969
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After the radical operation of uterine cancer, there often occurred the urinary infection.
These infections were perhaps avoided partially by operation method and prophylactic proc edure. But according to the specifity of operation, the damage of urinary tracts could not be avoided totally. These urinary infection by resistant Gram negative bacilli made the treatment difficult. The functional disorder or urinary tracts by chemotherapy was also accompanied. Recurrence and reinfection occurred frequently. The long lasting observation will need for precise examination.
Relationship between number of bacilli and urinary retention w as investigated. And it became clear that the relationship was very close. Decrease of bladder capacity, hyperemia and edema of mucous membrane, deformation of trigonum and ureteral orifice, were noted. These changes of urinary tracts promoted the incidence of urinary infection.
Clinical observation also was disc ussed.
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The Nature of Antivirin, especially its Resistance against Heating and Proteolytic Enzyme-digestion
YOSHIKO SETO, HISAKO TONEGI, SHIGESHI TOYOSHIMA
1969 Volume 17 Issue 6 Pages
961-965
Published: July 25, 1969
Released on J-STAGE: September 24, 2010
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A highly sensitive Antivirin testing system was obtained by measuring the intracellular viral amounts in treated and control cultures at 4 and 6 hours after infection. The resistance against heatingand proteolytic enzyme-digestion was confirmed to be a characteristic of Antivirin.
From the experiments of Gel-filtration using Sephadex G-200, the three active molecules, over 30 X 10
4, 10-11 x10
4 and 4-5x10
4 in molecular weight, of Antivirin were found. 62% Ammonium sulfatesaturated fraction also was 4. 4x10
4in molecular weight, and then it was not inactivated by heatingand trypsin-digestion. When Antivirin was filtered on the column of Sephadex G-200 after trypsin-digestion, the total activity of Antivirin was remained, while any of the three active molecules described above was not detected.
From these results, it may be suggested that Antivirin is about 5 x 104 in molecular weight in the native status, but the active element is smaller one.
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The Effect of Antivirin on Poliovirus Ribonucleic Acid and Protein Synthesis in Infected Cells
OSHIKO SETO, HISAKO TONEGI, SHIGESHI TOYOSHIMA
1969 Volume 17 Issue 6 Pages
966-969
Published: July 25, 1969
Released on J-STAGE: September 24, 2010
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Antivirin depresses the development of infectious poliovirus particles, the incorporation of H
3-uridine into viral specific RNA and the formation of infectious RNA in the early time of the multiplicationstep, even in the presence of actinomycin D.
Antivirin, however, does not depress the incorporation of H
3-uridine into cellular RNA. The inhibitory effect of A antivirin on the incorporation of H
3-valin into viral specific protein is lower than that on viral specific RNA-synthesis.
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Antivirin-E, an Antiviral Substance found in Ascites of Tumor bearing Mice
HARUHISA FUJITA, SHIGESHI TOYOSHIMA
1969 Volume 17 Issue 6 Pages
970-973
Published: July 25, 1969
Released on J-STAGE: September 24, 2010
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Antivirin-E, an antiviral substance found in ascites of Ehrlich ascites tumor bearing mice, was investigated.
Antivirin-E was inhibitory on the intracellular multiplication of polio (types 1, 2 and 3) and vaccinia viruses. The site of action of antivirin-E was considered to be the inhibition of the replicative step of the intracellular multiplication of susceptible virus. The antiviral activity was inactivated by proteolytic enzyme treatment, but not with both RNase and DNase. Antivirin Fs activity was unstable in the pH range of acidic and alkali sites, and was decreased to 50% by heating at 57°C for 60 minutes.
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Antibacterial Activity in vitro
YASUHIRO MINE, MINORU NISHIDA, SACHIKO GOTO
1969 Volume 17 Issue 6 Pages
974-989
Published: July 25, 1969
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1) When AB-PC and MCI-PC were combined
in vitro, the antibacterial spectrum tended to be extended in comparison with each one of the antibiotics.
2) The antibacterial activities
in vitro ag ainst various bacteria were not remarkably influenced by the rates of combination (2: 1, 1: 1, 1: 2) of both antibiotics.
3) The synergism of AB-PC and MCI-PC
in vitro was demonstrated in various bacteria, but the degrees of synergistic activities varied among the bacteria and strains used.
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(2) Preventive Activity of MCI-PC for Microbial Degradation of AB-PC by Some Pathogenic Bacteria
YASUHIRO MINE, MINORU NISHIDA, SACHIKO GOTO
1969 Volume 17 Issue 6 Pages
979-985
Published: July 25, 1969
Released on J-STAGE: September 24, 2010
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1) The combination of AB-PC with MCI-PC showed excellent bactericidal activity, even at the low concentration (1/20 MIC) which was inactive in both antibiotics alone, against a AB-PC and MCIPC resistant strain of
E. coil isolated from a patient. Then in this case, the microbial degradation of AB-PC by this strain was highly prevented in presence of MCI-PC.
2) The enzymatic degradation of AB-PC by extracellar a nd cell-bound PC-ase, obtained from a ABPC resistant strain (No. 39) of
Staph. aureus, were at same degree prevented in presence of MCI-PC.
3) The 13-lactam hydrolysis of AB-PC by the crude PC-ase from several pathogenic bacteria we re VOL 17 NO. 6 CHEMOTHERAPY 985 prevented in presence of MCI-PC, but the rates of hydrolysis were not constant among these bacteria used.
4) In the experiment in which the β-actam hydrolysis of AB-PC and PC-G by the PC-ase was determined, when inhibitor (MCI-PC) added to the incubation mixture of substrate (AB-PC or PC-G) with the PC-ase at start, a marked difference was found between the rates of β-lactam hydrolysis of AB-PC and PC-G by the cell-bound PC-ase from
Staph. aureus No. 39. Namely, the β-lactam of PC-G was almost hydrolysed with this enzyme (the residual activity; 3%) during incubation, while the β-lactam hydrolysis of AB-PC was remarkably prevented under same condition (the residual activity; 53%).
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In vivo synergism, absorption and excretion
YASUHIRO MINE, MINORU NISHIDA, SACHIKO GOTO
1969 Volume 17 Issue 6 Pages
986-992
Published: July 25, 1969
Released on J-STAGE: September 24, 2010
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The enhanced therapeutic activity in vivo was provided in combination (2: 1 of 1: 1) of AB-PC with MCI-PC for treatment of experimental infection of mice, when challenged with a clinically isol ated strain of
E. coli which was resistant to AB-PC and MCI-PC.
In the treatment of mice infected with
Staph. aure us T-5 which was highly resistant to AB-PC but sensitive to MCI-PC, the similar tendency was also obtained in this combination.
When AB-PC and MCI-PC were administered intramuscularly to ani mals, blood, urinary and biliary levels of both penicillins were not influenced by their combination ratio. Furthermore, both peniillins were detected in the urine from rabbit, administered AB-PC and MIC-PC, bythin layer chromatography and agar-gel electrophoresis.
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IKURO KIMURA, YOSHIAKI MORITANI, TAISUKE ONOSHI, YOSHITOMO NISHIZAKI, ...
1969 Volume 17 Issue 6 Pages
993-996
Published: July 25, 1969
Released on J-STAGE: September 24, 2010
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For the purpose of arresting both stromal connective tissue and parenchymal cancer cells, the combined therapy with fibroblast-inhibiting agents(Chloroquine and Phytonadione), anti-cancer agent(Mitomycin C or Cyclophosphamide) and Prednisolone was studied. The results were as follows.
In experimental studies of metastatic lung tumors of YOSHIDA sarcoma and EHRL ICH ascites cancer, the combined therapy was more effective than each single administration. In clinical application, considerable therapeutic effects of this combined therapy were obtained in various human cancers, particularly in lung cancers and malignant lymphomas. It is very interesting that transient prevention of aggravation was observed in the majority of these cancer patients.
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1969 Volume 17 Issue 6 Pages
997-1002
Published: July 25, 1969
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1969 Volume 17 Issue 6 Pages
1003-1008
Published: July 25, 1969
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1969 Volume 17 Issue 6 Pages
1009-1013
Published: July 25, 1969
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1969 Volume 17 Issue 6 Pages
1014-1019
Published: July 25, 1969
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1969 Volume 17 Issue 6 Pages
1020-1023
Published: July 25, 1969
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1969 Volume 17 Issue 6 Pages
1024-1028
Published: July 25, 1969
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1969 Volume 17 Issue 6 Pages
1029-1033
Published: July 25, 1969
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1969 Volume 17 Issue 6 Pages
1034-1039
Published: July 25, 1969
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1969 Volume 17 Issue 6 Pages
1040-1047
Published: July 25, 1969
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1969 Volume 17 Issue 6 Pages
1048-1054
Published: July 25, 1969
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1969 Volume 17 Issue 6 Pages
1055-1060
Published: July 25, 1969
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1969 Volume 17 Issue 6 Pages
1061-1066
Published: July 25, 1969
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1969 Volume 17 Issue 6 Pages
1067-1074
Published: July 25, 1969
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1969 Volume 17 Issue 6 Pages
1075-1087
Published: July 25, 1969
Released on J-STAGE: September 24, 2010
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