In order to study the influ ences of various antibiotics and antifungal drugs upon the metabolism of
C. aibicans, I have investigated their influences upon the adaptive enzymes of the cells. Using a living cell suspension of
C. alb. (F. D. A. No. 1001) as an enzymic preparation which cultured on Sabouraud-glucose agar media at 37. 5 °C for about 24 hours, I have studied the influen ces of various drugs (final concentration 1-10 mcgfcc) upon adaptive oxidation of substrates by using manometric technique of
WARBURG. 1. Mannose and galactose are adaptively oxidized by
C. alb.
2., Both the respiration and the adaptive oxidation of mannose and galactose are inhibited by 8-hydroxyquinoline. merzonin. 2-methy1-3-merca pto1: 4 -naphthoquinone, aureot hricin, candimycin, eurocidin and trichomycin, but not affected by penicillin G, chlortetracycline, oxytetracycline, chloramphenicol, dihydrostreptom ycin, propylparaben, so called “Dehyroacetic acid” and natrium azide.
3. In the case of using mannose or galactose adapted cells, the oxidation of mannose or galact ose is inhibited by 8-hydroxquinoline, m erzonin, 2-methyl-3-mercapto-1: 4-naphthoquinon e, aureothricin and candimycin, but not affected b y eurocidin and trichomycin. Namely eurocidin a nd trichomycin inhibit the formation of the ad aptive enzymes.
抄録全体を表示