Nihon Chikusan Gakkaiho
Online ISSN : 1880-8255
Print ISSN : 1346-907X
ISSN-L : 1880-8255
Volume 1
Displaying 1-16 of 16 articles from this issue
  • M. SUZUKI, Y. MATSUYAMA, N. HASHIMOTO
    1924 Volume 1 Pages 1-18
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
    JOURNAL FREE ACCESS
    The object of this experiment is to compare the amounts of vitamines in milk and some milk products. White rats were fed with artificial food mixtures free from vitamines, but by adding milk or some milk products to be tested as the source of vitamines, from 10 to 50% of the total diet, and the rate of theirgrowth was observed.
    The chief results obtained may be summarized as follows:
    1) An addition of 30% of condensed milk to the diet was far from sufficient to promote the growth of the animals. Even when 50% of it was added to the diet they could hardly attain 140-150 grams in body weight. But, in addition to above-mentioned, when 2-3% of the commercial "Oryzanin liquid" (manufactured by the Sankyo and Co.) was supplied in each case as the source of vitamine B, the rats begun to grow vigorously and their weights reached over 330 grams.
    2) When fed with condensed milk alone, the rats grew up to 270 grams, and their body weights begun to decrease. But, at this point, the addition of 2-3% of mineral salts (Osborne's or McCollum's salt mixture) and 2-3% of the commercial "Oryzanin liquid" was efficient enough to make them grow over 330 grams again.
    3) When the diet contained more than 20% of condensed Milk, deficiencies of vitamines A and C were not observed, but vitamine B was seemingly most lacking.
    4) In comparing the nutritive value of foreign- and home-made condensed milk no noticable differences could be observed, but the latter seemed rather richer in the content of vitamine B than the former.
    5) "Lactogen" is higher in vitamine B than condensed Milk on account of free from cane sugar and containing little water, but it is poorer in vitamines A and C. This is perhaps due to the destruction of the latter factors by oxidation during the process of drying.
    6) "Darigold" is somewhat richer in vitamine B than "Lactogen, " but lacks almost vitamine A and C. This fact is worth while noticing when they use "Darigold."
    7) Milk does not furnish enough vitamine B unless more than 200cc. of it (30 grams as dry matter) is added to 100 grams of the diet. Though the authors are trying still more experiments upon this point, yet it seems well to justify to support the view of Osborne, i. e. the rats will easily attain normalgrowth if 60% of dry matter of the diet is supplied by milk. It may also seem well to assume that 125cc. of milk correspond to about I gram of dry brewers' yeast or 1cc. of the commercial "Oryzanin liquid" in the content of vitamine B.
    8) The anthors came to the conclusion that the nutritive values of milk and some milk products can be markedly increased by additional supply of vitamine B.
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  • SEITARO ADACHI
    1924 Volume 1 Pages 19-39
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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    The inheritance of coat olour in the horse has already been studied by many investigators who have shown that bays are never produced in the mating of chestnut and black. It is, however, curious enough that in the same mating most of them perceived the production of bays in comparatively high percentage.MÜller and others explained this as the error of observations. Thus it is difficult to explain clearly the inheritance of coat colour in the horse. It is for this reason that the present study was undertaken. The chief results obtained may be summarized as follows:- 1. With regard to the genetic onstitution of bays we can presume four kinds of factors, since bays mated with each other produce four types of segregations in the colour of their offsprings viz. a) bays only, b) bays and chestnuts in the ratio 7: 1, c) bays and blacks in the ratio 7: 1, d) bays, chestnuts and blacks in the ratio 49: 8: 7. 2. Chestnuts mated with each other produce nothing but the same colour in their offsprings. 3. In mating blacks together two types of segregations result in the colour of their offsprings viz. a) blacks only, b) blacks and chestnuts in the ratio 1: 7. Thus it can be shown that there are two kinds in the enetic constitution of blacks. 4. As chestnuts mated with bays produce three, types of segregations in the colour of their offsprings, so we can presume three kinds in the constitution of chestnuts. 5. That there are such several kinds in genetic costitution of each colour is also clearly confnmed by the other possible matings.
    From the above facts we can reach the following conclusion.
    1. All the coloured varieties of horses are characterized by possesing the factor (C) which has already been determined by MÜller. He has shown that chestnuts are produced in the presence of (C) alone. But from the results of the matings above mentioned it can be shown that some chestnuts contain one more factor (A) which probably exhibits no visible effect on chestnuts.
    2. In order to produce the bay colour it is necessary to consider the existence of (C), (B) and (A) together.
    3. (A) seems to inhibit partially the production of black colour which is produced in the presence of (B).
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  • S. SATO.
    1924 Volume 1 Pages 40-49
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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    Having studied in Formosa the effects of castration upon the body form of the Formosan zebu, the author came to the following conclusions.
    1. As has already been observed by some workers, the change in the body form due to castration depends largely upon the period, in which the animal is castrated. A remarkable change in the body form is to be observed in the animal which is castrated in its early life (within 18 months), but the change is less remarkable in the animal castrated after maturation.
    2. The most remarkable feature effected by castration is to be observed in the limbs, namely the extremities of the castrated are always longer than those of controls. Accordingly an increase in the height of the body is noticeable in the castrated, while an decrease in the width, depth and circumference of the breast, in the body length and the ciroumference of the metacarps.
    3. It may generally be observed that the effects of castration appear in those parts of the body where the difference according to sex is obviously presented.
    4. The physiognomy and the type of the animal castrated in its early life has a close resemblance to those of the female, but in the animal castrated after maturation one cannot remark any female type.
    5. The effect of castration is generally remarkable in the animals castrated by crushing the spermatic cord (this is the method by which the zebu is generally castrated in Formosa. The animal is cast down in the ordinary way and the spermatic cord with its sunounding blood vessels is striken over the skin by means of a wooden piece 5-15 times till the cord is thoroughly crushed, the part to be operated being placed upon a wooden plate.), than in those castrated by total extirpation of testicles. In the former the male type always remains.
    As regards the reason why the effect of catration is always less remarkable in the animal castrated by crushing the spermatic cord, the auther is of opinion that the interstitial cells of testicles would remain or rather increase during a certain period after castration and then gradually degenerate, pointing out that further studies should be made in connection with the degeneration of testicles after castration by crushing the spermatic cord with its surrounding blood vessels.
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  • KIYOSHI MASUI, YASUSHIGE TAMURA
    1924 Volume 1 Pages 55-79
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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    From clinical and pathological observations in man it has long been inferred that there are some functional relations between the suprarenal gland and the sex gland. Recently from the results obtained by gonadectomy and the histological observations of the gland in some rodents many investigators have thrown light more clearly upon this subject than hitherto. But these differ so strikingly among different investigators that renewed studies are required, especially, as the histological and cytological evidence of the secretory function of this gland which is the essential point in giving a reliable evidence on this subject, so far as we known, has as yet not been indicated. It is for this reason that the present study was undertaken. The chief results obtained may be summarized as follows:
    1. The size of the suprarenal glands differs strikingly according to sex, the females having considerable larger glands than the males. This difference in size is due to the degree of development of the zona reticularis; in the females this zone develops remarkably, while in the mature males this disappears almost entirely, leaving a few number of cells of this kind behind.
    2. The size of the suprarenals of the females shows a considerable difference according to various periods of oestrous cycle, and this variation in size is due to the structural difference of this gland.
    3. In the mature females the amount of the zona reticularis varies strikingly according to every individual animal; in some females this shows enormous development, while in the others a considerable amount of cells of this zone disappears by fatty degeneration.
    4. At the beginning of the pregnancy the degeneration of the zona reticularis begins and it proceeds more and more until this zone becomes disappeared entirely, while the zona fasciculata increases slightly. Immediately after partrition sometimes the cells of the zona reticularis again appear which immediately disappear by fatty degeneration.
    5. In the very young mice the zona reticularis develops both in the males and females. In the females this zone increases gradually, while in the males it disappears prior to the sexual maturity. In the old females the zone disappears almost entirely, attaining the same structure as that of the males.
    6. The suprarenal glands of castrated males are greater than those of the normal males. This enlargement is not simply due to the increase of every tissue, but depends upon the growth of the zona reticularis, while the medulla diminishes slightly. In the castrated the increase of this zona is so strikingly that its amount becomes almost similar to that of the females.
    7. As the result of gonadectomy in the females, no structural changes occur in the supprarenal glands, the zona reticularis showing the same development as in the mature females.
    8. The cells of the zona reticularis contain as the normal components the mitochondria, the secretory granules, the vacuoles and the lipoid granules. According to each individual cell the amount of these granules is extremely varied. From accurate observation it becomes evident that the vacuoles are formed as the result of liquefaction of the contents of the secretory granules.
    From the facts above mentioned the writere came to the following conclusions:
    1. As regards the structure of the suprarenal glands the results obtained by many investigators differ strikingly even among the rodents. Thus whether the structural difference of the suprarenals between the males and females is peculiar to the mouse or whether this may exist in all the mammals is a signiffcant point which requires further observations.
    2. Judging from the structural changes of the zona reticularis in the females it seems most probable that there are functional relations between this zone and the sex gland of the same.
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  • KOZO SUZUKI, MICHIO SAITO, TETSUSABURO NISHIKAWA
    1924 Volume 1 Pages 80-86
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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    The silkworm chrysalis is mainly used as manure and sometimes it is extracted to make the oil for soap manufacturing, but it is not customary to feed the live stock on chrysalis.
    The object of the present investigation is to determine whether the silkworm chrysalis could be utilized as stock feeding.
    The composition and the digestibility coefficient of the chrysalis are as follws.
    Composition.
    Digestibility coefficient for sheep.
    From the compositions and digestibility coefficient above mentioned it can be considered that the silkworm chrysalis is a valuable supplementary food which the live stock generally prefers to eat.
    But the chrysalis has generally corruptible character and peculiar smell. As to these points further investigations will be made.
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  • MICHIO SAITO
    1924 Volume 1 Pages 87-102
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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    The author has investigated the botanical classification and the chemical composition of uncultivated hay which is generally used as the main fodder for cattle in Japan; the results obtained may be summarized as follows:
    1. The hay consists of about 10 families of plants among which Graminae is found most abundantly
    2. The hay contains a small amount of albumin, globulin and prolamin and a considerable amount of the other kinds of protein.
    3. In the protein the amount of lysin is very small but other diaminoacids such as arginin and histin exist in great quantity.
    4. The hay is considered to be suitable fodder for the growth and maintenance of large animals.
    5. The crude fiber content occupies about one third of the carbohydrate which is the main substance of the hay. The incrusting substance of crude fiber such as lignin and cutin are contained in relatively greater amounts. In order to determine the contents of cellulose two methods (König, Cross-Bevan) were empleyed. From the result of estimation of pentosan, dextrin, sugars and starch, it can be found that some undetermined carbonydrates exist in comparatively large quentities
    6. Lecithin was found to exist in trace.
    7. In mineral substances of the hay a large amount of silica was found. Sulphur of cystine nature occupies about one half of the total sulphur, while phosphorus is almost in inorganic form.
    8. The ratios of some mineral matters in the hay, such as CaO: MgO and CaO: P2O5, were found to be in a fovourable condition for the nutrition.
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  • I. ON THE SOURCE OF POISONOUS HONEY
    Y. TOKUDA, E. SUMITA
    1924 Volume 1 Pages 103-121
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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    The poisoning in man due to the "narcotic honey, " as they call it, which is produced in summer or early in autumn in certain mountainous regions of central and northern parts of Japan has frequently been reported. The pure poisonous honey is light-coloured and has no peculiar odor, but a spoonful of it may give one a peculiar pungent, acrid taste, and if swallowed, it causes coughs, and stimulates the stomach. The severity of poisoning varies somewhat with individuals and the amount taken. The principal symptoms are retching, vomiting, (diarrhea in rare cases), headache, palpitation followed by general depression, relaxation or looseness of voluntary muscles, ataxia when highly intoxicated, weakened heart beat, coldness of extremities, slight spasm, dilatation of pupil, exaggerated kneejerk. The patients may generally recover from the attack in several hours or in a few days; there has been no cases leading to death.
    The minimal fatal dose of the sample from Sado-island in the rabbit, when injected subcutaneously, is 3.5gm. per kgm. of body weight; in the mouse it is 20gm. The principal symptoms in the mouse are restlessness, vomiting action, salivation, paralysis of hind body, projected eyes etc, leading to death.
    The source of the poisonous honey produced in these parts mentioned has remained quite unknown and the bee-keepers there have always been embarrassed. Having studied the chief source of the poison since 1921, it has been proved that the main poisoning substance exists in some composition from the nectar of Tripetaleia paniculata, Sieb. et Zucc., "Hotsutsuji, " which is distributed on the hills or mountains in such localities as Naganoken, Iwateken, Sado-island in Niigataken, etc. The following data will be enough to explain the reason why the plant referred to can be determined as the chief source of the poisoning honey.
    1. The poisonous honey in these localities is produced from summer to the beginning of autumn, in this season the plants also bloom.
    2. The plants are generally found in abundance in the localities where cases of the poisoning often take place.
    3. The nectar is found in the bottom of the flowers; bees are seen visiting them.
    4. The pollens of this plant, trichotomous in shape and about 40μ in diameter, have been found in the samples from Sado-island and Iwateken, but none in other ones. The scarcity of the pollens in the samples is probadly due to the fact that the stamens and pollens of this plant are prematured before the flowers bloom and the bees can visit them without being spoiled by the pollens.
    5. The poisonous honey is produced at intervals in one and the same locality; this fact is in fair agreement with the intermittent flowering of the Tripetaleia-shrubs.
    6. The poisonous substances adsorbed by animal-charcoal are extracted with alcohol in an almost pure form. There are at least two poisonous substances in the honey, crystalline und amorphus. The same substances are also contained in the alcohol extract of flower and of leaves of Tripetaleia, The poisonous nature can not be destroyed by heating at 100°C in an hour.
    7. As no trace of nitrogen is to be found in the poisonus substances of the honey, it cannot be considered that the poisonous property of it originates from some ingredients of such plants as Aconitum. Datura, etc. which bloom in summer and contain alkaloids as poisonous matters.
    It is, however, fortunate that the poisonous honey is produced only in the summer season, and thus beekeepers would collect the honey just. after or before the main honey-flow in spring or autumn, and also that Tripetaleia grows only in the mountainous regions of limited localities, where a few dwellers or migratory beekeepers only maintain their bee-colonies.
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  • T. MOCHIZUKI
    1924 Volume 1 Pages 129-139
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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  • Kozo SUZUNI, ATARU YAZAKI
    1924 Volume 1 Pages 140-144
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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    Soy bean cake was used for the diet of white rats and its vitamine contents, and the nutritive value of its proteins were observed. The results obtained may be summarized as follows.
    1 The proteins of soy bean cake have a high nutritive value. When the diet contains soy bean cake proteins 10%, this diet is adequate for promoting normal growth of the rat.
    2 Soy bean cake contains an adequate amount of vitamin B. When the artificial diet contains 20% of soy bean cake, it supplies sufficient amount of vitamin B.
    3 Soy bean cake is deficient in vitamine A.
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  • KOZO SUZUKI, ATARU YAZAKI
    1924 Volume 1 Pages 145-152
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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    White rats were fed with the commereial wheat bran and the observation were made upon its vitamine contents, the nutritive value of its proteins and the nutritive deficiency of its mineral constituents. The chief results may be summarized as follows.
    (1) The proteins of the wheat bran have a high nutritive value. If the diet contains wheat bran proteins over 10%, such a diet is adequate for promoting normal growth of white rats.
    (2) The wheat bran contains an adequate amount of vitamine B. If the diet contains wheat bran 55%, it supplies a sufficient amount of vitamin B for the growth of white rats.
    (3) The wheat bran is deficient in vitamine A.
    (4) As the wheat bran contains a small amount of calcium, so this is not suitable for the growing animal.
    (5) There are no disturbances for the growth and reproduction of white rats when a sufficient amount of vitamine A and calcium is added to the wheat bran.
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  • K. MASUI, J. HASHIMOTO, I. ONO
    1924 Volume 1 Pages 153-163
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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  • SHIRO ITAGAKI
    1924 Volume 1 Pages 165-225
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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    A few observations as regards the life history in some specieses of the chicken nematode, especially Heterakis perspicillum, have hitherto been published, though there are a number of reports upon the development of Heterakis papillosa.
    During the last three years I have been studying the development of the parasite, and the following results were obtained.
    In culture experiment with the eggs of the parasite, Heterakis perspicillum, I have found that it required 7 days to make embryos completely develop within eggs in the hottest season of summer, the eggs being kept in a shallow layer of physiological salt solution or of 0.2% solution of formalin. At room temperature, however, the embryos have never hatched out from the ova, if the same medium was employed and no mechanical or physical irritation has been given to them.
    It has been found that the egge freshly discharged were still alive, in spite of being exposed to severe coldness out of doors for three months, namely, from January to March, but most of them have been unable to develop to the embryonated eggs. The eggs fully developed, which were kept outdoors in frozen water, have also been found vividly alive after a period of four months.
    In order to determine the resistance of the eggs against dryness I have kept some eggs not fully developed in a desiccator and found that, after a course of 44 days, they were not capable of being developed to a state in which they could infect, and that ripe eggs containing larvae were still alive after a period of 60 days.
    The eggs exposed to a temperature ranging from 50°C to 53°C for five minutes, which were have been found still alive, but at 54°C they were found quite dead. The undeveloped eggs whieh were kept in a culture media free from oxygen and those kept in pitch darkness were not able to develop and died within a few days.
    As is known, the shell of Heterakis egg is thick and have a strong resistance thus providing a marked protection against various chemical aubstances. I have made some experiments with regard to the resistance of the shell, and fonnd that it conld not be destroyed in a few hours by the action of ordinary disinfectants. Of all the disinfectants which I employed for this purpose, lysol and crude cresol were found to be most destructive, for the eggs kept in 0.1% solution of these antiparasitic agents could not survive for a period of 3 days.
    It is worthy of note that Heterakis perspicillum needs not any intermediate host during its development. Studies on a series of artificially infested chickens killed at short intervals showed that a number of the eggs of this worm hatched out in the glandular stomach and the rest of them in the muscular stomach, and moreover that the hatched embryos increaesd considerably in size in the small intestine. It appeared to me also that the larvae required 37 days as minimum period to reach their maturity in the alimentary tract of the host.
    I have made some feeding experiments by giving per os the eggs of Heterakis perspicillum to chickens, and have shown that no larva was to be found in lungs, liver, kidney and thoracic or abdominal cavity. It may be considered from this fact that there is no evidence of any migration of the larvae within the body of the host during their development such as the case of Ascaris larvae. On the basis of this experiment it can be said that the development of this worm is very simple and direct. This sort of development is one of the developing ways of Heterakis perspicillum, and I will call this "the Ist developing way."
    In another series of feeding experiments, it has been found that some of the early-stage larvae which reached the small intestine penetrated the mucosa of the domicile through the Liverkuehn's glands, and 9-12 days after feeding the larva was found in the circular muscular layer of the intestinal wall.
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  • M. KAGEYAMA
    1924 Volume 1 Pages 226-232
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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    Seit einigen Jahren hat eine Infektionskrankheit der Taube sogenannte Muguet, weit verbreitet und viele Tauben, insbesondere die von japanischem Militar Taube-untersuchungskommite, sind gestolben. Der Erreger dieser Infektionskrankheit wurde bei den europäisehen Autoren schon klar festgestellt und als die Ursache wurde Oidium Albicans vorschlagen.
    Die Symptome dieser in Japan vcrbreiteten Infektioyskrankheit sind viel kompliziert als das echte Muguet, und der Verlauf immer bosartig. In Japan, hat niemand bis hente als der Erreger dieser Krankheit Oidium albicans festgestellt. Ich habe unter die Leitung von Herrn Dr. Tagawa gearbeitet um den Erreger dieser Infektionskrankheit festzustellen. Ich habe zwei Arten Oidien und ein Stabchen aus den Muguet erkrankten erhalteten Material immer rein kultiviert und durch Tier-versuch habe ich versucht, entweder diese Keime für sich allein oder mit zusammen gegen Taube pathogen sind.
    Nach mannigfacher Versuche, habe ich beobachtet, dass beide Arten des Oidiums für sich allein gegen Taube schwache Pathogenitat bewirken. Wenn auch das Stäbchen für sich allein oder mit Oidium zur Taube injiziert ist, ruft die typische pathologische Veranderungen hervor. So scheint es mir dass diese zwei Arten des Oidiums als der Erreger dieser Infektinnskrankheit zweite Rolle spielen.
    Die Oidien, die ich isoliert habe, sind erste-und zweite typus nach der Klassifikation von Riketts entspricht.
    1. Erste Typus; Auf Sabouraudschem Nãhrboden wãchst diere Gruppe grauweisse trockene Belage, diese Pilze wachsen in den gewöhnliche Nahrböden, sowie auch besonders gut in Zuckernährboden und Milch, keine Gerinnung von letzteren hervorzurufen. Diese Gruppe hat ein geringe Vermögen, Zucker zu vergären. Im Lackmusmolke verandert es nicht die Farbe.
    2. Zweite Typus; Diese Gruppe wächst etwas schneller als die erste, in Form glänzendefeuchte weisse Belage, auf festem Nahrboden. In flussiger Nahrboden wächst diese Gruppe in der Form feinliche, niederschlagende Bodensatz oder diffus trÜbende Flocken. Im Lackmusmolke entwickelt es gut, schwache Rotung darzutun. Diese Pilze haben nicht Vermögen, Zucker zu spalten.
    Immer mit Oidien begleitend befindet es sich dieses Stäbchen im Lokal der erkrankten Taube. Diese Baktirien farben sich gut mit gebräuchlichen Anilinfarb-stoffen, jedoch nicht nach Gram, und haben Tendenz, die beiden Enden stark tingierend. Diese Bakterien haben Beweglichkeit. Durch Bildung von Gasen, die besonders gut bei Zusatz von Traubenzucker wachsen diese Bakterien. Bouillon wird getrubt, Milch keine Gerinnung verursacht. Lackmusmolke wird die Farbe verändert.
    Allgemeine Eigenschaften diesen Bakterien werde ich spater mit Herrn Hauptmann Shiba vorschlagen.
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  • T. Konno.
    1924 Volume 1 Pages 239-253
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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    In 1922 the author isolated a kind of bacteria from the internal organs of the domestic fowl that had died of a disease similar to the fowl chorela, and published the results of experimental study on this bacteria under the title of "Newly discovered fowl plague in Japan (Plague similar to "Huhner Typhus") " in the journal of the central veterinary association in Japan.
    Since then the same disease has been found in various parts of Korea. From the accurate observation the author came to the conclusion that this fowl plague in Korea may be the same as that found in Europe and America.
    The results of his observation and experimental study on this disease may be summarized as follows:-
    1. Since 1922 the occurence of the fowl typhoid has often been reported, and the author has found that the disease is widely spread in Korea., extending from the southern parts (Saishu island) to the northern end.
    2. The mode of propagation, the symptoms, the post-mortem and the bacteriological observation of this disease correspond almost entirely to those of the fowl typhoid prevailing in Europe and America.
    3. With regard to the difference between the fowl typhoid and the fowl cholera the writer stated his opinion in his previous paper that it is easy to distingish clinically the two diseases from the colour of dropping. But the precise observation shows that the bacteriological examinaton is most reliable to distinguish the difference between these diseases.
    4. From the experiment it is shown that the bone marrow of tibia is found to be the best material for the bacteriological examination.
    5. In order to stamp out this disease it is necessary to extirpate the carriers of bacteria which are easily detected by means of agglutination test which may be the most valuable method for this purpose.
    6. From the experiment it can be shown that the immuue serum and the vaccine are avairable for the prevention of this disease. Moreover the immune serum has obviously a curative power for this disease if used in an early stage or in a slight attack of the disease.
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  • YOSHIHIKO MATSUYAMA
    1924 Volume 1 Pages 253-265
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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    Although various works have been carried out by many authors dealig withthe composition of hen's egg, yet many points are left to be cleared up.
    This work was undertaken with the hope to get clearer conception of the metabolisia of hen's egg during the period of incubation.
    Three eggs were taken every three days from the incubator and the percentage composition of the chemical constituents such as water, ash, crude fat, cholesterol, lecithin, several groups of nitrogen in the protein and some kinds of amino acids are determined.
    These results are shown in the following table, (and are also expressed graphically in the original paper).
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  • K. NAITO, T. SHIMAMURA, K. KUWABARA
    1924 Volume 1 Pages 275-333,1
    Published: September 10, 1924
    Released on J-STAGE: March 10, 2008
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    The objects of the present experiment is to see the effects of polished rice feeding and the vitamin B-defect in the ration in horses, especially their bearings on the pathogenesis of experimental beri-beri and podophylitis.
    Five Corean pony mares were fed on the ration consisting of polished rice grains and rice straw well extracted with boiling water. The ration was supplemented with McCollum's salt mixture No. 185 and with vitamins A and C in form of cod liver oil and juice of natsumikan orange. The animals were at last affected, in the course of 4-12 months, with the so called polished rice disease. Clinical studies and analyses of blood and urine were carried out throughout the experiments, the chief results of which are summarized as follows:
    1) Clinical observations.
    The stage of incubation ranges from 4-12 months. The loss of apetite begins at from the 4th to the 100th experimental day and proceeds progressively. The body weight begins to decrease from the beginning of the experiment very slowly but steadily during the first two months, and later on promptly until the animals lose on an average 23% of their body weights and become affected with the disease. When the animals succumb the loss of the body weight attains to 30% of the initial.
    The body temperature has contrary to the often reported cases of avian beriberi, no tendency to decline throughout the incubation stage, and when diseased it becomes a little higher than the normal. The disturbances in the circulatory system is characteristic. After the evolution of the disease the pulse rate increases enormously and attains generally to 100-180. The pulse is irregular and intermittend. The second cardiac sound is rather high, while the dilatation can not be recognized.
    No Change in digestive function, except the loss of apetite, could be detcted. And diarrhoea, which is one of the characteristic disturbances in the B avitaminosis in the small experimental animals, has never occurred. Respiratory system is also not affected.
    The motor disturbance such as paralysis and paresis of hind legs is the second characteristic symptom of this disease. Horses may, indeed, be hungered until to death without causing paralysis of locomotor organs.
    The course of the disease is rather accute and ranges from one to twenty two days.
    2) The results of analyses of blood and urine.
    Change in blood sugar content is, in general, not detectable, though in some animals doubtful hypoglucemia in the early stage of the incubation, and hyperglucemia in very slight degree towards the outbreak of the disease were observed. Sugar torelance was also unchanged.
    Carbon dioxide content and oxygen capacity of the blood remained constant during the whole course of the experiment. Among nitrogenous matters non protein nitrogen, urea, creatinine and creatine in blood ; and total nitrogen, ammonia, urea, creatinine and creatine in urine were analyzed. Very slight variation in creatine metabolism was recognized. No special disturbance in the metabolism of inorganic matters in blood and urine, as phosphor, calcium, magnesium and chlorine, was detected.
    3) The treatment.
    The polished rice disease in the horse can be readily cured by the treatment with B vitamin preparate, such as Oryzanin of Dr. Suzuki. The cardiac disturbances and apetite are immediately recovered after the administration of the vitamin. The motor disturbances, however, are gradually restored, and the body weight shows, when a large quantity of Oryzanin is given, tendency to increase even on the same ration.
    4) Effects of hunger.
    To see effects of hunger two ponies were subjected to hunger. The animals lost 34% of the initial body weight during the course of 3 weeks, none of the morbid symptoms appeared. Emancipation only can, therefore, not be cause of motor disturbances. Metabolism seen from analysis of blood and urine is quite different in creatine and non determined nitrogen.
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