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Volume 36, Issue 6
Displaying 1-10 of 10 articles from this issue
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Yoshio KAISHIO1965 Volume 36 Issue 6 Pages 185-190
Published: June 25, 1965
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSDownload PDF (581K) -
Kazue Aso1965 Volume 36 Issue 6 Pages 192-197
Published: June 25, 1965
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSIt was the purpose of the present investigation to develop an in vitro procedure which could accurately estimate the nutritional value of Soybean meals. The experiments described in this report were designed to estimate the correlation between the water soluble nitrogen of soybean meals processed in ifferent conditions and the nutritive values of these meals when fed to chicks.
The increase in the growth rate of chicks and the reduction in water soluble nitrogen of the meals were good measures of the nutritive value of the meals as indicated by the significant negative correlation (-0.904 and -0.925 ; significance level 0. 01). Chick growth was maximum in this experiment when the water soluble nitrogen of the meals were between 1.3 and 1. 0 percent of the meal, but no differences were observed within the rView full abstractDownload PDF (514K) -
Methionine Supplementation and Digestibility of the Meals Kazue AsoKazue Aso1965 Volume 36 Issue 6 Pages 198-202
Published: June 25, 1965
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThe addition of methionine to a corn-underheated soybean meal type of diet for chicks did not overcome the growth depressing property of the underheated meals and a hypertrophy of the pancreas compared with chicks receiving heated meals. This result suggested that the action of the enzyme inhibitor was not necessarily associated with poor utilization of methionine in the underheated meals.
Using cocks with surgically prepared artificial anus, the digestibility of the meals was determined.
The digestibilities of the heated meals, particularly protein, were better than that of underheated ones.
These results suggested that contrary to some early reports the effect of inhibitors was a general effect on the liberation of all amino acids and not particularly on methionine.
Microscopic observations obtained on the pancreases of chicks fed raw soybean meal and heated soybean meal diet were shown. Pancreas of chicks fed raw soybean meal showed the depletion of zymogen granules.View full abstractDownload PDF (343K) -
Tateki CHIKAMUNE1965 Volume 36 Issue 6 Pages 203-209
Published: June 25, 1965
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThe effects of nursing environmental conditions on variability of body weight at 15 days of age and on the time of optical opening were studied using inbred, random bred and F1 hybrid between inbred lines of mice. The results obtaind are as follows :
1) The results of analysis of variance showed that genetical and postnatal maternal influence played important roles in determing both body weights at 15 days and at the time of optical opening. The difference between sex and interactions between the aboves were not significant.
2) A comparison of the coefficients of variation indicated that the more unfavourable the nursing environmental conditions became, the larger the ariation of body weight at 15 days of age in all groups, particularly the increase in variation being greater in the inbred groups.
3) Variation at the time of optical opening in the random and F1 hybrid populations seemed to increase in unfavourable nursing conditions. While, in the inbred, larger variations were always shown both in the favourable and unfavourable nursing environmental conditions.
4) When the more variable the nursing environmental conditions became, the greater the variation of body weight at 15 days of age increased in F1 and andom populations than in the inbred. It was suggested that heterozygous individuals had a capacity to develop their body size more flexibly to the given local conditions of nursing environment.View full abstractDownload PDF (603K) -
Yoshitsune HIROSE, Shozo SUZUKI, Eiichi UEYAMA1965 Volume 36 Issue 6 Pages 210-216
Published: June 25, 1965
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSA series of experiments was conducted to determine the validity of ratio technique, by using the chromogen and the chromium oxide as indicators, for estimation of forage intake of grazing animals,
Trial 1: The recovery of the chromogen in feces was determined by using two steers fed red clover as the experimental ration.
Nearly 100% of recovery was obtained.
Trial 2: The estimation of forage intake of tethering goat and sheep was made by the following three methods:
A. Green forage intake (g) = Estimated amount of forage in tethered grazing area before tethering by small plots clipping (g) -Forage residue after thered grazing (g).
B. Green forage intake (g) =Wet feces voided (g)×Chromogen units per gram of wet feces/Chromogen units per gram of green forage.
C. Green forage intake (g)=Cr2O3 administered(g)/Cr2O3(g)per gram of wet feces×Chromogen units per gram of wet feces/Chromogen units per gram of green orage.
The better results were obtained by the method B and C than the method A. The considerable daily variation of the intake of forage was noted by the method A.
Trial 3: The excretion pattern of Cr2O3 in feces was examined by using two lactating cows. Fecal grab samples were collected from rectum every three hours and Cr2O3 content was determined. Chromium oxide was administered once a day (at 3: 00 pm, 10g) or twice a day (at 6: 00 am and 3: 00 pm, 10g each). The hourly variation of Cr2O3 content in dry feces was less when administered twice a day than did once a day. There was no definite trend during 24 hours on the changes of Cr2O3 concentration in dry feces collected every three hours.
Trial 4: The determination of forage intake by a lactating cow was conducted by the simultaneous use of the chromogen and Cr2O3 as indicators. Two ctating cows were grazed on the orchard grass pasture. Chromium oxide was administered once a day (at 3: 00 pm, 10g) and fecal grab samples were llected twice a day (at 9: 00 am and 4: 00 pm). The method C in Trial 2 was applied to determine the forage intake. The better results were obtained as compared to conventional methods.View full abstractDownload PDF (485K) -
1. Effects of fundamental factors on reaction of chromium salt with DNP-amino acidYukichi SAKIMOTO, Tsugio OSUGI1965 Volume 36 Issue 6 Pages 217-221
Published: June 25, 1965
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThe reaction of Cr salt with DNP-amino acid was studied under various conditions. Sulfur dioxide-reduced liquors containing DNP-glycine were used. The amount of DNP-glycine combined with Cr, which was not removed with ether, was determined from the difference in the amounts of uncombined DNP-glycine. The amount of uncombined DNP-glycine was calculated from the difference in absorbancies which had been measured at a wave length of 350 mμ before an after extracton with ether. Then the effects of concentration of Cr, time of reaction, pH and addition of some organic salts upon the amount of reaction of Cr with α-carboxyl group were studied in comparison with relative values obtained for the combined DNP-glycine. The results obtained are summerized as follows:
1. The amount of combined DNP-glycine increased as the concentration of Cr became higher.
2. The reaction between them proceeded fast until the standing of the solution lasted for 72 hours and then it slowed down later.
3. The amount of combined DNP-glycine increased with rising of pH to a maximum value at nearly original pH 4 and then decreased.
4. The amount of combined DNP-glycine decreased by addition of Na-acetate and -oxalate.View full abstractDownload PDF (343K) -
2. Difference of reactivities of several kinds of DNP-amino acids with chromium saltYukichi SAKIMOTO, Tsugio OSUGI, Kenshi SHIOTANI1965 Volume 36 Issue 6 Pages 222-224
Published: June 25, 1965
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThis work was undertaken to determine the difference among reactivities of different kinds of DNP-amino acids with Cr salt. The solutions were prepared by adding each solution of several kinds of DNP-amino acids to S02-reduced liquor respectively. After allowing to stand, the amounts of DNP-amino acids combined with Cr were determined. The determination of the amount of combined DNP-amino acid, which was not extracted with ether, was made by the difference in the amounts of uncombined DNP-amino acids varing with time of reaction. The amount of uncombined DNP-amino acid was calculated from the difference in absorbancies which had been measured at a wave length of 350mμbefore and after extraction with ether.
The difference among their reactivities was evaluated by comparison with their combined amounts. The results obtained are as follows :
1. The amino acid with more carbon atoms per molecule did not combine easily with Cramong several kinds of the DNP-amino acids.
2. The amount of DNP-serine combined with Cr was greater, because of presence of OH group within that molecule, than the amount of DNP-alanine.
3. Monoamino dicarboxylic acid combined with Cr much more than monoamino mono-carboxylic acid at a range of higher pH in the acidic solution.View full abstractDownload PDF (206K) -
II. Preparation of Anti-Q Agglutinin by injecting Animals with Q-AntigenShozo SUZUKI, Kazue TANAKA, Seiki WATANABE, Tsutomu OIKAWA, Soichi WAT ...1965 Volume 36 Issue 6 Pages 225-232
Published: June 25, 1965
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSIt is very difficult to obtain anti-Q agglutinin from the normal sera of pigs. Present experi-ment on preparation of anti-Q agglutinin by injecting animals with human Q red cells was carried out. The following results were obtained.
1). Anti-Q agglutinin was produced in immunosera of 7 rabbits oui of 10 by injecting with human ONQ red cells and 3 pigs out of 4 with human OMNQ red cells. In normal sera of these 3 pigs, anti-Q agglutinin of low titer existed.
2). Anti-Q agglutinin with quite weak reaction was prepared in immunosera of 5 rabbits out of 8 injected with stroma of human OMNQ red cells.
3). Twenty eight rabbits, 2 goats and 10 guinea pigs in which anti-Q agglutinin was not recognized in their normal sera were injected with human OMNQ red cells, but anti-Q agglutinin could not be prepared in their sera.
4). To prepare anti-Q agglutinin of high titer by injecting animals with Q antigens, it seemed to be necessary that anti-Q agglutinin being contained in their normal sera.View full abstractDownload PDF (517K) -
I. Gene Constitution of the Island Type Native HorsesKen NOZAWA, Kozaburo ESAKI, Noboru WAKASUGI, Shigeyuki HAYASHIDA1965 Volume 36 Issue 6 Pages 234-242
Published: June 25, 1965
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSIn the small islands south and west of Kyushu, Japan, the native horses of pony type have been raised by farmers. These horses are considered to epresent the oldest type of native horses in Japan. So, in order to investigate the peculiarities in gene constitution of that horse breed, the authors made expedition to Tsushima Islands, Tane and Kuchi-erabu Islands, Tokara, Amami and Ryukyu Archipelagoes, and made field surveys on the cost colors and blood types of both pure native and hybrid horses kept by inhabitants of these islands. And, in addition, the authors consulted for comparison the color and blood-typing records on various horse breeds so far published by several antecedents. The results of the investigation are as follows :
1. The populations of the native horses have not recessive black gene (a) nor dominant dilution gene (D), but as a result of hybridization with mproved breed introduced from Japanese main inlands the genes a and D have flowed into the populations.
2. As the populations of the native horses they are composed only of bay and chestnut horses, they are polymorphic with regard to the B-b locus, the frequency of appearance of chestnut color in these populations being much higher compared with that in the improved horse breeds in Japan.
3. The frequency of horses with the erythrocyte antigen Ul in the native breed is nearly zero, whereas in improved breeds it is over 90 percent. Therefore, the population resulted from hybridization between native breed and improved one has individuals with that antigen in an intermediate frequency.View full abstractDownload PDF (742K) -
Ichiro GOTO1965 Volume 36 Issue 6 Pages 243-244
Published: June 25, 1965
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESS
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