1) The concentrations of nitrate in grasses, legumes, soiling crops and silage were determined to get the approximate informations on the concentrations of nitrate in roughage practically used in Japan.
2) Samples of various grasses and legumes were obtained from the fields of twenty-eight Agricultural Experiment Stations. These stations were located at Hokkaido, Akita, Iwate, Niigata, Ibaragi, Tochigi, Chiba, Gifu, Mie, Shiga, Nara, Kyoto, Osaka, Wakayama, Hyogo, Shimane, Hiroshima, Tottori, Kagawa, Kochi and Kumamoto prefectures. The samples-of soiling crops and silage were collected from the fields of two experimental farms of Kyoto University and from the fields on the outskirts of them. The number of the samples was one hundred and sixty-four for grasses and legumes and seventy-seven for soiling crops and silage.
3) The concentrations of nitrate in grasses ranged from 0.02 to 4.11 per cent as potassium nitrate in dry matter and those in legumes ranged from 0.18 to 3.60 per cent. The average concentrations of nitrate incultivated grasses and legumes, excluding some exceptional concentrations, were 0.36±0.27 per cent for grasses and 0.61±0.27 per cent for legumes. The concentrations of nitrate in grasses seemed to be less than those in legumes. There were several samples containing nitrate exceptionally high and thoses samples were apparently more in grasses, and the leading species of them were Italian ryegrass in grasses and Ladino clover in legumes.
4) Except some exceptional concentrations, the concentrations of nitrate in Italian ryegrass were 0.33±0.21 per cent as potassium nitrate in dry matter. Those in orchard grass and Kentucky 31 fescue were 0.37±0.29 and 0.45±0.26 per cent. Those in Ladino clover seemed to be high and were 0.77±0.30 per cent except some exceptional ones. Those in red clover were 0.51±0.21 per cent with no exception.
5) According to the results of this research, the concentrations of nitrate in plants in the southwestern part (Chugoku, Shikoku and Kyushu districts) seemed to be fairly higher than those in the northeastern part (Hokkaido, Tohoku, Kanto, Chubu and Kinki districts). It was also remarked that there were some experiment stations where exceptionally-high-nitrate-containing plants were produced. These stations were located at Hiroshima, Kagawa and Kumamoto prefectures.
6) The concentrations of nitrate in soiling crops were considerably higher than those in grasses and legumes. Those in soiling oats generally used for feeding ranged from 0.27 to 2.52 per cent, averaging 1.29±0.56 per cent. Those in soiling corn ranged from 0.60 to 1.41 per cent, averaging 0.95 per cent. In this experiment, soiling soybean seemed to contain less nitrate. The concentrations of nitrate in turnip were variable and it seemed that turnip was one of the most nitrate-accumulating plants. Those in silage were considerably low and were less than 0.60 per cent.
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