Nihon Chikusan Gakkaiho
Online ISSN : 1880-8255
Print ISSN : 1346-907X
ISSN-L : 1880-8255
Volume 40, Issue 6
Displaying 1-6 of 6 articles from this issue
  • Tokuzo HASHIZUME
    1969 Volume 40 Issue 6 Pages 221-228
    Published: June 25, 1969
    Released on J-STAGE: March 10, 2008
    JOURNAL FREE ACCESS
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  • Yasuyuki SASAKI
    1969 Volume 40 Issue 6 Pages 229-237
    Published: June 25, 1969
    Released on J-STAGE: March 10, 2008
    JOURNAL FREE ACCESS
    Twenty Holstein calves were fed on limited amount of whole milk, green forage or orchard grass hay and grain. They were sacrificed at 1, 4, 8, 13 and 23 weeks of age to determine the development of the uptake of VFA by the liver slices and the rumen epithelium. Five hundred mg of the liver slices or 2g of the rumen epitheliun were incubated at 38°C for 3hrs in Krebs-Ringer-phosphate buffered at pH 7.2 in an atmosphere of oxygen. Two hundred μ-moles of acetate, propionate, butyrate, or an equimolar mixture of these three acids were added to medium.
    1. In the liver slices, the utilization of acetate most increased with age, compared with that of propionate and butyrate. The utilization of acetate by the liver slices from the calves at 1 or 4 weeks of age was lower than at 8 weeks or more weeks of age, and was markedly increased from 4 to 8 weeks of age. Propionate and butyrate were utilized by the liver slices, but the utilization of them showed no marked change due to age.
    2. In the rumen epithelium, the butyrate utilization most increased due to age. The utilization of butyrate in the newborn calves was extremely low, and increased until it reached adult level by 8 weeks of age. The utilization of acetate and propionate did not increase with the increase of age.
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  • Suezo KOSAKA, Hiroshi KANAGAWA, Tsune ISHIKAWA, Tatsuo HOSODA
    1969 Volume 40 Issue 6 Pages 238-242
    Published: June 25, 1969
    Released on J-STAGE: March 10, 2008
    JOURNAL FREE ACCESS
    A bull born co-twin to a freemartin was noted to display pattern of blood type which was different from his genotype. The leucocyte cultures of this bull exhibited mitotic cells exclusively of female origin (XX type). However, a study of the sex ratio of the offspring of this male during the past five years showed no preponderance of female progeny, suggesting that the interchange of cells resulting in an apparent displacement of the blood cell precursors in the male twin does not extend to the germ line.
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  • Akira AKASHI
    1969 Volume 40 Issue 6 Pages 243-248
    Published: June 25, 1969
    Released on J-STAGE: March 10, 2008
    JOURNAL FREE ACCESS
    The effect of lysozyme in the presence or absence of NaCl and NaNO2 as a preservative agent for cooked sausage was studied. The addition of lysozyme at levels of 50 and 200ppm in combination with 3% NaCl and 12.5ppm NaNO2 plus heat was more effective in preserving sausage for four weeks at 10°C than additions of either lysozyme or a mixture of NaCl and NaNO2 plus heat. Omitting the effect of heat, lysozyme retarded microbial growth to greater extent than did the standard preservative mixture of NaCl and NaNO2.
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  • V. Increase in non-protein nitrogen and the deteriorating effect on the quality of silage due to protein addition at ensiling
    Yoshinobu OHYAMA, Shigehiko MASAKI
    1969 Volume 40 Issue 6 Pages 249-254
    Published: June 25, 1969
    Released on J-STAGE: March 10, 2008
    JOURNAL FREE ACCESS
    Changes in nitrogen distribution in materials during ensilage were investigated in eight laboratory ensilage experiments where the effects of 0, 2 and 5% gluten addition at ensiling were compared.
    The ratio of volatile basic nitrogen to total nitrogen in silage was inversely related to lactic acid content, and high value of the ratio was also accompanied by the presence of butyric acid (and higher VFA's).
    The extent of breakdown of the added gluten was, in general, much greater than that of the protein in the grass, suppose that the extent of breakdown of the grass protein is the same regardless of the gluten addition. However, it was not necessarily accompanied by the deteriorating effect on the silage quality by the gluten addition, although less breakdown corresponded with the good quality.
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  • Kiyoshi TAKESHITA, Yasuyuki SASAKI
    1969 Volume 40 Issue 6 Pages 255-258
    Published: June 25, 1969
    Released on J-STAGE: March 10, 2008
    JOURNAL FREE ACCESS
    Present experiment was conducted to make clear the quantitative changes in blood fructose and glucose of the newborn calves. Six Holstein calves were used and blood samples were taken immediately after birth and also 10, 20, 30 and 50 hours after birth. The amounts of fructose and glucose were determined by means of the resorcin-hydrochloric acid method and the glucose oxidase method, respectively.
    A large amount of fructose (53.8±16.5mg/100ml in whole blood: 54.5±19.3mg/100ml in plasma; mean±S.D.) was observed immediately after birth. The amount of fructose in blood decreased very rapidly and almost completely disappeared in 10 hours after birth.
    The amount of glucose was 57.8±9.9mg/100ml in whole blood and 63.0±14.6mg/100ml in plasma immediately after birth and increased rapidly. The maximum amount (112.8±25.6mg/100ml in whole blood: 120.3±24.9mg/100ml in plasma) was observed at 30 hours after birth.
    Based upon the above findings and the results of the previous studies, it is probable that the rapid disapperance of blood fructose in newborn calves is due mainly to the urinary excretion and this disappearance seems to cause the increase of blood glucose.
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