Five samples of two varieties of italian ryegrass (late and early cut, A and B), timothy, rice straw and alfalfa hay cube and cell wall materials isolated from these samples by neutral detergent solution were used. 0.5g samples were placed in 500 ml tall beaker and 100ml of 5% Na2SO3 solution or water were added and boiled on heater for 30 or 60 minutes (First Step). The residue was suspended with 0.2% cellulase solution in 50ml polyethylene tube placed in a shaking apparatus at 40°C for 2 to 8 hours (Second Step). We analysed the influence of pretreatment of first step in connection with
in vivo dry matter digestibility (DMD) and kinetics of second reaction. From the results of these experiments, we considered as follows:
1. Thirty minutes were enough as boiling time in the pretreatment with both water and 5% Na2SO3.
2. In the case of hot water pretreatment, Two Step DMD (First Step DMD%+Second Step DMD%) were never as much as in vivo DMD% and between the two, the following correlation was observed at 6-hr cellulase hydrolysis, r=0.959 P<0.05.
3. In the case of 5% Na2SO3 pretreatment, the measured DMD% of three samples such as rice straw, timothy and Italian ryegrass A almost agreed with
in vivo DMD% for 2 to 6-hr hydrolysis time and as another samples, by controlling cellulase concentration and/or hydrolysis time, it may be possible to estimate the in vivo DMD%.
4. In both treatment, cellulase hydrolysis was expressed as x=ctn (x: rate of digestion %, t: hydrolysis time hr., c, n: constant). In regard to the values of c and n, 5% Na2SO3 pretreatment were higher than hot water pretreatment.
5. After pretreatment with 5% Na2SO3, cellulase hydrolysis could be expressed by first order reaction as follow: C=Coe-kt (C: residual reactive DM%, Co: whole reactive DM %, R: rate constant, t: hydrolysis time hr).
6. By 5% Na2SO3 pretreatment, lignin and silica were dissolved out partially in the case of grasses, as a result, increase in rate of reaction was observed in the following cellulase hydrolysis. But in the case of alfalfa hay cube, there was not observed such a phenomenon by pretreatment.
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