The objective of the present study was to investigate the effect of dietary long chain fatty acids on fatty acid composition of liver and plasma lipids in birds. Single comb White Leghorn male chicks were fed a fat-free diet and diets containing stearic, lionoleic or margaric acid, and liver lipid components and liver and plasma fatty acid distributions were compared. The fractionation of total lipids and analysis of fatty acid distribution were performed by thin layer chromatography and gas liquid chromatography, respectively. The fatty acid feeding did not affect the body weight gain, liver size and liver lipid components. Wet liver contained 5.9-7.8% total lipids, which contained 41-50%, 27-30%, 9-12% and 1-2% of phospholipids, trigricerides, free cholesterol and cholesterol esters, respectively. When margaric acid (17:0) was fed, 17:0 and heptadecenoic acid (17:1) appeared in every lipid fractions of liver and plasma, and distribution values of these acids were not significantly different between the lipid fractions of liver. In blood plasma of the 17:0 fed chicks, however, significantly higher distribution values of 17:0 and of 17:1 were observed in the trigryceride fraction and in the cholesterol ester fraction, respectively. Dietary stearic acid (18:0) did not show any effect on the distribution of 18:0 in every lipid fractions of liver, but showed a significantly higher distribution value of 18:0 in the free fatty acid fraction of plasma. When linoleic acid (18:2) was fed, every lipid fractions of liver and plasma contained 18:2; especially a significantly higher distribution value was observed in the phospholipid fraction of liver. Dietary margaric and linoleic acids tended to decrease the distribution value of endogenously synthesized 16:1 and 18:1 in liver. The phospholipid fraction of plasma contained a substance which was considered as eicosatrienoic acid (20:3), and the distribution value of this substance was significantly lower when the linoleic acid diet was fed than when the other diets were fed.
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