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Volume 49, Issue 2
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Keiichi TANAKA1978 Volume 49 Issue 2 Pages 81-88
Published: February 25, 1978
Released on J-STAGE: March 10, 2008
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Yukio TSUNODA, Akira IRITANI, Yoshimasa NISHIKAWA1978 Volume 49 Issue 2 Pages 89-95
Published: February 25, 1978
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThe present study was carried out to estiblish an effective method for the production of large number of fertilized eggs in the rabbit. Three hundred and ten mature Japanese White rabbits (2.5-4.0kg) were used to induce superovulation following the procedured indicated in Fig. 1. The results obtained are as follows. 1. In rabbits treated with FSH and LH or HCG, more than 30 fertilized eggs were obtained in one animal. LH could be replaced by HCG without decrease in the number of eggs ovulated and fertilized. 2. Decrease in the number of eggs ovulated in the case of repeated superovulation was prevented to certain extent by combined adminstration of estrogen at the time of first mating or recovery of eggs. 3. Two, 4-, 8-, 16-celled eggs, morula and blastocyst were respectively obtained 21, 24. 5, 41, 46, 48 and 69 hrs after mating. Fertilized eggs were recovered 67 hrs after mating from uterus, but unfertilized eggs were recovered even 46 hrs after mating. 4. Rabbit fertilized eggs recovered from superovulated dose developed into fetuses and live-born when they were transferred into recipient rabbits.View full abstractDownload PDF (453K) -
Yukio TSUNODA, Akira IRITANI, Yoshimasa NISHIKAWA1978 Volume 49 Issue 2 Pages 96-103
Published: February 25, 1978
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSPresent experiments were performed to examine the possibility of implantation of rat eggs transferred into rabbit uterus and examine the possibility that the limited development of rat eggs transferred into rabbit uterus was due to an immunological rejections. Results obtained are as follows. 1. Rat eggs transferred into one oviduct of recipient pregnant rabbits following the transfer of rabbit eggs into another uterus could not induce uterine swelling on the side of uterine horn into which rat eggs were transferred. 2. While there could not be detected any precipitation bands against rat ovary extract after immunoelectrophoresis in the rabbit serum collected before the transfer of rat eggs, rabbit serum collected after the 2nd transfer of rat eggs produced 1-3 precipitation bands, and the same patterns of the precipitation bands were detected against rat ovary, liver, kindney and fetus. 3. There was no appreciable difference in the effect of rabbit serum on the development of rat fertilized eggs in vitro. However, the development of rat blastocysts into fetuses preincubated with rabbit serum collected after the 2nd transfer of rat eggs was clearly inhibited when they were retransferred into rat uterus. 4. The rates of implantation and parturition of rabbit eggs were inferior compared to the normally pregnant rabbits by egg transplantation when rabbit and rat fertilized eggs were transferred respectively into each genital tract of the rabbit. This trend was remarkable when the same transfer was repeated. However, the lower rates might to due to rather physiological disturbances resulted from repeated surgery than possible accumulation of mmunological rejections. It was suggested from the above results that a apecies-specific antibody appeared after transfer of rat eggs into rabbit uterus especially when the transfer was repeated, and that the limited development of rat eggs transferred into rabbit uterus was partially due to an immunological rejection.View full abstractDownload PDF (555K) -
Hideo YANO, Shoyo SAKURAI, Ryoji KAWASHIMA1978 Volume 49 Issue 2 Pages 104-109
Published: February 25, 1978
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThe effects of sodium bicarbonate administration on blood acid-base balances and serum mineral concentrations were examined using three sheep in trial 1. In trial 2, six sheep were used, and the effects of sodium bicarbonate on urine and serum mineral concentrations were studied in sheep given low and high calcium rations. As dietary sodium bicarbonate increased, blood pH and H2CO3 concentrations tended to become higher and blood HCO3-concentrations and urine pH elevated significantly (P<0.05). Serum calcium concentrations had a trend to decrease and, adversely, serum phosphorus tended to increase as sodium bicarbonate was given. The administration of sodium bicarbonate had the effect of diminishing urine calcium concentrations and excretion. Urine phosphorus concentrations and excretion tended to increase and urine magnesium decreased by the administration of an alkalizing agent. It may be concluded that the decreases in urine calcium and magnesium were induced by an alkalotic condition of animal body, but urine phosphorus concentrations and excretion were not lowered by the feeding of an alkalizing agent. Therefore, sodium bicarbonate may have little effect in reducing the occurrence of urolithiasis.View full abstractDownload PDF (325K) -
Hisao ITABASHI, Makoto KANDATSU1978 Volume 49 Issue 2 Pages 110-118
Published: February 25, 1978
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSAn unknown ninhydrin positive peak on the chromatograms of amino acid analyzer of alkalified rumen fluid distillate of goats was isolated as DNP-derivative and identified as methylamine. Under normal feeding condition, its concentration in the rumen ranged 0.1-3.9 mgN/100ml of rumen fluid and the proportion of methylamine in total volatile base, or apparent ammonia, ranged 0.5-13% during post-feeding. When ammonium salt was administered into the rumen with hay-concentrate ration, these values were increased up to 8.1 mgN/100ml and 25.8% respectively. Concentrations of ammonia and methylamine when aspartic acid or alanine was administered into the rumen in place of concentrate mixture (control) were not markedly different from the the control. In the case of arginine, glutamic acid or glycine administration, these concentrations were depressed as compared to the control. There were no distinct differences in the concentration of methylamine between the faunated and unfaunated goats. 14C from 14C-chlorella protein hydrolyzates, U-14C-alanine, 2-14C-glycine or 14C-sodium bicarbonate was incorporated into methylamine in in vitro incubation with rumen micro-organisms. When the washed suspensions of rumen bacteria or protozoa were incubated with 14C-chlorella protein hydrolyzates, the radioactivity in methylamine appeared only in the case of bacteria suspensions. After the addition of 15N-ammonium citrate into the rumen, the incorporation of 15N into methylamine was observed during 1-9hr.View full abstractDownload PDF (479K)
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