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Volume 52, Issue 6
Displaying 1-14 of 14 articles from this issue
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Tatsuro SHIMAOKA, Kazuei TANAKA, Kenji TSUNODA, Michio OTAKE, Shozo SU ...1981 Volume 52 Issue 6 Pages 413-418
Published: June 25, 1981
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSBlood protein polymorphisms of nine systems (pre-albumin-I, pre-albumin-II, acidic esterase, transferrin, arylesterase, alkaline phosphatase, hemoglobinβ, X-protein and NADH diaphorase) for 273 sheep of six breeds which have kept in Japan were investigated by means of starch gel electrophoresis and their variations were compared among several breeds. The degree of homogeneity and genetic similarities among six sheep breeds were discussed by using the gene frequencies in nine blood protein systems. Results obtained are as follows: 1. The gene frequencies of AcEsF, ArES+, HbβF and DiaS allele in Finnish Landrace breed were higher than those of the other breeds, and the frequency of ApaseB allele in Cheviot breed was the highest of them all. In transferrin system, the frequencies of TfA allele in Romney Marsh breed, TfC allele in Border Leicester and Finnish Landrace breeds, and TfD allele in Corriedale and Cheviot breeds were the highest, respectively. In pre-albumin-I, pre-albumin-II and X-proteinsystems, the frequencies of those in their breeds developed a tendency to be the same. 2. The homogeneity index of Border Leicester breed was the highest (0.608) in six breeds, and Finnish Landrace breed was the lowest (0.206) of them all. 3. From the results of genetic similarities among six sheep breeds, it was made clear that Border Leicester, Romney Marsh, Cheviot, Corriedale and Suffolk breeds constituted one group which was entirely different from Finnish Landrace breed. Within their breeds, the group of Cheviot and Corriedale breeds was different from that of Romney Marsh and Border Leicester breeds, and closer to Suffolk breed.View full abstractDownload PDF (418K) -
Mineo HASHIGUCHI, Osamu KOGA, Hisayoshi NISHIYAMA1981 Volume 52 Issue 6 Pages 419-424
Published: June 25, 1981
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThe effect of exogenous testosterone propionate (TP) on the level of gonadotropic hormone (GTH) of the anterior pituitary was examined, using male quail in which gonadal development was stimulated by changing the photoperiod from 8L (5:00-13:00): 16D (8hr light and 16hr darkness) to 14L (5:00-19:00): 10D. In the quail maintained under 14L:10D for 2 weeks, an increase in the level of pituitary GTH was observed 3hrs after a single injection of 10μg of TP. When TP was injected at various times during the day, the injection performed at the onset of light (5:00) caused a significant increase in the level of GTH. When the onset off light was shifted from 5:00 to 17:00 (10D:14L), the level of GTH increased following the injection of TP conducted at the onset of light (17:00). From these results, it is concluded that the level of pituitary GTH of the male quail kept under a long-day photoperiod may be increased by the injection of a small amount of TP, and the degree of the increase may very during the day in relation to the onset of light.View full abstractDownload PDF (423K) -
Kei-ichi SHIMAZAKI, Kinjiro SUKEGAWA1981 Volume 52 Issue 6 Pages 425-430
Published: June 25, 1981
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSBinding experiments were performed in order to estimate the amount of bovine immunoglobulins (IgG1, IgG2 and IgM) bound to Staphylococcus aureus. Also the interaction of immunoglobulins with Staphylococcal Protein A was tested by the methods of slide agglutination test, tube precipitation test, and double diffusion and radial diffusion tests on agar gels. No reaction of IgG1 and IgG2 with Protein A was observed and it is concluded that bovine IgG1 and IgG2 do not from insoluble complexes with Protein A. On the other hand, binding experiments showed that IgG1, IgG2 and IgM interact with Staphylococcus aureus. Immunoglobulins from mastitic milk and colostrum showed no difference of binding capacity using the same cell system. The strength in binding capacity was IgM>IgG2>IgG1.View full abstractDownload PDF (970K) -
Seiki WATANABE, Hiroyasu SAEGUSA, Kou NAKAJIMA, Setsuo IWASAKI, Shuhei ...1981 Volume 52 Issue 6 Pages 431-437
Published: June 25, 1981
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThe concentrations of free amino acid, potassium and sodium in red blood cells of sheep were analyzed, and the relation between the potassium type of red blood cells and amino acid type was investigated. Blood samples were obtained from sheep raised at the Laboratory of Animal Physiology of Tokyo University of Agriculture and Happy Sheep Farm in Hokkaido. 1) In paper electrophoretograms, sheep red blood cells were classified into two types according to the pattern of free amino acid in red cells, the first type was called Lys- type possessed three zones of amino acid, and the second type was called Lys+ type possessed four zones of amino acid. Lys+ type red cells contained heigher concentration of free amino acid than Lystype red cells, and the concentration of amino acid of Lys+and Lys-type was 23.23±3.59 mM/l and 3.32±0.97mM/l, respectively. 2) According to the ratio of potassium and sodium concentration, the red cells of sheep were classified into two types; HK and LK types. The ratio of Na/K in HK type was less than 2, and that in LK type more than 2. 3) The relation between K types and amino acidd types of the red cells of sheep was examined. K, Na and K+Na concentration in the red cells of Lys+ type were always lower than Lys- type in the both of HK and LK type of red cells.View full abstractDownload PDF (998K) -
Masamitsu SATO, Junji MASAKI1981 Volume 52 Issue 6 Pages 438-446
Published: June 25, 1981
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSProperty of acid mucopolysaccharide of glycoprotein in bovine cervical mucus during estrous cycle was studided. The concentrations of protein, sugar, sialic acid, hexosamine and uronic acid in mucus decreased in estrus. By means of cellulose acetate electrophoresis of mucus, one glycoprotein was detected in α-globulin fraction. This is possibly an acid glycoprotein, since it gave a positive Alcian blue stain. By means of cellulose acetate electrophoresis of the hydrolysates of mucus one acid mucopolysaccharide was detected, in which the migration pattern was different from that of hyaluronic acid, chondroitinsulfuric acid and heparin. Galactose was detected in the hydrolysates of acid mucopolysaccharide by paper and gas chromatographies.View full abstractDownload PDF (1405K) -
Kunitada SATO, Masaru MIYAKE, Nobuo TUNODA, Akira NAKAGAWA, Toshiharu ...1981 Volume 52 Issue 6 Pages 447-450
Published: June 25, 1981
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSForty-three colts aged 25-28 months were used for the determination of testicular size, semen characteristics (semen volume, pH, sperm motility, sperm concentration, percentages of abnormal spermatozoa and those with cytoplasmic droplets), and levels of serum steroid hormones. Following the observation of semen characterstics, colts were classified into two groups; Group 1 (G1) with good sperm motility and Group 2 (G2) with inferior sperm motility. The results are as follows: 1. There was no significant difference in the testicular size between right and left testes, and/or between two groups. 2. Significant differences were found in the motility of spermatozoa, and in the percentage of spermatozoa with tail abnormalities between two groups (G1 and G2). 3. Concentrations of serum testosterone and estrogens were measured in the above two groups of colts and in 5 stallions (Group 3: G3) which have been used for breeding. There were no significant differences in the concentrations of serum testosterone and estrogens among three groups (G1, G2 and G3). However, steroid levels of G2 were slightly lower than those of the remaining two groups, G1 and G3. It is suggested that the repeated measurements of the testicular size, the semen characteristics and the levels of steroid hormones may be usefull tools for the early selection of maturing stallion.View full abstractDownload PDF (251K) -
Effect of Pre-Slaughter Temperature Treatments on Postmortem Meat Quality of Quail Pectoralis MuscleToshiro SUZUKI, Ikuzo KAMOI, Shinnojo KIHARA, Tetsujiro OBARA1981 Volume 52 Issue 6 Pages 451-458
Published: June 25, 1981
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSOne hundred twenty (male: sixty, female: sixty) quails at eight weeks of age were equally randomized into the following faur treatments: 1) Control at 20°C; 2) Low temperature group at 4°C for 2 hours prior to slaughter; 3) High and Low temperature group which was first subjected to high temperature at 40°C for 1 hour and then to low temperature at 4°C for 1 hour; 4) High temperature group that was treated at 40°C for 1 hour prior to slaughter. The effect of pre-slaughter temperature treatments on postmortem meat quality was determined by measurement of pH, water holding capacity, water soluble protein extractability, transmission value, moisture, crude protein, ATP, glycogen, lactic acid, meat color, and total pigment. The high temperature group was noted to have a lower muscle pH, lower water holding capacity, higher transmission value and pale meat color than thes observed in other treatments. The meat quality of high temperature group showed the same deterioration as PSE meat. The low temperature group resulted in high muscle pH, high water holding capacity, low transmission value and good meat color. The quality of low temperature group was very different from that of the high temperature group. On the other hand, the quality of high and low temperature group was almost the same as that of the control and low temperature groups. These results indicate that the low temperature treatment prior to slaughter can restore the meat quality of the heat stressed quail.View full abstractDownload PDF (580K) -
Shu FURUYA, Seiya TAKAHASHI, Kenichi KAMEOKA1981 Volume 52 Issue 6 Pages 459-466
Published: June 25, 1981
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSDigestibility of crude protein (CP) and energy in various rations was compared for growing pigs weighing 20-30kg, chickens fitted with artificial anus weighing 1.2-1.5kg and rats weighing 180-250g. Also, the relationship between digestible energy (DE) and total digestible nutrients (TDN) was examined in the pig experiment. 1) The relative response in digestibility of the three species was indicated by the following correlation coefficients for CP and energy; pigs-chickens 0.98 and 0.99, pigs-rats 0.99 and 0.97 and chickens-rats 0.99 and 0.99, respectively. 2) The digestibility of CP by chickens was significantly (P<0.05) higher than that by rats and the digestibility of energy by chickens was significantly (P<0.05) lower than that by pigs and rats. The mean digestibility for 12 rations by pigs, chickens and rats was 81.4, 84.7 and 82.6% in CP, respectively and 88.4, 86.8 and 88.9% in energy, respectively. 3) The CP digestibility of rations containing high levels of lucern meal was considerably reduced in pigs. 4) The result of pig experiment using 16 rations indicated that DE (kcal/g DM) was closely related to digestible crude protein (DCP, % DM) and TDN-DCP (% DM) with a multiple correlation coefficient of 0.98: DE=0.0425(TDN-DCP)+0.0597DCP-0.1255View full abstractDownload PDF (538K) -
Hiroshi SATO, Shogo HANASAKA, Teruhisa IMAMURA1981 Volume 52 Issue 6 Pages 467-471
Published: June 25, 1981
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSIn Holstein calves, noradrenaline (NA) was injected subcutaneously at a dose of 0.1mg/kg at 1, 8, 18, 30 and 45 days of age, and the changes in rectal temperature, packed cell volume (PCV) and plasma free fatty acid (FFA), glucose and lactate levels were measured. NA-injection caused increases in all these measurements. An increase in rectal temperature was highest in 8-days old calves, and an elevation in PCV and FFA level was higher in older calves. At 1-day old, plasma lactate level was higher than thereafter, and NA-induced elevation in glucose level was sustained continuously. NA-induced elevation in plasma glucose level was larger in younger than older calves. There was no significant relationship between changes in rectal temperature and plasma metabolite levels after NA injection.View full abstractDownload PDF (311K) -
Kazuaki TAKAHASHI, Yukio AKIBA, Tatsuro MATSUMOTO1981 Volume 52 Issue 6 Pages 472-477
Published: June 25, 1981
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThree experiments were undertaken to investigate effects of dietary fat on the in vitro uptake rate of 14-palmitic acid into the lipid soluble fraction of the liver and the adipose tissue, and on lipoprotein lipase activity in growing chicks. Three kinds of diet, a low fat diet (containing soybean oil 5.5% of total ME intake), a lard diet (containing soybean oil and lard, 5.5% and 27.3% of total ME intake, respecitvely) and a coconut oil diet (containing soybean oil and coconut oil, 5.5% and 27.3% of total ME intake, respectively), were used in these experiments. The chicks were given one of these diets to be isocaloric and isonitrogenous for 14 days. Lipoprotein lipase activity in the adipose tissue was not changed by the amount and the type of dietary fat. The in vitro uptake rate of palmitic acid into the liver and the adipose tissue was not affected by feeding the low fat, the lard or the coconut oil diet. This rate, however, increased with the rise of palmitic acid concentration in the medium. These results suggested that the in vitro uptake of fatty acids into the lipid soluble fraction of liver and adipose tissue was influenced by the substrate concentration rather than the amount and the type of dietary fat.View full abstractDownload PDF (369K) -
Makoto MIZUTANI, Hidehiko UMEZAWA, Shigemi KURAMASU1981 Volume 52 Issue 6 Pages 478-479
Published: June 25, 1981
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSDownload PDF (134K) -
Tuguyoshi SUGANO, Kinichi WATANABE, Yasuhiro MINESAKI, Tokukazu IZUMI, ...1981 Volume 52 Issue 6 Pages 480-482
Published: June 25, 1981
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSDownload PDF (170K) -
Masayuki KITAGAWA, Ryoji KAWASHIMA1981 Volume 52 Issue 6 Pages 483-485
Published: June 25, 1981
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSDownload PDF (237K) -
1981 Volume 52 Issue 6 Pages 486
Published: 1981
Released on J-STAGE: April 21, 2008
JOURNAL FREE ACCESSDownload PDF (33K)
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