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Volume 53, Issue 7
Displaying 1-9 of 9 articles from this issue
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Yukio AKIBA1982 Volume 53 Issue 7 Pages 449-461
Published: July 25, 1982
Released on J-STAGE: March 10, 2008
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Hisashi HAMADA, Shinji MURAYAMA, Yoshiyuki SASAKI1982 Volume 53 Issue 7 Pages 462-473
Published: July 25, 1982
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThe adrenalectomized animal is of significance as an experimental animal in elucidating the role of glucocorticoids in regulatory mechanisms for glucose metabolism in ruminants. The methods of adrenalectomy, including the surgical procedure, postoperative management, longer prognosis and completeness of adrenalectomy tests, are discussed from our experiments on 30 cases of sheep adrenalectomized. The results are as follows. 1) Bilateral adrenalectomy was performed in double-stage operations, allowing 7 to 14 days for recovery between operations. 2) The surgical approach to the adrenal glands which was adopted was the posterior method. 3) Of the 30 wethers adrenalectomized, five died as a direct result of surgery. 4) In the postoperative management, procaine penicillin-G and sulfa medicine were administrated as chemotherapy for bacterial infections. After the removal of the second adrenal gland, deoxycorticosterone acetate (DOCA) and cortisol acetate were also used as the hormone replacement therapy in addition to the above therapy. 5) Body temperatures which increased after the operation returned to the normal level about one week later and the adrenalectomized animals had gained a good appetite during the period. Both the serum Na/K ratio and the plasma glucose level were maintained at a certain value after bilateral adrenalectomy. 6) In order to prolong the lifespan of some animals, a few times more than the usual maintenance dose of DOCA (5mg/head/day) was re-quired. 2) When the plasma cortisol concentrations were determined by the RIA method in the 23 animals who had survived over 2 months after bilateral adrenalectomy, the levels in 8 animals were relatively high, i. e., higher than 1.0ng/ml, and indeed in two of them the levels increased twofold over the controls after ACTH injection. It seems that some adrenal tissue remained after the operation and/or it was regenerated later with resultant cortisol secretion. These ob-servations show that we can successfully perform adrenalectomy on sheep by using our surgical procedure. It is recommended that the adrenalectomized sheep should be evaluated before they are used for experiments, based on highly sensitive methods of determining the plasma cortisol level.View full abstractDownload PDF (876K) -
Masatoshi IZUMIMOT, Ryosei IWAHARA, Hiroyuki MIURA1982 Volume 53 Issue 7 Pages 474-479
Published: July 25, 1982
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSEffects of anaerobic packaging on heme-pigment and lipid oxidations were investigated. Heme-pigment oxidation causes undesirable off-color and lipid oxidation produces off-odor. Sam-ples of ground beef were stored in anaerobic packages with a deoxygenizing agent, and compared with air packaged samples. Oxygen concentration rapidly decreased to 1% after packaging with an agent. In air packages, oxygen concentration gradually decreased rom 20% at the start to 1% at 10days with an increase in the carbon dioxide. The color of the samples corresponded o the proportions of met-form heme-pigment, i. e. anaerobic packaged samples demonstrated greater color stability than air packaged samples. At 10 days, the proportions of met-form hemepigment in the air packaged samples increased to almost 80%. However, the met-forms in the naerobic packaged samples decreased to 30% after first increasing to 90% at 1 day. Conseuently, it was confirmed that heme-pigment oxidation was inhibited by deoxygenation in the package. Thiobarbituric acid number in the anaerobic packaged samples was 30%iower than in the air packaged samples. Thus inhibition of lipid oxidation was found to have taken place. In sensory evaluation of cooked samples after 10 days' storage, sensory off-odor was not detected in anaerobic packaged samples, but detected in air packaged samples.View full abstractDownload PDF (409K) -
Yoshiaki MINAT, Yutaka TOYODA1982 Volume 53 Issue 7 Pages 480-487
Published: July 25, 1982
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSPorcine oocyte-cumulus complexes were cultured in various media in order to induce cumulus expansion and maturation division in vitro. When the complexes were cultured in the basic medium (modified Krebs-Ringer bicarbonate medium), 83.1% of oocytes resumed maturation division and 63.5% reached metaphase II in 48-54 hr, but cumulus expansion did not occur. Resumption of maturation division was not affected by the addition of only 10 i. u./ml PMSG or hCG, but was distinctly inhibited by the ad- dition of 1 or 5% fetal calf serum (FCS). Expansion of cumulus mass did not take place in the medium supplemented with either FCS or gonadotrophin. By contrast, in the medium containing both 1% FCS and gonadotrophin (10 i. u. PMSG or hCG), cumulus expansion was induced in high percentages (86.0-89.1%) and 67.5-78.8% of oocytes reached metaphase II. The proportion of oocytes reaching metaphase II was reduced when 5% FCS was added together with PMSG or hCG. Ultrafiltrated fraction of FCS (molecular weight<10, 000) exerted similar effects as whole FCS. It is suggested that gonadotrophin promotes both germinal vesicle breakdown and cumulus expansion in the presence of low molecular component (s) of FCS.View full abstractDownload PDF (947K) -
KAZUAKI TAKENOUCHI1982 Volume 53 Issue 7 Pages 488-496
Published: July 25, 1982
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSThe thermal degradation of hide collagen and tanned hide collagen was investigated by means of differential thermal analysis and infrared analysis. The hide collagen began to decompose at about 220°C and decomposed almost completely at around400°C in both air and nitrogen gas atmosphere. The weight losses due to the decomposition were near 70 percent of dry material in both air and nitrogen gas. The tanned hide collagen began to decompose at higher temperature in both air and nitrogen gas than the untanned hide collagen. But the combustion of the tanned hide collagen oc-curred drastically in air. TG-DTA curves of the tanned hide collagen were different, dependent on the content and kind of chromium complexes. It seems that sulfato chro-mium complexes increase the thermal stability of collagen more greatly than hydroxo chromium complexes.View full abstractDownload PDF (524K) -
YOKOHAMA M., K. MOGI, T. HOSODA1982 Volume 53 Issue 7 Pages 497-498
Published: July 25, 1982
Released on J-STAGE: March 10, 2008
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Hisao ITABASHI, Takeru KOBAYASHI, Noriaki YAMAGISHI, Hiroaki SHISHIDO1982 Volume 53 Issue 7 Pages 499-502
Published: July 25, 1982
Released on J-STAGE: March 10, 2008
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Eimei SATO, Takehiko ISHIBASHI1982 Volume 53 Issue 7 Pages 503-506
Published: July 25, 1982
Released on J-STAGE: March 10, 2008
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Koji ASHIZAWA, Keizo OKAUCH, Hisayoshi NISHIYAMA1982 Volume 53 Issue 7 Pages 507-509
Published: July 25, 1982
Released on J-STAGE: March 10, 2008
JOURNAL FREE ACCESSDownload PDF (176K)
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