日本畜産学会報
Online ISSN : 1880-8255
Print ISSN : 1346-907X
ISSN-L : 1880-8255
72 巻, 1 号
選択された号の論文の10件中1~10を表示しています
  • Yutaka NAKAI
    2001 年 72 巻 1 号 p. 1-13
    発行日: 2001/01/25
    公開日: 2008/03/10
    ジャーナル フリー
  • Koji KASAI, Akihisa IZUMO, Toshio INABA, Tsutomu SAWADA
    2001 年 72 巻 1 号 p. 14-18
    発行日: 2001/01/25
    公開日: 2008/03/10
    ジャーナル フリー
    Potential of a new sperm quality test, "In vitro sperm-egg interaction assay", for evaluating the fertilizing ability of spermatozoa from individual drakes was examined. The inner perivitelline layer (IPVL) separated from egg yolk was incubated with spermatozoa from individual drakes, and the number of holes formed on the IPVL by sperm hydrolysis in vitro were counted. At the same time, the sperm samples were subjected to motility, viability and fertility tests. The number of holes hydrolyzed on the IPVL in vitro by spermatozoa showed significant difference among the 6 males tested. The number of holes in the IPVL was highly correlated with the fertility of the eggs laid by inseminated females (r=0.90, P<0.001). On the other hand, sperm motility and viability varied little among the tested males, although the results of the fertility test for the same males varied with individuals (P<0.05). These results suggest that the in vitro sperm-egg interaction assay can describe the difference in the fertilizing ability of spermatozoa from individual drakes more clearly than conventional in vitro tests do. This new technique is considered to be applicable to the preliminary screening of the candidate males in the selection of breeder drakes.
  • Chisato HARADA, Yoshiko SAITO, Yutaka NAKAMURA, Hajime MINATO
    2001 年 72 巻 1 号 p. 19-25
    発行日: 2001/01/25
    公開日: 2008/03/10
    ジャーナル フリー
    In situ disappearance of cell wall constituents of the untreated and NaOH-treated rice straw was examined in detail. In situ cellulose digestion of rice straw was significantly improved by treatment with 4% NaOH. Hemicellulose in the cell walls of rice straw abundantly contained galactose and xylose, followed by arabinose, glucose, and uronic acids. In situ digestion of xylose residues in hemicellulose of the straw was inferior to that of other monosaccharides, but that of the straw was remarkably improved by the NaOH-treatment. The same amount of guaiacyl- and syringyl- lignins was released from the untreated rice straw during in situ digestion. On the other hand, more abundant syringyl-lignin than guaiacyl-lignin was released from NaOH-treated rice straw during the in situ digestion. In situ disappearance of ester-linked p-coumaric and ferulic acids from the untreated rice straw was larger than that of the ether-linked acids. While, in situ disappearance of ether-linked p-coumaric and ferulic acids from the NaOH-treated rice straw was larger than that of the ester-linked acids. The results obtained in this study suggest that the partial destruction of a network structure built up from hemicellulosic polysaccharides and lignin in rice straw cell walls by NaOH-treatment makes it easy for the access of cellulolytic microorganisms and their cellulases to cellulose and accelerates the in situ cellulose digestion of rice straw.
  • Toshimasa SAKAI, Masanori FUJITA, Shuichi FURUSAWA, Kei ICHISHIMA, Sad ...
    2001 年 72 巻 1 号 p. 26-31
    発行日: 2001/01/25
    公開日: 2008/03/10
    ジャーナル フリー
    Consecutive two experiments were conducted to investigate the daily changes of arterial and venous catecholamine concentrations and the response of those to immobilization (IM) in laying hens. In experiment 1, blood samples were collected from right femoral artery and left wing vein at 0800, 1400, 2000 and 0200h. Both of arterial and venous plasma norepinephrine (NE) concentrations tended to be high in the light and low in the dark period, but were not so in epinephrine (E). In experiment 2, blood samples were collected from right femoral artery and left wing vein at 6h (1400) and 24h (0800) after the start of IM. Both of arterial and venous plasma NE concentrations increased significantly (P<0.01 and P<0.01, respectively) at 6h after the start of IM, being continued till 24h of IM. Venous plasma E concentration increased significantly at 6 and 24h (P<0.05 and P<0.05, respectively) of IM. No differences of plasma NE and/or E concentrations were observed between the artery and the vein after the start of IM. These results suggest that there would be no difference in the daily changes of plasma CA concentration and the response of plasma CA concentration to immobilization between the venous plasma and the arterial plasma.
  • Shotaro NISHIMURA, Kentaro IKEDA, Kaoru OKANO, Takafumi GOTOH, Shoji T ...
    2001 年 72 巻 1 号 p. 32-38
    発行日: 2001/01/25
    公開日: 2008/03/10
    ジャーナル フリー
    In the goat adenohypophysis, percentages of somatotrophs, mammotrophs and somatomammotrophs were compared between the lactating females and the post-lactation immunohistochemically. The pituitary weight was significantly different between the lactating females (0.65±0.10g) and the past-lactation (0.38±0.07g). Except for zona tuberalis, mammotrophs appeared at significantly larger percentage in the lactating females (26.9%) than in the post-lactation (10.1%) and conversely somatomammotrophs in the post-lactation (28.1%) than in the other (16.0%) at 5% level. The percentage of somatotrophs increased only in the caudal region of the pars distalis but immunonegative cells did not show significant differences after the cessation of lactation. From these results, it was suggested that a part of mammotrophs might transform into somatomammotrophs after cessation of lactation in the female goat adenohypophysis.
  • Mariko TERASHIMA, Keiji YOSHIMURA, Tetsuo IMAI, Daiki HOZAN, Yasuhiro ...
    2001 年 72 巻 1 号 p. 39-45
    発行日: 2001/01/25
    公開日: 2008/03/10
    ジャーナル フリー
    Light deterioration of hair in mink fur was evaluated by sensory evaluation, color difference measurement, scanning electron microscope (SEM) observation, fourier transform infrared (FTIR) measurement and chemical analysis of extracted protein, aiming to have an insight into the practical aspect of quality examination by summarizing these evaluation. As a result of the sensory test on the deterioration of hair in irradiated mink fur, deterioration in smoothness and straightness of guard hair was recognized within the irradiation time of 96h. In instrumental examination, a significant deterioration was detected in color difference, SEM picture, FTIR spectrum and analyses of extracted proteins within the irradiation time of 96h. The degree of deterioration detected was generally proportional to the irradiation time. It was recognized that examination items adopted in this study were meaningful for the evaluation of light deterioration of hair in mink fur. Furthermore, the introduction of FTIR measurement and chemical analyses of extracted proteins enabled to detect such a light deterioration that can't be detected by sensory test.
  • Minoru YAMANOUE, Ryuji KIMURA, Yasunari AKAMATSU, Takahide OKAYAMA, Ak ...
    2001 年 72 巻 1 号 p. 46-53
    発行日: 2001/01/25
    公開日: 2008/03/10
    ジャーナル フリー
    It is known that bovine muscles stored before the rigor mortis in low temperature cause the cold shortening. This study was carried out in order to clarify the relation of weakening of myofibrillar structure during storage on meat tenderization of cold shortened bovine semimembranous muscle. The muscle was excised immediately after slaughter from the dressed carcass and divided into muscle blocks, and then stored antiseptically at 4°C. The muscle restrained for two days on the carcass was excised on day 3 postmortem and similarly treated. The value of breaking strength and elasticity of both muscles gradually decreased with the postmortem time. However, the values of both breaking strength and elasticity in the excised muscle decreased but less than the restrained muscle, showing that the cold shortening developed in the immediately excised muscle. Measurement on the weakening of the myofibrillar structures of the cold shortened muscle showed that the weakening of the Z-disks progressed linearly after 3 days postmortem, agreeing with decline of the stiffness of the muscle. The weakening of the interaction between actin and myosin that affects sarcomere length hardly occurred throughout the storage time. And, the degradation of α-connectin rapidly progressed after 4 days postmortem. These results suggest that the weakening of the Z-disks and the degradation of connectin filaments were related to the tenderization of uncooked cold shortened bovine muscle during postmortem storage.
  • Manabu KAWASE, Hideo HASHIMOTO, Masataka HOSODA, Hirotsugu MORITA, Aki ...
    2001 年 72 巻 1 号 p. 54-62
    発行日: 2001/01/25
    公開日: 2008/03/10
    ジャーナル フリー
    The serum cholesterol lowering action of fermented milk with Streptococcus thermophilus TMC 1543 (1543FM), the mechanism behind the cholesterol lowering activity and the active factor in animal experiments were investigated using rats. The serum total cholesterol level of the group fed 1543FM was significantly lower than that of the control group (P<0.05). However, there were no serum cholesterol lowering activities in fermented milks with the other two lactic acid bacteria. The serum total cholesterol concentration of the group fed low-molecular-weight fraction (LMF) from 1543 FM was significantly lower than that of the control group (P<0.05). High-molecular-weight fraction (HMF) also tended to lower serum cholesterol level in the present animal study. The amounts of excretion of both total bile acid and neutral sterol in feces of the 1543FM group increased significantly in comparison with the control group (P<0.05). These results suggest that the mechanism behind serum cholesterol lowering activity of 1543FM would be attributable to the promotion of excretion of bile acid and neutral steroid through feces, and probably the low-molecular material in LMF.
  • Shin-ichi TAKENOYAMA, Satoshi KAWAHARA, Michio MUGURUMA, Hisashi MURAT ...
    2001 年 72 巻 1 号 p. 63-71
    発行日: 2001/01/25
    公開日: 2008/03/10
    ジャーナル フリー
    In this paper, the 9cis, 11trans conjugated linoleic acid (9c, 11t CLA) contents of commercially available meat products and cheeses were determined using our developed method for measuring 9c, 11t CLA in order to obtain basic data about the 9c, 11t CLA content of animal products. The 9c, 11t CLA contents of domestic and imported meat products and cheeses and of ruminant and nonruminant meat products were compared. The mean 9c, 11t CLA content of the domestic meat products was not significantly different from that of the imported meat products. The 9c, 11t CLA content of meat products in which meat from ruminant animals was formulated as raw material was clearly higher than that of meat products from nonruminant animals. The imported cheese products had a higher 9c, 11t CLA content than the domestic products. The 9c, 11t CLA concentrations (mg/g lipid) of cheeses high in lipid content appeared to be relatively higher compared to those low in lipid content. Quantitative relationships between 9c, 11t CLA and other fatty acids in animal products originating from ruminant animals showed a negative correlation between 9c, 11t CLA and linoleic acid and a positive correlation between 9c, 11t CLA and vaccenic acid. The results of this study indicate that the large variations in 9c, 11t CLA contents of animal products may depend primarily on the materials from which they originate.
  • Kazuyoshi SUZUKI, Yasuo TANAKA, Takashi OSADA, Miyoko WAKI
    2001 年 72 巻 1 号 p. 72-79
    発行日: 2001/01/25
    公開日: 2008/03/10
    ジャーナル フリー
    In order to confirm the possibility of crystallization of P, Mg and Ca in swine wastewater, the concentration of P, Mg and Ca in such wastewater was determined by estimation and measurement of real swine wastewater, and artificial induction of a crystallizing reaction was attempted in swine wastewater. Based on estimates of the material flow of P, Mg and Ca in the average swine husbandry system in Japan, the concentrations of P, Mg and Ca in swine wastewater were calculated as 11.3mmol/l, 6.2mmol/l and 10.2mmol/l, respectively, when 100% of feces excreted was mixed into urine and washing water. It was shown that real swine wastewater also includes P, Mg and Ca in high concentrations, and that a considerable portion of PO43-, Mg and Ca naturally exist as crystal in swine wastewater of relatively high pH (7.7-8.7). It was confirmed that artificial crystallization of soluble PO43-, Mg and Ca can be done by adding NaOH to swine wastewater. Moreover, it was clarified that crystal in swine wastewater does not redissolve if a high pH condition is maintained. Crystal can be removed easily by sedimentation because of its high density. An artificial crystallizing process will contribute to a scale-free purification system for swine wastewater.
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