It was suggested by Prof. Maekawa for the first time that the organ specifity of an allergic reation was brought forth as the result of the participation in the allergic reaction of the substances showing special affinity to the organ involved. This has been proved experimentally by Prof. Maekawa's students, who used digitalis, nicotine and the phosphatide of the heart muscle as "cardio-tropic" substances are identical in their effect on the heart muscle. Two groups of rabbits were used to inquire after the "cardiotropic" action of nicotine and cardiac phosphatide. Group A was injected with the mixture of nicotine and ox serum for four days as the sensitization procedure and after three weeks the mixture of cardiac phosphatide and ox serum are injected for three days. Group B was treated similarly, but the sensitizing process was arranged contary to the case of group A. Body weight blood pressure, Arthus phenomenon, electrocardiogram and histological change were studied. The most important findings were obtained in the electrocardiographic and histological examination. As electrocardiographical findings we were able to point out tachycardia, lower T waves and ST transportation. As histological findings periarteritis were often seen in the myocardium of every rabbit. Cell infiltrations showed the speciality of the allergic change such as plasma cells, histiocytes, connective tissue cells and pseudo-eosinophile cells. Other organs showed no remarkable allergic changes. The experiment established similar effect of two "cardiotrope: substances in spite of their chemically very different structures. Nicotine, one kind of alkaloids, has a chamical nature like a base. Proteins which have amino and carboxyl bases combine with alkaline substances and produce some compounds like bases. So it is with a high probability that nicotine combines with protein. On the other hand lipoidprotein combinations are well known as lipoproteins. This expriment suggests that both nicotine and phosphatide of cardiac muscle bring the allergic reaction to the heart muscle in spite of their very different chemical structures.

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a) While carrying on the same experiments as described in the foregoing reports, we observed pathohistologically the hypertrophy of lymph nodes and the the picture of regeneration in them. Tonsils and spleen presented almost the same changes. b) Conclusion : The blood picture of rabbits, sensitzed and reinjected with mixture of human tonsil phosphatide and oxen sera, revealed an intense lymphocytosis with the presence of lymphoblasts, mitosis, young large or medium-sized lymphocytes. The changes of the lymphatic tissues were observed. Histologically, therefore, it is concluded that the lymphocytosis is probably due to the production of lymphatic cells, but not the mobilization.

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According to Prof. Maekawa's "Chain-doublets" theory, polarization of the border membran plays an important role for St and T variations. Special variations of ST & T in clinical electrocardiograms, for example, those of myocardial infarction, pericarditis and digitalis effect, should be analyzed and interpreted on the consideration of polarization of epicardium or endocardium. Analysis of local lead electrocardiograms certified that local injury of epicardium causes infarction pattern and entire injury brings pericarditis pattern, and on the other hand entire endocardial injury results in digitalis pattern. (1) Myocardial Infarction : As well known facts, curves obtained in the lead point that face the epicardial surface of the infarct show usually QS pattern and elevated ST segment or inverted T wave. The former is the sign of abnormal stimulus distribution in the infarct area and the latter is the consequence of local epicardial injury. Esophageal lead electrocardiograms in the anterior infarction show depressed ST segment not only in the endocardial side (in the auricular and supra-auricular site), but in the epicardial side (in the ventricular site). This fact indicates the existence of anterior infarction indirectly and simultaneously the absence of posterior infarction. In the posterior infarction the esophageal leads may show directly infarct curves but chest leads may not usually show the indirect infarct pattern i.e. negative ST displacement. Therefore, exacter diagnosis of infarct location should be performed by means of precordial and esophageal leads as the author's cases indicate. (2) Pericarditis and epicardial Haemorrhage : Pericarditis pattern is characterized by ST, T variation and low voltage and classified to 4 phases by Holzmann. The author's cases showed pattern of III or II phase. These local lead electrocardiograms showed inversed T wave in epicardial side i.e. in chest leads and esophageal leads at the ventricular hight, and positive T wave in endocardial side i.e. in esophageal leads at the auricular and supra-auricular hight, and moreover analysis of stimulus arrival time gave always norma values. A patient with normal electro cardiographic pattern, diagnosed as subarachnoid haemorrhage, showed inversed T waves in I and precordial leads, without QRS variation and cardiac symptoms, after about 5 weeks, unexpectedly. At that time epicardial haemorrhage was suspected and after about one month she died of second subarachnoid haemarrhage. An autopsy showed scattered epicardial haemorrhage and no myocardial and endocardial lesion. the author assert that these inversed T waves without QRS variation derive from excessive repolarization of epicard after lesion and myocardial damage is secondary. (3) Digitalis electrocardiogram : ST, T depression due to digitalis effect is not clearly elucidated even now. Local lead electrocardiograms in the author's cases showed St, T depression in epicardial side and ST,T elevation in endocardial side after digitalization. So it is concluded that constant and incomplete depolarization of entire endocardium gives rise to the digitalis pattern, because polarization membrane injury due to digitalis may occur rather chiefly in the endocardium than in the epicardium.

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The author investigated the change of venous wave or auricular wave before and after artificial Pneumothoraces in patients of pulmonary tuberculosis or in dogs, and obtained following results. (1) Depth of X-valley in venous wave decreased after Pneumothorax and increased after discharge of air from pleural cavity. This was also the case in S_m-wave of auricular wave. (2) When large amount of air was introduced continuously or cumulatively, depth of S_m-wave decreased not in linear but in parabolical relation to the amount of air introduced. (3) Both before and after Pneumothorax, when preceding cardiac cycle was long, S_m-wave or X-valley denotes the suction-power of heart (s. 1st report), these experimental results show that suction-mechanism of heart is closely related to pulmonary suction-mechanism.

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