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Volume 53, Issue 12
Displaying 1-25 of 25 articles from this issue
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Elena Ramírez, Marisa Cabeza, Eugene Bratoeff, Ivonne Heuze, V& ...2005 Volume 53 Issue 12 Pages 1515-1518
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSIn this study we report the synthesis and pharmacological evaluation of four new progesterone derivatives; 17α-hydroxy-16β-methylpregna-4,6-diene-3,20-dione 12, 17α-cyclopropylcarbonyloxy-16β-methylpregna-4,6-diene-3,20-dione 13, 17α-cyclobutylcarbonyloxy-16β-methylpregna-4,6-diene-3,20-dione 14, 17α-acetoxy-16β-methylpregna-4,6-diene-3,20-dione 15 and the pregnatriene compound 17α-cyclobutylcarbonyloxy-16β-methylpregna-1,4,6-triene-3,20-dione 16. The pharmacological effect of these compounds was determined in vivo as well as in vitro. The evaluation in vivo was carried out on gonadectomized male hamsters that were injected subcutaneously daily with testosterone (T) and/or finasteride, or with the novel compounds. At the end of the treatments the animals were sacrificed and the prostates were weighed. It was observed that when testosterone (T) and finasteride or compounds 12—16 were injected together, the weight of the prostate decreased significantly as compared to that of the testosterone-treated animals. The 5α-reductase inhibitory activity was evaluated in vitro using human prostate homogenates. These experiments showed the following IC50 values: compound 12 (alcohol at C-17) 1.2×10−6 M, 13 (cyclopropyl substituent at C-17) 7.9×10−10 M, 14 (cyclobutyl substituent) 3.2×10−8 M, 15 (acetoxy substituent) 6.3×10−11 M and 16 (cyclobutyl substituent) 3.9×10−6 M. It is evident from these data that when the size of the substituent at C-17 is decreased, the 5α-reductase inhibitory activity increases. Apparently, in this biological model, the 5α-reductase inhibitory activity depends upon the steric effect of the substituent at C-17. However, the free alcohol 12 showed much lower 5α-reductase inhibitory activity.View full abstractDownload PDF (150K) -
Masanori Kuroyanagi, Mari Shimomae, Yasuo Nagashima, Norio Muto, Takur ...2005 Volume 53 Issue 12 Pages 1519-1523
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSIn the course of research on the bioactive constituents of woody plants in the Cyugoku area of Japan, a methanol extract of the leaves of Alnus japonica were found to have strong antioxidative activity. Ethyl acetate soluble and n-buthanol soluble fractions of the methanol extract had a potent antioxidative effect. Both fractions were purified by silica gel column chromatography and HPLC using an ODS column to give four new diarylheptanoids along with known diarylheptanoids and flavonoids. These new compounds were elucidated to be 7-(3,4-dihydroxyphenyl)-5-hydroxy-1-(4-hydroxyphenyl)-3-heptanone-5-O-β-D-xylopyranoside (1), 1-(3,4-dihydroxyphenyl)-5-hydroxy-7-(4-hydroxyphenyl)-3-heptanone-5-O-β-D-xylopyranoside (2), 1,7-bis-(3,4-dihydroxyphenyl)-5-hydroxy-3-heptanone-5-O-[2-(2-methylbutenoyl)]-β-D-xylopyranoside (3) and 1,7-bis-(3,4-dihydroxyphenyl)-5-methoxy-3-heptanone (4) using spectral methods and especially 1H-, 13C-NMR and 2D-NMR measurements. The isolated compounds including their main constituent, oregonin (5), were tested for antioxidative activity. Some of these compounds having two catechol structures showed potent antioxidative activity. Compounds having one catechol structure showed moderate antioxidative activity, but a peracetate of 5 having no catechol structure exhibited no antioxidative activity. Thus the catechol structure of the diarylheptanoids is indispensable for antioxidative activity.View full abstractDownload PDF (146K) -
Joaquín González Marrero, Lucía San Andrés ...2005 Volume 53 Issue 12 Pages 1524-1529
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSThe known diterpenes 12,16-epoxycarnosol (2), isotanshinone II (6), and (+)-neocryptotanshinone (8) were obtained by partial synthesis from 16-hydroxycarnosol (1), a C-16 hydroxylated abietatriene diterpene isolated in relative abundance from the aerial part of Salvia mellifera GREENE. The physical and spectroscopic data of these semisynthetic diterpenes were identical to those given for the natural ones in the literature. These abietane diterpenes have very interesting biological activities and the semisynthetic approach described here represents an alternative to obtain them from other major diterpenes isolated from Salvia species. Additionally, seven new semisynthetic diterpene analogues, 11,14-dioxo-12,16-epoxy-8,12-abietadien-20,7β-olide (3), 11,14-dioxo-12,16-epoxy-8,12,15(16)-abietatrien-20,7β-olide (4), 15,16-didehydro-12,16-epoxycarnosol (5), 1-oxoisotanshinone II (7), 16-hydroxycolumbaridione (9), 12,16-diacetoxycolumbaridione (10), and 14-methoxy-12,16-epoxycarnosol (13), were obtained from 1. The structures of the new compounds were established based on their spectroscopic data.View full abstractDownload PDF (177K) -
Natesan Subramanian, Saroj Kumar Ghosal, Asis Acharya, Satya Priya Mou ...2005 Volume 53 Issue 12 Pages 1530-1535
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSThe phase behavior of the system isopropyl myristate/medium-chain glyceride/polysorbate 80/water forming both w/o and o/w microemulsions has been studied to develop microemulsion comprising of pharmaceutical excipients. The pseudo-ternary phase diagrams with large monophasic zones and gel formation were realized and several compositions were identified in the phase diagram for rheological, dynamic light scattering (DLS) and calorimetric measurements. The identified systems at different temperatures behaved as Newtonian fluid and the activation parameters for their viscous flow were evaluated. From DLS measurements, hydrodynamic diameter, polydispersity and diffusion coefficient of the microheterogeneous dispersions were determined. The free energy, enthalpy and entropy of solution of the w/o and o/w microemulsions were determined from calorimetric measurements.View full abstractDownload PDF (246K) -
Jinichi Fukami, Asuka Ozawa, Yasuo Yoshihashi, Etsuo Yonemochi, Katsuh ...2005 Volume 53 Issue 12 Pages 1536-1539
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSA fast disintegrating compressed tablet was formulated using amino acids, such as L-lysine HCl, L-alanine, glycine and L-tyrosine as disintegration accelerator. The tablets having the hardness of about 4 kgf were prepared and the effect of amino acids on the wetting time and disintegration time in the oral cavity of tablets was examined on the basis of surface free energy of amino acids. The wetting time of the tablets increased in the order of L-lysine HCl, L-alanine, glycine and L-tyrosine, whereas the disintegration time in the oral cavity of the tablets increased in the order of L-alanine, glycine, L-lysine HCl and L-tyrosine. These behaviors were well analyzed by the introduction of surface free energy. When the polar component of amino acid was large value or the dispersion component was small value, faster wetting of tablet was observed. When the dispersion component of amino acid was large value or the dispersion component was small value, faster disintegration of tablet was observed, expect of L-tyrosine tablet. The fast disintegration of tablets was explained by the theory presented by Matsumaru.View full abstractDownload PDF (206K) -
Jean-Bernard Bongui, Abdelhakim Elomri, Dominique Cahard, Franç ...2005 Volume 53 Issue 12 Pages 1540-1546
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSCondensation of 1-bromo-2-naphthalenecarboxylic acid (9) with 7-methoxy-2,2-dimethyl-2H-1-benzopyran-5-ylamine (13) followed by acid-mediated cyclization afforded 6-methoxy-3,3-dimethyl-3,14-dihydro-7H-benzo[c]pyrano[3,2-h]acridin-7-one (15), which was further methylated into 6-methoxy-3,3,14-trimethyl-3,14-dihydro-7H-benzo[c]pyrano[3,2-h]acridin-7-one (benzo[c]acronycine) (3) and 6,7-dimethoxy-3,3-dimethyl-3H-benzo[c]pyrano[3,2-h]acridine (4). Osmium tetroxide oxidation of 15 gave the (±)-cis-diol 16, which afforded the benzopyranoacridine and benzopyranoacridone esters 17—22 upon acylation. Condensation of 9 with suitable aminoquinolines 23—25 afforded the carboxylic naphthylquinolylamines 26—28. Cyclization gave the corresponding naphtho[1,2-b][1,10]-phenanthrolin-7(14H)-ones 29 and 30, and naphtho[1,2-b][1,7]-phenanthrolin-7(14H)-one 31, which were subsequently N-methylated to the desired 14-methylnaphtho[1,2-b][1,10] and [1,7]-phenanthrolinones 6, 7, and 8. Benzo[c]pyrano[3,2-h]acridin-7-one derivatives 3, 16, and 22 displayed cytotoxic activities within the same range of magnitude as acronycine itself, whereas 7-alkoxybenzo[c]pyrano[3,2-h]acridine and 7-acyloxybenzo[c]pyrano[3,2-h]acridine derivatives 4 and 17—21 were less active when tested against L1210 murine leukemia cells in vitro. Naphthophenanthrolinones 6—8 were devoid of significant antiproliferative activity, but compounds 29—31 bearing no substituent on the nitrogen atom at position 14 were more potent.View full abstractDownload PDF (232K) -
Yoshinobu Sato, Takumi Okamoto, Satoru Watano2005 Volume 53 Issue 12 Pages 1547-1550
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSScale-up of wet granulation in a vertical high shear mixer was conducted. Pharmaceutical excipient mixtures composed of lactose, cornstarch and micro-crystalline cellulose, and hydroxypropylecellulose as a binder were mixed together and then granulated with purified water under various operating conditions and vessel scales. Torque of agitator shaft was continuously measured and then agitation power per unit vessel volume was calculated. The agitation power per unit vessel volume showed a good correlation with physical properties of obtained granules, such as mass median diameter, strength and compressibility. This implied that the scale-up characteristics could be well analyzed by means of the agitation power per unit vessel volume. In addition, the effects of agitator tip speed and Froude number on the agitation power per unit vessel volume were investigated. The results showed that the agitation power per unit vessel volume was well characterized by the tip speed rather than the Froude number. This meant that the granule growth mainly progressed by the shear stress from the agitator blade. Dynamic characteristics of high shear granulation were also discussed here.View full abstractDownload PDF (124K) -
Masanori Inagaki, Tomofumi Miyamoto, Ryuichi Isobe, Ryuichi Higuchi2005 Volume 53 Issue 12 Pages 1551-1554
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSA ganglioside molecular species, LLG-5 (1), has been obtained from the water soluble lipid fraction of the CHCl3/MeOH extract of the starfish Linckia laevigata. On the basis of chemical and spectroscopic findings, the structure of 1 has been elucidated. Negative ion FAB-MS provided important information both on the structure of the sugar moiety and on the molecular mass of the ganglioside. 1 is a new ganglioside molecular species possessing a 2→11 linked linear-type trisialosyl moiety. Moreover, 1 exhibited neuritogenic activity in rat pheochromocytoma PC-12 cells in the presence of nerve growth factor.View full abstractDownload PDF (183K) -
Kazuki Toyoda, Yasunori Yaoita, Masao Kikuchi2005 Volume 53 Issue 12 Pages 1555-1558
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSThree new dimeric benzofuran derivatives, ligulacephalins A (1), B (2) and C (3), were isolated from the roots of Ligularia stenocephala MATSUM. ET KOIDZ. (Compositae) together with three known compounds, 5,6-dimethoxy-2-isopropenylbenzofuran (4), euparin (5) and (R)-(−)-hydroxytremetone (6). The structures of the new compounds were determined by spectroscopic evidence. The chiral HPLC analysis demonstrated that 1—3 occurred as a racemate. The absolute configurations of each enantiomer from 1—3 were elucidated on the basis of circular dichroism (CD) data.View full abstractDownload PDF (233K) -
Masayuki Yoshikawa, Toshio Morikawa, Ning Li, Akifumi Nagatomo, Xian L ...2005 Volume 53 Issue 12 Pages 1559-1564
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSThe saponin fraction from the seeds of the tea plant [Camellia sinensis (L.) O. KUNTZE (Theaceae)] was found to exhibit potent protective effects on ethanol- and indomethacin-induced gastric mucosal lesions in rats. Five new triterpene saponins, theasaponins E3 (1), E4 (2), E5 (3), E6 (4), and E7 (5), were isolated together with 11 known saponins from the saponin fraction. The chemical structures of 1—5 were elucidated on the basis of chemical and physicochemical evidence. Among the isolated saponins, theasaponins E1 (6), E2 (7), and E5 (3) and assamsaponin C (10) showed an inhibitory effect on ethanol-induced gastric mucosal lesions at a dose of 5.0 mg/kg, p.o. and their activities were stronger than that of omeplazole. With regard to the structure–activity relationships of theasaponins, the following structural requirements for a protective effect on ethanol-induced gastric lesions were suggested; 1) the 21- and/or 22-acyl groups are essential for the activity, 2) acetylation of the 16-hydroxyl group reduce the activity.View full abstractDownload PDF (163K) -
Andria Agusta, Shoji Maehara, Kazuyoshi Ohashi, Partomuan Simanjuntak, ...2005 Volume 53 Issue 12 Pages 1565-1569
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSThe microbial transformation of five flavans (1—5) by endophytic fungi isolated from the tea plant Camellia sinensis was investigated. It was found that the endophytic filamentous fungus Diaporthe sp. oxidized stereoselectively at C-4 position of (+)-catechin (1) and (−)-epicatechin (2) to give the correspondent 3,4-cis-dihydroxyflavan derivatives (6, 10), respectively. (−)-Epicatechin 3-O-gallate (3) and (−)-epigallocatechin 3-O-gallate (4) were also oxidized by the fungus into 3,4-dihydroxyflavan derivatives (10, 12) via (−)-epicatechin (2) and (−)-epigallocatechin (11), respectively. Meanwhile, (−)-gallocatechin 3-O-gallate (5), (−)-catechin (ent-1) and (+)-epicatechin (ent-2), which possess a 2S-phenyl substitution, resisted the biotransformation.View full abstractDownload PDF (209K) -
Kenji Hoshi, Junko Kawakami, Mitiko Kumagai, Sanae Kasahara, Noriaki N ...2005 Volume 53 Issue 12 Pages 1570-1574
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSThyroid function diagnosis is an important classification problem, and we made reanalysis of the human thyroid data, which had been analyzed by the multivariate analysis, by the two notable neural networks. One is the self-organizing map approach which clusters the patients and displays visually a characteristic of the distribution according to laboratory tests. We found that self-organizing map (SOM) consists of three well separated clusters corresponding to hyperthyroid, hypothyroid and normal, and more detailed information for patients is obtained from the position in the map. Besides, the missing value SOM which we had introduced to investigate QSAR problem turned out to be also useful in treating such classification problem. We estimated the classification rates of thyroid disease using Bayesian regularized neural network (BRNN) and found that its prediction accuracy is better than multivariate analysis. Automatic relevance determination (ARD) method of BRNN was surely verified to be effective by the direct calculation of classification rates using BRNN without ARD for all possible combinations of laboratory tests.View full abstractDownload PDF (651K)
Notes
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Gang Xu, Li-Yan Peng, Yu Zhao, Xiao-Li Li, Lin Tu, Qin-Shi Zhao, Han-D ...2005 Volume 53 Issue 12 Pages 1575-1576
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSTwo new icetexane diterpenoids, przewalskin C, D (1, 2), together with sixteen known diterpenoids, were isolated from Salvia przewalskii. Eight of known ones (compounds 3, 12—18) were reported firstly from this plant. To the best of our knowledge, it's the first report of icetexane diterpenoids from this plant. The identification and structural elucidation of these compounds were based on 1D- and 2D-NMR spectral data analysis.View full abstractDownload PDF (70K) -
Ayumi Ohsaki, Yoshiko Kishimoto, Takahiko Isobe, Yoshiyasu Fukuyama2005 Volume 53 Issue 12 Pages 1577-1579
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSTwo new labdane diterpenoids, 15β-methoxyfaciculatin (1) and 15α-methoxyfaciculatin B (2), together with the previously known methoxynepetaefolin (3), were isolated from a methanol extract of the dried aerial parts of a Brazilian medicinal plant, Hyptis faciculata. Their structures were elucidated by analysis of spectroscopic data. Plausible biogenetic correlation between faciculatins and nepetaefolin is briefly discussed.View full abstractDownload PDF (165K) -
A New Acetylenic Compound from the Rhizomes of Atractylodes chinensis and Its Absolute ConfigurationYoichiro Nakai, Iwao Sakakibara, Kazuhiro Hirakura, Susumu Terabayashi ...2005 Volume 53 Issue 12 Pages 1580-1581
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSA new acetylenic compound “atractyloyne”, (3S,4E,6E,12E)-1-isovaleryloxy-tetradeca-4,6,12-triene-8,10-diyne-3,14-diol (1) was isolated from the rhizomes of Atractylodes chinensis (Compositae) together with a known compound (4E,6E,12E)-3-isovaleryloxy-tetradeca-4,6,12-triene-8,10-diyne-1,14-diol (2). These structures were determined on the basis of the spectroscopic data and chemical evidence, and the absolute configuration of 1 was established by the modified 2-methoxy-2-trifluoromethylphenylacetic acid (MTPA) method.View full abstractDownload PDF (77K) -
Ping Zhang, Zheng Cui, Yashu Liu, Dong Wang, Na Liu, Masayuki Yoshikaw ...2005 Volume 53 Issue 12 Pages 1582-1586
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSA simple and efficient HPLC method has been developed to evaluate the quality of traditional Chinese medicine toad venoms. The major bioactive ingredients, including 10 bufogenins and 4 indole alkaloids in the drug, were separated and quantified on a phenyl-hexyl column with a UV detector. A total of 27 toad venom samples from two species, Bufo bufo gargarizans CANTOR and Bufo melanostictus SCHNEIDER, from the different drug production regions of China, were analyzed. The chromatograms showed significant differences with respect to the samples from different origins. These toad venom samples can be distinctly classified into 4 groups by cluster analysis using the contents of the 14 main constituents, including toad venom samples from B. bufo gargarizans from north China, median China and south China, and samples from B. melanostictus from south China. Toad venom samples from B. bufo gargarizans from median China were considered to be of the highest quality.View full abstractDownload PDF (130K) -
Guoliang Chen, Wei Shan, Yingliang Wu, Lixiang Ren, Jinhua Dong, Zhizh ...2005 Volume 53 Issue 12 Pages 1587-1590
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSSeventeen novel resveratrol derivatives were synthesized. Their anti-inflammatory activities were tested on xylene-induced mouse ear edema. The pharmacological results showed that some compounds have potent anti-inflammatory activities.View full abstractDownload PDF (144K) -
Maryam Khoubnasabjafari, Abolghasem Jouyban, William Eugene Acree, Jr.2005 Volume 53 Issue 12 Pages 1591-1593
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSApplicability of a solution model for calculating salt's solubility in binary solvent mixtures was shown. The accuracy of the proposed model was evaluated by computing mean percentage deviation (MPD) employing available solubility data of electrolytes in binary solvents at various temperatures from the literature. The overall MPD (±S.D.) for correlation of solubility data was 4.7±5.1% and for prediction using model trained by a minimum number of experimental data points was 10.5±12.5%.View full abstractDownload PDF (107K) -
Dae Sik Jang, Jong Min Kim, Joo-Hwan Kim, Jin Sook Kim2005 Volume 53 Issue 12 Pages 1594-1596
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSFractionation of an EtOAc-soluble extract of the stems of Rumex japonicus led to the isolation of two new 24-norursane type triterpenoids, 2α,3α,19α-trihydroxy-24-norurs-4(23),12-dien-28-oic acid (1) and 4(R),23-epoxy-2α,3α,19α-trihydroxy-24-norurs-12-en-28-oic acid (2), along with the known compounds myrianthic acid (3), tormentic acid, and emodin. The structures of 1 and 2 were elucidated by spectroscopic methods, particularly by extensive 1D and 2D NMR studies.View full abstractDownload PDF (123K) -
Kohei Kamiya, Yohei Tanaka, Hanani Endang, Mansur Umar, Toshiko Satake2005 Volume 53 Issue 12 Pages 1597-1599
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSFrom the fruits of Morinda citrifolia L., one new anthraquinone, 5,15-O-dimethylmorindol, together with five known anthraquinones and one new iridoid, morindacin, together with two known iridoids, were isolated. Their structures were elucidated by analysis of spectroscopic data.View full abstractDownload PDF (99K) -
Phan Minh Giang, Phan Tong Son, Katsuyoshi Matsunami, Hideaki Otsuka2005 Volume 53 Issue 12 Pages 1600-1603
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSTwo new megastigmane glucosides, called excoecariosides A and B were isolated from the leaves of a medicinal Vietnamese plant, Excoecaria cochinchinensis LOUR. var. cochinchinensis (Euphorbiaceae) together with seven known compounds. Their structures were elucidated by spectroscopic analyses and chemical reactions including the modified Mosher's method.View full abstractDownload PDF (126K) -
Jiang Meng, Kelvin Sze-Yin Leung, Zhihong Jiang, Xiaoping Dong, Zhongz ...2005 Volume 53 Issue 12 Pages 1604-1609
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSA HPLC-DAD-MS fingerprint method of fresh Houttuynia cordata THUNB. was developed basing on the consistent chromatographic features among 11 batches of authentic samples. Major chemical components including phenolic compounds, flavones and alkaloids were simultaneously analyzed. Eleven common peaks in the fingerprint were chosen and identified by comparing their UV and ESI-MS data with the authentic compounds. The unique properties of this HPLC-DAD-MS fingerprint were successfully applied to analyze and differentiate samples from different geographical origins, processing methods and various medicinal parts of H. cordata. The results showed that these variations will give rise to differences in identities and/or abundance of chemical compounds, indicating that a comprehensive quality evaluation of those major ingredients in H. cordata is critical to assess and represent its overall quality.View full abstractDownload PDF (461K) -
Wei Yuan, Ziming Lu, Ying Liu, Chao Meng, Ke-di Cheng, Ping Zhu2005 Volume 53 Issue 12 Pages 1610-1612
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSThree new podocarpane-type diterpenoids, 3β,12-dihydroxy-13-methyl-6,8,11,13-podocarpatetraen (1), 3β,12-dihydroxy-13-methyl-5,8,11,13-podocarpatetraen-7-one (2), and 1-(7-hydroxy-2,6-dimethyl-1-naphthyl)-4-methyl-3-pentanone (3) (20(10→5)-abeo-4,5-seco-5(10),6,8,11,13-podocarpapentaen-3-one) were isolated from callus of Securinega suffruticosa. The structures were elucidated by spectroscopic methods. Compounds 1, 2, and 3 showed cytotoxic activity against five human cancer cell lines (A549, BEL 7402, BGC-823, HCT-8, A2780).View full abstractDownload PDF (107K) -
Xiu-Qin Li, Ai-Hua Song, Wen Li, Xiao-Hui Chen, Kai-Shun Bi2005 Volume 53 Issue 12 Pages 1613-1617
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSFatty acid of the root of the medicinal plant of Bupleurum Chinense DC in China has been investigated by gas chromatography combined with mass spectroscopy. After methyl-esterification, eight fatty acid compositions were identified by GC-MS. A simple and rapid determination of the fatty acid has been firstly developed by GC-FID. The derivatization condition was investigated in order to validate this method. Palmitic acid, palmitoleic acid, oleic acid and linoleic acid were analyzed simultaneously by internal standard method. The validity of method has been examined both experimentally with good recovery, intra-assay precisions and linearity. The quick and accurate method of capillary gas chromatography could be used for the analysis of the fatty acid from Bupleurum Chinense DC.View full abstractDownload PDF (124K)
Errata
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2005 Volume 53 Issue 12 Pages 1618
Published: 2005
Released on J-STAGE: December 01, 2005
JOURNAL FREE ACCESSDownload PDF (44K)
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