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Volume 53, Issue 6
Displaying 1-29 of 29 articles from this issue
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Regular Articles
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Xun Sun, You-Rong Duan, Qin He, Jiao Lu, Zhi-Rong Zhang2005 Volume 53 Issue 6 Pages 599-603
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSBiodegradable monomethoxy(polyethyleneglycol)–poly(lactide-co-glycolide)–monomethoxy(poly-ethyleneglycol) (PELGE) copolymers were synthesized by ring-opening polymerization to formulate plasmid DNA loaded nanoparticles. A double emusion method with polyvinyl alcohol as the emulsifier in the external aqueous phase was employed to prepare nanoparticles. The effects of monomethoxypoly(ethyleneglycol) (mPEG) segments in the polymer on particle size, zeta potential, encapsulation efficiency and in vitro release were investigated. It was found that the introduction of a certain amount of hydrophilic mPEG segments in the copolymer chains could improve the affinity of copolymer with plasmid DNA and enhance the emulsification ability of the copolymer. Thus DNA loaded nanoparticles with smaller particle sizes and higher encapsulation efficiencies were obtained by using PELGE copolymer as the matrix.View full abstractDownload PDF (492K) -
Mohammad Sohel Rana, Keietsu Tamagake2005 Volume 53 Issue 6 Pages 604-606
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSElectrochemical formation of ferryl porphyrin was examined by electrocatalytic oxidation of alkene by measuring luminol chemiluminescence using a flow-injection method. Emission was observed both below the reduction potential of FeIIITMPyP (−0.08 V at pH 11, −0.02 V at pH 7 and 0.15 V at pH 3) and above the oxidation potential (0.6 V at pH 11, 0.75 V at pH 7 and 1.1 V at pH 3). However, both anodic and cathodic emissions were inhibited significantly by the addition of alkene (cyclopent-2-ene-1-acetic acid) solutions downstream of the working electrode. Further, the spectra at both anodic and cathodic sides shifted to the longer wavelength (>424 nm) compared to the original spectrum of FeIIITMPyP (422 nm), which was not observed with the addition of alkene solution. Therefore, the results suggest that the electrochemically generated oxo-ferryl species have been engaged in catalytic oxidation of alkene before the flow reaches the observation cell.View full abstractDownload PDF (446K) -
Xiaodong Wang, Wenyuan Gao, Zhi Yao, Shaoyu Zhang, Yanwen Zhang, Yoshi ...2005 Volume 53 Issue 6 Pages 607-610
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSFive new sesquiterpenes, 1β-furanoyl-2β,3α,7α,8β,11-pentaacetoxy-4α,5α-dihydroxy-dihydroagarofuran (1), 1β,2β,3α,5α,7β,8β,11-heptaacetoxy-dihydroagarofuran (2), 1β-furanoyl-2β,3α,7α,8β,11-pentaacetoxy-5α-hydroxy-dihydroagarofuran (3), 1β,7β,8α-triacetoxy-2β-furanoyl-4α-hydroxy-11-isobutyryloxy-dihydroagarofuran (4), and 1β-nicotinoyl-2β,5α,7β-triacetoxy-4α-hydroxy-11-isobutyryloxy-8α-furanoyl-dihydroagarofuran (5), were isolated from the xylem of Tripterygium wilfordii, together with a known compound (6). Their structures were elucidated on the basis of spectroscopic studies. Compounds 2—5 showed significant immunosuppressive activities.View full abstractDownload PDF (108K) -
Sanjay Bajaj, Surinder Singh Sambi, Anil Kumar Madan2005 Volume 53 Issue 6 Pages 611-615
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSRelationship between topochemical indices and inhibition of CDK2/cyclin A by 3-aminopyrazoles was investigated using a data set comprising of 42 3-aminopyrazoles. Three topochemical indices—the Wiener's topochemical index – a distance based topochemical index, atomic molecular connectivity index – an adjacency based topochemical index and superadjacency topochemical index – an adjacency-cum-distance based topochemical index were used for the present investigations. The values of Wiener's topochemical index, atomic molecular connectivity index and superadjacency topochemical index for each of the 42 compounds comprising the data set were computed using an in-house computer program. Resultant data was subsequently analyzed and suitable models were developed after identification of the active ranges. Subsequently, a biological activity was assigned to each of the compounds using these models, which was then compared with the reported CDK2/cyclin A inhibitory activity. High accuracy of prediction ranging from 86 to 89% was observed using these models.View full abstractDownload PDF (83K) -
Kenneth Oben Eyong, Karsten Krohn, Hidayat Hussain, Gabriel Ngosong Fo ...2005 Volume 53 Issue 6 Pages 616-619
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSNewbouldiaquinone (1), a new naphthoquinone–anthraquinone coupled pigment and a new ceramide named newbouldiamide (2), have been isolated from Newbouldia laevis, besides the known compounds lapachol (3), canthic acid, oleanolic acid, 2-methyl-9,10-anthracenedione, 2-acetylfuro-1,4-naphthoquinone, 2,3-dimethoxy-1,4-benzoquinone, 2-(4-hydroxyphenyl)ethyl triacontanoate, β-sitosterol and β-sitosterol glucopyranoside. The structure elucidations of the isolated new compounds were performed on the basis of spectroscopic and chemical evidence. Preliminary studies showed that 1 is moderately antibacterial against Gram-positive Bacillus megaterium and that 3 has moderate herbicidal and antibacterial activities.View full abstractDownload PDF (129K) -
Mei-Ying Ning, Ying-Zhi Guo, Huai-Zhong Pan, He-Ming Yu, Zhong-Wei Gu2005 Volume 53 Issue 6 Pages 620-624
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSClotrimazole (CT)-containing proliposomes were prepared by penetrating an ethanol solution of CT and Egg phosphatidylcholine (PC) into microporous sorbitol particles, followed by vacuum evaporation of the solvent. As a result, CT proliposomes with free-flowing flowability were obtained. On contact with water, the proliposomes were rapidly converted into a liposomal dispersion, in which a certain amount of CT was entrapped by the liposomes. The result in scanning electronic micrograph confirmed the formation of liposomes structures from proliposomes, and the particles revealed round or ellipse. The ratio of drug to total lipid, ratio of PC to cholesterol and ratio of lipid to sorbitol affected the entrapment efficiency (EE%). The EE% of optimized formulation (CT 10 mg, 0.1 g total lipid, PC/CH ratio is 60 : 40 and 1 g sorbitol) in this investigation was 96.2±1.5%. The proliposomes system can provide sustaining release in simulated vaginal fluid at 37±1 °C for 24 h. In-vivo performance of blank proliposomes, a physical mixture of sorbitol and drug, clotrimazole proliposomes and commercial ointment formulation were evaluated using antifungal activity test. At 7 d post-dose, the c.f.u. of C. albicans decreased in proliposomes-treated groups than ointment and the physical mixture (t-Student, p<0.05). The results indicated that CT-containing vaginal proliposomes prolonged drug release and may increase amount of drug retention into the mucosa to result in more antifungal efficacy. In addition, CT-proliposomes did not affect the morphology of vaginal tissues. Therefore, the dosage form might be further developed for safe, convenient, and effective treatment of vaginal candidasis with reduced dosing interval.View full abstractDownload PDF (495K) -
Toshio Morikawa, Shin Ando, Hisashi Matsuda, Shinya Kataoka, Osamu Mur ...2005 Volume 53 Issue 6 Pages 625-630
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSThe 80% aqueous acetone extract from the rhizomes of Alpinia galanga showed nitric oxide (NO) production inhibitory activities in mouse peritoneal macrophages. From the aqueous acetone extract, three new 8–9′ linked neolignans, galanganal, galanganols A and B, and a sesquineolignan, galanganol C, were isolated together with nine known phenylpropanoids and p-hydroxybenzaldehyde. The structures of new neolignans were determined on the basis of physicochemical and chemical evidence. In addition, the inhibitory effects of the constituents from the rhizomes of A. galanga on NO production induced by lipopolysaccharide in mouse peritoneal macrophages were examined. Among them, galanganal (IC50=68 μM), galanganols B (88 μM) and C (33 μM), 1′S-1′-acetoxychavicol acetate (2.3 μM), 1′S-1′-acetoxyeugenol acetate (11 μM), trans-p-hydroxycinnamaldehyde (ca. 20 μM), trans-p-coumaryl alcohol (72 μM), and trans-p-coumaryl diacetate (19 μM) were found to show inhibitory activity.View full abstractDownload PDF (198K) -
Hongfei Liu, Shu Zhang, Shufang Nie, Xin Zhao, Xiyang Sun, Xingang Yan ...2005 Volume 53 Issue 6 Pages 631-633
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSMethylacrylic acid/styrene cross-linked with divinylbenzene is a novel pH-sensitive ion exchange resin. Microspheres of this resin were characterized by Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). The microspheres showed a pulsatile swelling behavior when the pH of the media changed. The pH-sensitive microspheres were loaded with salbutamol sulfate and the drug-release characteristics were studied under both simulated gastric and intestinal pH conditions. The results obtained showed that the drug release also depended on the pH of the release media.View full abstractDownload PDF (344K) -
Abolghasem Jouyban, Nora Yat Knork Chew, Hak-Kim Chan, Mohammad Sabour ...2005 Volume 53 Issue 6 Pages 634-637
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSOrganic solvents are amongst the most powerful solubilization agents for a large number of water-insoluble drugs. A number of equations has been reported for mathematical representation of solute solubility in mixed solvents. The question is then posed—is there a mathematical difference between these models? To address this point, it has been demonstrated that all cosolvency models could be made equivalent by using algebraic manipulations. In order to familiarize the readers with the available cosolvency models, they are briefly reviewed. The models can be divided into two mathematical categories, i.e. linear and non-linear models. The linear models include: the log-linear, extended Hildebrand solubility approach, excess free energy equations, combined nearly ideal binary solvent/Redlich-Kister equation and Margule equations which can be converted to a general single model which expresses the logarithm of mole fraction solubility of a solute as a power series of volume fraction of the cosolvent. The non-linear models include the mixture response surface methods, two step solvation model and modified Wilson model which can be converted to a non-linear general form. Also, it has been shown that both the general single model and a non-linear general model are mathematically identical. To show the applicability of the models on real experimental data, 35 data sets have been collected from the literature. Both linear and nonlinear models produced comparable accuracies when an equal number of constant terms was employed in numerical analyses.View full abstractDownload PDF (151K) -
Ichiro Hayakawa, Rieko Shioya, Toshinori Agatsuma, Yuichi Sugano2005 Volume 53 Issue 6 Pages 638-640
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSFor the construction of new combinatorial libraries, a lead compound was created by replacing the core structure of a hit compound discovered by screening for cytotoxic agents against a tumorigenic cell line. The newly designed compound maintained biological activity and allowed alternative library construction for antitumor drugs.View full abstractDownload PDF (114K) -
Tae Hoon Kim, Hideyuki Ito, Kikuyo Hayashi, Toshio Hasegawa, Takahisa ...2005 Volume 53 Issue 6 Pages 641-644
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSA phytochemical investigation of the polar constituents in the heartwood of Indian Santalum album L. resulted in the isolation of three new neolignans (1—3) and a new aromatic ester (4), along with 14 known components. The structures of the new compounds (1—4) were established using spectroscopic methods.View full abstractDownload PDF (163K) -
Yue Wang, Nobuo Okabe2005 Volume 53 Issue 6 Pages 645-652
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSThe crystal structures of the three Zn(II) complexes, [Zn(bpy)(o-AB)2] (1) (bpy=2,2′-bipyridine, o-AB=o-aminobenzoic acid=Vitamin L), [Zn(bpy)(m-AB)Cl]2 (2) (m-AB=m-aminobenzoic acid), [Zn(bpy)(p-AB)Cl]·p-AB·H2O (3) (p-AB=p-aminobenzoic acid=Vitamin H′), have been determined and the basic coordination geometries and architectures organized by hydrogen-bonds and π–π interactions also characterized. The substitute amine group at ortho-, meta-, and para-position of AB plays an important role to produce completely different coordination motif of these complexes, further, in all complexes, aromatic amines are not coordinated to Zn(II) atom. While two different types of coordination modes of the carboxylate O atoms are present in these complexes: one mode consists of the usual Zn–O bond lengths (2.009(2)—2.251(2) Å) in complex 1, 2 and 3; another consists of a very long Zn–O bond lengths (2.422(2) Å) in complex 1. Each of the complexes has the characteristic UV absorption bands around 250—310 nm region, and the intense fluorescence band at near 325 nm.View full abstractDownload PDF (999K) -
Kiyosei Takasu, Tsubasa Shimogama, Chalerm Saiin, Hye-Sook Kim, Yusuke ...2005 Volume 53 Issue 6 Pages 653-661
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSSeveral β-carbolines including naturally occurring substances and their corresponding cationic derivatives were synthesized and evaluated for antimalarial (antiplasmodial) activity in vitro and in vivo. A tetracyclic carbolinium salt was elucidated for antileishmanial and antitrypanosomal activities in vitro as well as antiplasmodial activity. Quarternary carbolinium cations showed much higher potencies in vitro than electronically neutral β-carbolines and a good correlation was observed between π-delocalized lipophilic cationic (DLC) structure and antimalarial efficacy. β-Carbolinium compounds exhibit medium suppressive activity in vivo against rodent malaria.View full abstractDownload PDF (243K)
Notes
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Robert Roškar, Vojko Kmetec2005 Volume 53 Issue 6 Pages 662-665
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSMoisture content of solid-state pharmaceutical products is one of the main factors that affect drug stability, therefore suitable sorption studies need to be performed to assure drug quality throughout their shelf life. The Brunauer–Emmett–Teller (BET) and the Guggenheim–Anderson–de Boer (GAB) models are usually used for this purpose. Using gravimetrically obtained data, both methods were applied in the present work to evaluate the sorption characteristics of several excipients. Microcalorimetric analysis was also performed in order to evaluate the interaction between water and the substances. The results of these experiments show excellent agreement between data and the BET model up to 55% RH and the GAB model over the entire humidity range, confirmed by high values of the statistical determination coefficients. Furthermore, microcalorimetric measurements suggested that the hygroscopicity of solid materials could be estimated approximately using this approach.View full abstractDownload PDF (167K) -
Sadamu Katayama, Masashige Yamauchi2005 Volume 53 Issue 6 Pages 666-670
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSTreatment of 1,8-di-tert-butyldimethylsilyloxybicyclo[2.2.2]oct-5-en-2-ones having an electron-withdrawing group such as a nitro, formyl, cyano, and imido group at C-7 with a strong base (potassium hydride, or potassium bistrimethylsilylamide, etc.), resulted in an acyloin rearrangement reaction accompanied by retention of two silyloxy groups to afford 1,8-disilyloxybicyclo[3.2.1]oct-3-en-2-ones.View full abstractDownload PDF (244K) -
Abdel-Rahim Sayed Ibrahim2005 Volume 53 Issue 6 Pages 671-673
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSBiotransformation of chrysin by Cunninghamella elegans NRRL 1392 produced apigenin, apigenin 7-sulfate, apigenin 7,4′-disulfate, and a new metabolite identified as chrysin 7-sulfate. On the other hand, fermentation of apigenin, using the same microorganism, yielded apigenin 7-sulfate and apigenin 7,4′-disulfate. The structures of the metabolites were established by spectral analysis, and acid and enzyme hydrolyses in addition to comparison with reference samples.View full abstractDownload PDF (67K) -
Shi-Sheng Wang, Wei-Jie Zhao, Xiu-Wen Han, Xin-Miao Liang2005 Volume 53 Issue 6 Pages 674-676
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSTwo new iridoid glycosides designated as senburiside III (2) and senburiside IV (3), together with one known iridoid glycoside senburiside I (1) and three known secoiridoid glucosides swertiamarin (4), gentiopicroside (5) and sweroside (6), were isolated from the whole plant of Swertia franchetiana. The structures of the two new compounds were elucidated by spectroscopic methods.View full abstractDownload PDF (128K) -
Ping Hu, Qiong-Lin Liang, Guo-An Luo, Zhong-Zhen Zhao, Zhi-Hong Jiang2005 Volume 53 Issue 6 Pages 677-683
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSThe multi-component fingerprinting method of Radix Salviae Miltiorrhizae (Root of Salvia miltiorrhiza BGE.), an important and popular medicinal herb in traditional Chinese medicine, was studied using reverse-phase HPLC and LC-MS-MS. Extract containing both the water-soluble phenolic compounds and nonpolar diterpenoid compounds known to be the herb's main bioactive components was prepared by a two-step extractive procedure. An HPLC fingerprinting method which can simultaneously separate these two types of compounds was established with gradient elution mode and photodiode array detection at 280 nm. Eighteen peaks in this HPLC fingerprint were structurally identified by employing LC-MS-MS techniques. The electrospray ionization (ESI) MS-MS spectra of most salvianolic acids displayed a characteristic behavior of loss of danshensu and caffeic acid moieties, while those of tanshinones showed a particular behavior of loss of H2O which is quite different from the fragmentation pattern in electron ionization mass spectrometry (EI-MS). The HPLC fingerprints of 7 batches of crude drugs showed similar separation pattern and provided much chemical information of the pharmacologically-active compounds in the crude drugs, which is useful for the authentication and quality evaluation of this medicinal herb.View full abstractDownload PDF (566K) -
Rika Nakajima, Machiko Ono, Sadakazu Aiso, Hiroyuki Akita2005 Volume 53 Issue 6 Pages 684-687
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSFor the purpose of the direct characterization of the intact conjugated form in the urine of a methamphetamine (MA) abuser, 4-hydroxymethamphetamine (4-OHMA) glucuronate, corresponding to one of the metabolites of MA, was synthesized from the commercially available methyl 4-hydroxyphenylacetate.View full abstractDownload PDF (137K) -
Makoto Ichikawa, Nagatoshi Ide, Sumihiro Shiraishi, Kazuhisa Ono2005 Volume 53 Issue 6 Pages 688-690
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSCombination of cyanocobalamin (VB12) and ascorbic acid (VC) has been widely seen in pharmaceutical products and dietary supplements. However, VB12 has been reported that its behavior in stability in aqueous solution is quite different when VC is mixed. In the present study, we examined the stabilities of these vitamins in acetate buffer (pH 4.8) using high performance liquid chromatography. Degradation of VB12 was not observed in the absence of VC in the buffer. However, when VC was mixed in the VB12 solution, VB12 concentrations decreased in accordance with VC degradation. VB12 and VC degradations were inhibited by adding sodium halides to acetate buffer at pH 4.8. These stabilization effects were also observed in the range from pH 3.5 to 5.3 and by adding potassium, magnesium, and calcium halides. Furthermore, our data demonstrated that increases in the halide anion concentrations and atomic number (Cl−<Br−<I−) were proportionally associated with better stabilities of both VB12 and VC. Therefore, choosing an appropriate condition with a certain halide salt may be useful for stabilizing pharmaceutical products and dietary supplements when VB12 and VC are combined in solution.View full abstractDownload PDF (92K) -
Sukala Kanna Babu, Katta Vijaya Kumar, Gottumukkala Venkata Subbaraju2005 Volume 53 Issue 6 Pages 691-693
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSA simple, sensitive and precise high-performance thin-layer chromatographic (HPTLC) method of analysis of trans-resveratrol in Polygonum cuspidatum root extracts and in dosage forms was developed and validated. The separation was carried out on a TLC aluminium plates precoated with silica gel 60F-254 as the stationary phase, eluted with chloroform–ethylacetate–formic acid (2.5 : 1 : 0.1) as mobile phase. Densitometric analysis of trans-resveratrol was carried out in the absorbance mode at 313 nm. This system was found to give compact spot for trans-resveratrol (Rf value of 0.40±0.03). A good linear regression relationship between peak areas and the concentrations was obtained over the range of 0.5—3.0 μg/spot with correlation coefficient 0.9989. The limit of detection and quantification was found to be 9 and 27 ng/spot. The method was validated for precision and recovery. The spike recoveries were within 99.85 to 100.70%. The RSD values of the precision in the range 0.37—1.84%. The proposed developed HPTLC method can be applied for identification and quantitative determination of trans-resveratrol in herbal extracts and dosage forms.View full abstractDownload PDF (287K) -
Goo Yoon, Young Do Jung, Seung Hoon Cheon2005 Volume 53 Issue 6 Pages 694-695
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSTwo known retrochalcones, licochalcone A (1) and licochalcone C (2), and one new retrochalcone, licochalcone E (4) were isolated by cytotoxicity-guided fractionation from the roots of Glycyrrhiza inflata along with an ordinary chalcone, isoliquiritigenin (3). The structure of the new retrochalcone was elucidated through a spectroscopic analysis.View full abstractDownload PDF (74K) -
Ju Sun Kim, Sang Hee Shim, Sungwook Chae, Sang Jun Han, Sam Sik Kang, ...2005 Volume 53 Issue 6 Pages 696-700
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSA new triterpenoid saponin, together with five known saponins, were isolated from the nonpolar n-hexane fraction of the leaves of Aralia elata. The structure of the new saponin, durupcoside C, was elucidated as hederagenin 3-O-β-D-glucopyranosyl(1→3)-β-D-glucopyranosyl(1→3)-α-L-arabinopyranoside on the basis of spectroscopic analysis. The known saponins were characterized as 3-O-α-L-rhamnopyranosyl(1→2)-α-L-arabinopyranosyl hederagenin 28-O-β-D-xylopyranosyl(1→6)-β-D-glucopyranosyl ester, hederagenin 3-O-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl(1→2)-α-L-arabinopyranoside, oleanolic acid 3-O-β-D-glucopyranosyl(1→3)-α-L-rhamnopyranosyl(1→2)-α-L-arabinopyranoside, hederagenin 3-O-α-L-rhamnopyranosyl(1→2)-α-L-arabinopyranoside (α-hederin), and hederagenin 3-O-β-D-glucopyranosyl(1→3)-α-L-arabinopyranoside (collinsonidin). In addition, two known lipids, Arisaema glyceride 3 and ceramide mixtures were also isolated and characterized. Collinsonidin and two known lipids were isolated for the first time from this plant.View full abstractDownload PDF (99K) -
Masahiko Taniguchi, Osamu Yokota, Makio Shibano, Nian-He Wang, Kimiye ...2005 Volume 53 Issue 6 Pages 701-704
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSFour new coumarins (1—4) were isolated from the roots of Heracleum yunngningense HAND.–MASS. Their structures were established by spectral analyses.View full abstractDownload PDF (157K) -
Ping Hu, Guo-An Luo, Zhong-Zhen Zhao, Zhi-Hong Jiang2005 Volume 53 Issue 6 Pages 705-709
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSAn HPLC-ESI-MS-MS method has been developed for the quantitative determination of four diterpenoids, dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA in Radix Salviae Miltiorrhizae (RSM, the root of Salvia miltiorrhiza BGE.). The diterpenoids were chromatographically separated on a C18 HPLC column, and the quantification of these diterpenoids was based on the fragments of [M+H]+ under collision-activated conditions and in Selected Reaction Monitioring (SRM) mode. The quantitative method was validated, and the mean recovery rates from fortified samples (n=5) of dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA were 95.0%, 97.2%, 93.1%, and 95.9% with variation coefficient of 6.0%, 4.3%, 3.7%, and 4.2%, respectively. The established method was successfully applied to the quality assessment of seven batches of RSM samples collected from different regions of China.View full abstractDownload PDF (600K) -
Suresh Awale, Tsukasa Kawakami, Yasuhiro Tezuka, Jun-ya Ueda, Ken Tana ...2005 Volume 53 Issue 6 Pages 710-713
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSFrom the water extract of Brazilian Tabebuia avellanedae, two new iridoids (1, 2) and a new phenylethanoid glycoside (3) have been isolated together with twelve known compounds (4—15). Their structures were determined based on the spectroscopic data. The isolated compounds inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-activated macrophage-like J774.1 cells. Compounds 1, 3, 10, 11, and 12 showed inhibitory activities more potent (IC50, 13.8—26.1 μg/ml) than a positive control NG-monomethyl-L-arginine (L-NMMA; IC50, 27.4 μg/ml).View full abstractDownload PDF (138K) -
Masahiro Nishizawa, Masahiro Kohno, Minemitsu Nishimura, Akio Kitagawa ...2005 Volume 53 Issue 6 Pages 714-716
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSA stable radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) has long been used as a convenient method for the antioxidant assay of biological materials such as cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxy aromatic compounds (hydroquinone, pyrogallol, etc.). In this study, non-reductive scavenging of DPPH was investigated by electron spin resonance (ESR) analyses for the purpose of developing a useful method for quantitative determination of peroxyradical. Since DPPH was degraded in the presence of peroxyradical derived from UV-irradiated benzoylperoxide and the peroxyradical-induced degradation of DPPH was inhibited by the addition of a spin trapping agent 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), it is concluded that DPPH is non-reductively scavenged by peroxyradical. Therefore, it is suggested that DPPH could be a useful agent for the quantitative measurement of peroxyradical.View full abstractDownload PDF (142K) -
Takeshi Kinoshita, Yasuhiro Haga, Shintaro Narimatsu, Motoko Shimada, ...2005 Volume 53 Issue 6 Pages 717-720
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSNew cassane diterpene-acids, neocaesalpins H and I, were isolated from the leaves of Caesalpinia crista (Fabaceae), and their structures were deduced on the basis of the spectroscopic and chemical basis. These compounds were characterized as having an α,β-butenolide hemiacetal ring that is rare in nature. The lacking of 5-hydroxy group also distinguished neocaesalpins H and I from cassane diterpenes (caesalpins) occurring in other Caesalpinia species from the phytochemical viewpoint. The nomenclature of three Caesalpinia species was also reviewed, and it was found that some species belonging to the genus Caesalpinia are improperly named and should be changed to valid names.View full abstractDownload PDF (180K)
Errata
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2005 Volume 53 Issue 6 Pages 721
Published: 2005
Released on J-STAGE: June 01, 2005
JOURNAL FREE ACCESSDownload PDF (17K)
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