1.) Transfer of
durum genomes into
ovata cytoplasm of
Aegilotricum No. 2 was made in order to ascertain if male-sterility might always occur even if any lines of
Aegilotrium No. 2 are used. All resulting offspring be-came completely male-sterile as in previous experiments.
2) Using pure
ovata plants as a female parent,
durum genomes were substituted into
ovata cytoplasm. Resulting 14
II-plants become male-sterile without exception.
3) Using the new synthesized amphidiploid (
durum_??_+
ovata _??_), restoration of
durum genomes was made. All 14
II-offspring showed normal pollenf ertility.
4)
Ovata genomes were replaced again in the cytoplasm of male-sterile
durum plants. All resulting 14
II-individuals were normal with high pollenfertility.
5) Placing the
ovata genomes into
durum cytoplasm was carried out. Most of 14
II-plants showed normal pollen-fertility.
6) It has been discussed that the male-sterile factor came from the
ovata cytoplasm and that
ovata plants contain a certain nuclear gene which makes the male-sterile factor inactive.
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