The sample of the human intestinal parasitic flagellate,
Giardia lamblia P
1 (P
1 Tr
1) and the supernatant fluid (Su Tr
2) and the precipitate suspended in saline (SSP Tr
3) of the centrifuged autoclaved
G. lamblia culture were intraperitoneally injected individually to separate sets of mice at the rate of 1ml per 100g body weight against sterile TYI medium (TYI Co
1) and nomal saline (Sa Co
2) injected controls which yielded maximum cytogenetic effects with respect to bone marrow chromosome aberration frequency, micronucleated erythrocytes and sperm head abnormality tests in supernatant fluid treated sample (Su Tr
2) and followed in descending order by the treatment of
G. lamblia P
1 sample (P
1 Tr
1) and saline suspended precipitate sample (SSP Tr
3). At 24 hr after treatment the net increase in frequency of effect in three different samples of
G. lamblia P
1 treated series over respective controls was 17.75%, 8.50% and 5.75% respectively for bone marrow chromosome aberration; 0.83%, 0.61% and 0.40% respectively for MNT and 3.15%, 2.00% and 1.10% respectively for sperm head abnormality. Further, the frequency of bone marrow chromsome aberration for the treatment of
G. lamblia P
1 sample (P
1 Tr
1) was also found higher by 2.5% at 3 hr and 12.5% on 7 days over control. On 7 days after treatment it was also higher by 0.83% for MNT and 2.85% for sperm head abnormality. Thus the mutagenic potential of different samples of
G. lamblia P
1 in treated mice was proved by more than one test adding a new evidence to the field of living mutagenesis.
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