CYTOLOGIA
Online ISSN : 1348-7019
Print ISSN : 0011-4545
68 巻 , 4 号
選択された号の論文の18件中1~18を表示しています
  • V. M. Saitwal, S. S. Mehetre, A. H. Sonone, V. L. Gawande
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 317-327
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    A detailed comparative cytological status of parents, F1, F2 and BC1 have been made. The cytological studies showed that F1, F2 and BC1 generations were having irregular pairing and unequal separation of chromosomes. This led to pollen sterility in these generations. However, in BC1 bivalents frequency and normal tetrad formation found to be improved. The morphological studies revealed that interspecific hybrid was partial fertile and vigorous. In F2 and BC1 some good plants are identified with higher pollen fertility and more number of bolls per plant. Selection from these plants can help to introgress desired characters from G. anomalum to G. arboreum.
  • Herbán Cequea, Damelis Díaz de Cequea, José Imery, Maur ...
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 329-333
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    Meiotic analyses at pachytene, anaphase I and II, metaphase II and the tetrad stage were carried out on several Tridax procumbens L. plants. Aberrations such as reverse inversion loops in pachytene, dicentric bridges and (or) fragments in pollen mother cells at anaphase I and II, dicentric bridges at metaphase II and 4–7 microspores at the tetrad stage, indicated that these plants were heterozygous for a paracentric inversion, which may involve one or more homologous chromosomes. The 75% fertility showed that a simple or double crossing-over took place within inversion reverse loops between inverted and normal chromosome segments at pachytene, which reduced fertility by the formation of genetically abnormal gametes.
  • Zheng-Song Peng, Jun Yang, Guo-Chang Zheng,
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 335-340
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    Hexaploid amphidiploid was obtained from the cross of a tetraploid wheat (T. turgidum L.) landrace Aiganfanmai with Aegilops tauschii, and cytological investigation was carried out during microsporogenesis of this material. High frequency of cytomixis was observed in the pollen mother cells of the new synthetic hexaploid amphidiploid, which occurred at synizesis stage of meiosis. Most chromatins migrated along the pollen sac, only a few were observed to move in cross direction. The chromatins were noticed to transfer from one to one or more adjacent pollen mother cells. Furthermore, aberrant chromosome numbers were found in the new synthetic hexaploid amphidiploid, which suggested that cytomixis might be one cause of the instability of new synthetic amphidiploid.
  • Kellen Regina Boldrini, Nilton Cesar Pires Bione, Maria Suely Pagliari ...
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 341-344
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    Codiaeum variegatum shows wide spectrum variability in chromosome numbers among cultivars and plant cells. Chromosome instability in somatic cells has been attributed to endoreduplication, irregular chromosome segregation, spindle multipolarity and unusual nucleus shape. Cytological studies of microsporogenesis in garden crotons revealed that chromosome transfer between microsporocytes in early meiosis might also be included in the row of abnormalities responsible for variations in chromosome number. Six in 12 plants analyzed presented cytomixis among microsporocytes in frequencies ranging from 0.30 to 10.45%. Transference of few chromosomes to the whole genome was found among 2 cells. Prior to cytomixis, chromatin underwent structural alteration. Plants were male-sterile because cytokinesis did not occur after telophase II and tetrad formation was replaced by four-nucleate coenocytic microspore that underwent degeneration prior to callose dissolution.
  • Lenice de Souza, Lucia Giuliano-Caetano, Ana Lúcia Dias
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 345-350
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    The present study involved the karyotypic analysis of Pimelodus argenteus, P. mysteriosus and P. maculatus, from the the Paraguai River, in the municipality of Corumbá-MS/Brazil. All the species had a diploid number of 56 chromosomes with differences in the karyotypic formulae. In P. argenteus, there was a secondary constriction, quite conspicuous in one of the homologous chromosomes. NOR staining was observed on one pair of ST chromosomes at the terminal position in the three species, in which it was localized on short arm in P. argenteus and P. mysteriosus and on long arm in P. maculatus. In P. argenteus and P. mysteriosus, C-banding showed heterochromatin distributed homogeneously in various chromosomes of the complement, mainly in telomeric regions. In P. maculatus, heterochromatic staining was found to be very evident in one pair chromosomes with strong interstitial staining. The fluorochrome CMA3 stained in P. argenteus and P. mysteriosus only the NOR-bearing pair of chromosomes. In P. maculatus, fluorescent staining was observed in various chromosomes in addition to the NOR pair, including one pair with interstitial staining. Treatment with BC+CMA3 produced in P. argenteus and P. mysteriosus a much more fluorescent staining of the pair of NOR-bearing chromosomes, mainly in P. argenteus, along with weak staining of other chromosomes. In P. maculatus, fluorescent staining was much brighter after this treatment.
  • Margarete Magalhães de Souza, Ernane Ronie Martins, Telma Nair Sa ...
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 351-356
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    Reproductive studies were carried out on Brazilian accessions of Cephaelis ipecacuanha, which is considered a threatened species. Meiotic behavior was studied using squashing technique with 1% acetic carmine and floral buds and anther were measured for the following male gamete development stages: meiosis I, meiosis II, tetrads, microspores and pollen. Bud and anther length showed significant differences (P<0.01) among the stages of pollen development. There was high significant correlation between bud and anther length by Pearson correlation. Tukey test results indicated that each analyzed stage could be associated to bud length mean but it was not found the same association for anther length.
  • Sangram Sinha, Anupam Guha, Rabindra Kumar Sinha
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 357-361
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    Cytological and immunochemical studies were carried out on dioecious Trichosanthes dioica to understand the genetic control of sex expression. The karyotype shows high homogenity and the absence of heteromorphic pair of chromosome in diploid males negates the possibility of XY mechanism. Soluble protein profile from the tuberous roots of the male and female plants of T. dioica did not show any marked distinction; only a variation in the staining pattern was observed. However, in the immunoblot assay the cross reactivity of anti-p-22 (antibody raised against a female biased 22 kD protein of Momordica dioica) at 29 kD and 32 kD regions of the protein profiles in both sex forms establishes p-22 as a sex linked marker. The ratio of the intensity of 29/32 kD polypeptide is also an indicator of sex expression and the data has been analysed as well.
  • Alberto S. Fenocchio, Luiz A. C. Bertollo, Catarina S. Takahashi, Ana ...
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 363-368
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    In Pimelodidae fish the available chromosome numbers range from 2n=46 to 2n=63, the value 2n=56 being the most frequent one. In the present paper 6 species belonging to the Rhamdiinae subfamily were analyzed: Rhamdia hilarii, R. quelen, Cetopsorhamdia sp., C. iheringhi, Imparfinis cf. piperatus and Imparfinis aff. schubarti. Chromosome preparations were obtained by direct and short term culture methods from kidney cells and analysed under standard Giemsa staining, C-banding and NOR silver staining. Rhamdia species present a basic karyotype composed by 58 chromosomes and the NORs are located on the short arm of a subtelocentric chromosome pair. Cetopsorhamdia sp., C. iheringhi and Imparfinis aff. schubarti. also shows 2n=58, while I. cf. piperatus is the only analyzed species presenting 2n=56. However, these 4 species show an interstitial NOR location. These chromosomal data agree with the occurrence of subgroups in the Rhamdiinae subfamily, proposed on the basis of their morphological traits.
  • Tinnanooru Pushpalatha, Pamuru Ramachandra Reddy, Geddapara Trivikram, ...
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 369-373
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    Prenatal exposure to hydroxyprogesterone can alter the spermatogenesis in adult rat. For the purpose of assessing the effects of prenatal exposure to hydroxyprogesterone on postnatal reproductive performance, rats were treated intraperitoneally with doses of hydroxyprogesterone (10, 25 mg/kg body weight) on 1st, 7th and 14th day of gestation. The reproductive performance of the male offspring was assessed after 90 d by determining the changes in testis weight and the number of spermatids. A significant (p<0.001) decrease in testicular weight and number of spermatids was observed in the experimental male rats. Testis of the experimental rats also exhibited symptoms of arrest of spermatogenesis. It is suggested that in utero exposure to supra-normal levels of hydroxyprogesterone affects male reproduction by altering the spermatogenesis.
  • Illairaja Ismail Mujawar, Sayajirao Pandurang Gaikwad, Srirang Ramchan ...
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 375-378
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    Somatic chromosome number (2n) and karyomorphology have been reported for first time in Wiesneria triandra (Dalz.) Micheli, a member of most archaic family Alismataceae. In general, the chromosomes are long with median, submedian and subterminal primary centromeres. The karyotype is reasonably asymmetrical and indicated advances in general. 10 distinct bivalents at diakinesis were observed during meiosis.
  • Puangpaka Soontornchainaksaeng, Pranom Chantaranothai, Chadaporn Senak ...
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 379-382
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    Genetic diversity of Croton 15 species were investigated. The results revealed that chromosome numbers of Croton are quite unique of 2n=20, except C. hirtus which contains 2n=16. Chromosome numbers of 9 species are the first recorded. The genus Croton has base number of 8 and 10. Chromosomes of microsporocytes of Croton are very well stained with propionocarmine. Most of them are clearly paired in bivalent of 1.00–6.00 μm at metaphase I cells of 26.67–63.33 μm (Ø). Chromosomes are balanced disjunction at anaphase I. The results show that Croton are very good fertile and stable in natural habitat and it has a small genome which could be manipulated or improved simply. C. hirtus is probably more advanced than the others.
  • Animesh Kumar Datta, Anindita Ghosh, Sonali Sengupta
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 383-388
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    Two aberrant (plant type I and II) plants possessing meiotic instability in the microsporocytes were identified from the mutant population of Nigella damascena L. (commonly known as love-in-a-mist; family: Ranunculaceae). Plant type I (screened from 10 kR M1 generation) was a weak plant with small sized flowers and shrunken anthers devoid of any pollen grains at anthesis. The plant dried up at blooming stage. Male meiotic study revealed the occurrence of condensed and compact chromatin masses of variable sizes and numbers (12 to 13 mostly and 3 to 5 rarely) and occasional formation of bivalent and univalent like configurations in the meiocytes. Plant type II (isolated at M2 from the M1 progeny of 0.25% EMS treatment) was a small flower mutant (SF2), the morphological nature and chromosomal behaviour of which have been compared to control and SF1 (small flower mutant arising in different M2 line). SF1 showed similar morphological behaviour as SF2 but demonstrated normal meiosis like control. SF2 manifested hypoploid (2n<12), diploid (2n=12) and hyperploid (2n>12) chromosome numbers at MI and AI and prevalence of unstable meiosis in the form of asynaptic and desynaptic chromosome behaviour, differential condensation of chromosomes, persistent nucleolar body and occasional multivalent formation in first and mid-flowering phases. SF2 showed more diploid PMCs at late flowering phase. SF2 was also found to be associated with defects in female gametophytic tissues. Possible mode of gene action in the plant types has been predicted.
  • Lana Cristina Fazoli, Valquíria de Almeida Bernardo da Silva, Ana ...
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 389-394
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    Cytogenetics studies on Astyanax sp B, an endemic species of the Iguaçu River (Paraná, Brazil) were carried out. The specimens showed a standard diploid number of 2n=50 chromosomes with a karyotype consisting of 6 metacentric, 24 submetacentric, 6 subtelocentric and 14 acrocentric chromosomes. In addition to the basic karyotype, all the specimens presented 1–2 supranumerary or B chromosomes in mitotic metaphases. Three morphological types of B chromosomes have been identified in population under analysis: an acrocentric-type microchromosome; a metacentric macrochromosome smaller than the first pair of the normal karyotype complement; a small submetacentric chromosome. C-band analysis shows discrete markings in the centromeric regions of various chromosomes which contrast with strongly heterochromatic blocks in the telomeric regions of the long arms of chromosome pairs 20, 21 and 22 (acrocentric). Heterochromatic block-sized heteromorphism has been among homologues of pair 20. The chromosome characterisitics of Astyanax sp B indicate similarity with other species of the genus Astyanax with regard to karyotypic macrostructure, distribution of constitutive heterochromatin (C-band pattern) and occurrence of B chromosomes. Some aspects related B chromosomes in this species are discussed.
  • Silvia Tamie Matsumoto, Mário Sérgio Mantovani, Mirtis I. Ma ...
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 395-401
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    The comet assay has been described as an efficient tool for the detection of changes in the DNA molecule of cells exposed to contaminating agents in vivo and in vitro. The possible environmental contamination due to the persistence of chromium residues from tannery effluents was determined in the waters of the “Córrego dos Bagres” stream, Municipal district of Franca/SP, by the comet assay on CHO-K1 cells. Water samples were collected during the four seasons of the year 2001 at three distinct stations along the river. The data suggest that the comet test showed good sensitivity for the environmental monitoring of these waters and indicated that this test can be efficient for the determination of the quality of waters contaminated with effluents containing heavy metal residues such as chromium.
  • Caroline Garcia, Orlando Moreira-Filho, Luis Antônio Carlos Berto ...
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 403-411
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    Cytogenetical studies were carried out on 13 specimens of Rhamdia sp. from Grande stream (Paraíba do Sul river basin, Mantiqueira hills, Brazil). It was evidenced a standard diploid number 2n=58 chromosomes (46M/SM and 12ST), plus 1 to 4 B chromosomes, found in 10 specimens. One individual presented natural triploidy, lacking B chromosomes. Fluorescent in situ hybridization with 5S and 18S rDNA probes was employed to improve the conventional karyotypical analyses. Inferences about the presence of supernumerary chromosomes within the genus Rhamdia, based on the karyotypical evolutionary pattern of this group, as well as the reoccurrence of natural triploidy in fish at Mantiqueira hills are discussed.
  • Hidehiro Hanmoto, Kazuo Fujikawa, , Tetsuo Itoh, Yoshihiko Yonezawa
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 413-424
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    Following exposure to X-rays or reactor radiation at the early germinating stage, seedlings of Haplopappus gracilis (Nutt.) Gray were inspected for karyotypic changes in the meristems of lateral roots 2 months later. Among 210 plants studied in total, 12 were carriers of aberrant karyotypes in the root systems. One of them was a whole-body variant of spontaneous origin, showing a complete change of karyotype not only in the root system but also in the shoot system. The remainders were mosaics, showing a complete karyotypic change in one or more lateral roots but not in the whole root system. Of the 11 cases of aberrant karyotypes detected as inter-root mosaics, 5 were unique to the respective plants, each characterized by a specific kind of non-reciprocal translocation, reciprocal translocation or complex exchange; 2 were commonly characterized by a complex exchange; 3 were by a non-reciprocal translocation; one was characterized by a fragment chromosome accompanied with a shortened chromosome and was not distinguishable from the aberrant karyotype of spontaneous origin. These results support the conclusion that the chromosomes of H. gracilis possess the plasticity to rearrangements after irradiation. The repetitious occurrence of specific rearrangements in different plants and the recovery of fragment-bearing karyotype are discussed as suggestive evidence for the existence of preferential sites for breakage and rearrangement in H. gracilis genome.
  • Ricardo A. Lombello, Cecília A. F. Pinto-Maglio
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 425-429
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    The genus Psilanthus is taxonomically close related to Coffea and its cytogenetic presents few studies due to the symmetry of its small chromosomes. In this work we analyzed cytogenetic features of Psilanthus ebracteolatus, a wild diploid coffee native from Ivory Coast included in the Coffee Germplasm Bank of the Instituto Agronômico de Campinas, Brazil. For heterochromatic banding assays the fluorochromes CMA3 and DAPI were used. For FISH assays the rDNA probes pTa71 and pScT7 were used. This species presented no DAPI positive bands, two CMA3 positive band pairs and one pair of NOR and one of 5S rDNA sites. Although we observed cytological similarity between the studied species and the few data presented for Psilanthus in literature, the analysis based on cytogenetic markers contributes to karyological characterization of this genus.
  • Yumiko Suto, , Takafumi Ishida, Momoki Hirai
    原稿種別: regular article
    専門分野: [not specified]
    2003 年 68 巻 4 号 p. 431-436
    発行日: 2003年
    公開日: 2003/12/30
    ジャーナル オープンアクセス
    By multiplex fluorescence in situ hybridization (M-FISH) that enables the simultaneous identification of 24 human chromosomes, we studied chromosomal homologies between humans (Homo sapiens, 2n=46) and 6 species of Old World monkey, namely, the vervet monkey (Cercopithecus aethiops, 2n=60), patas monkey (Erythrocebus patas, 2n=54), hanuman langur (Semnopithecus entellus, 2n=44), cynomolgus monkey (Macaca fascicularis, 2n=42), stump-tailed macaque (Macaca arctoides, 2n=42) and hamadryas baboon (Papio hamadryas, 2n=42). Individual human chromosomes were found to correspond to either one or two chromosomes of each monkey species without any detectable complex translocation involving more than 2 chromosomes. Based on chromosomal correspondences among these species, we propose a hypothetical karyotype phylogeny.
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