巻号一覧
74 巻 (2009)
- 4 号 p. 367-
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- 2 号 p. 115-
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74 巻, 1 号
選択された号の論文の12件中1～12を表示しています
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Alongkoad Tanomtong, Puntivar Kaewmad, Sumpars Khunsook, Sarawut Kaews ...2009 年 74 巻 1 号 p. 3-15
発行日: 2009/03/30
公開日: 2009/06/10
ジャーナル オープンアクセスThe fishing cat, Prionailurus viverrinus (Bennett 1833) and the Asiatic golden cat, Catopuma temminckii (Vigos and Horsfield 1827) are two of many wild cat species currently listed as vulnerable or endangered in Thailand. 20 conventional staining, G-banding and high-resolution metaphase spreads obtained from lymphocyte cultures were analyzed for each animal. The female fishing cat has diploid chromosome number of 2n=38 and the fundamental number (NF) was 74. There are 6 autosome types: A type had 6 large submetacentric, B type had 8 large acrocentric, C type had 4 large metacentric, D type had 4 medium and 4 small submetacentric, E type had 8 small metacentric and F type had 2 small telocentric chromosomes. The female Asiatic golden cat had 2n=38, and the NF was 72. There are 6 autosome types: A type had 4 large and 2 medium submetacentric, B type had 6 large and 2 medium acrocentric, C type had 4 large metacentric, D type had 8 small submetacentric, E type had 6 small metacentric and F type had 4 small telocentric chromosomes. The female fishing cat and the Asiatic golden cat have a pair of the short arm of chromosome E1 (chromosome pairs 14) showed clearly observable satellite chromosomes. The X chromosome was submetacentric chromosome. From G-banding and high-resolution technique, number of bands were 184, 211 and 204, 245, respectively. We found that chromosomes A1, A2, A3, B1, B2, B4, X (fishing cat) and A1, A2, A3, C2, D1, D4, E1, E2, E3, F1, F2, X (Asiatic golden cat) patterns are same as domestic cat (Felis catus) chromosomes. Chromosomes B3, C1, C2, D1, D2, D3, D4, E1, E2, E3, F1 (fishing cat) and C1, D2, D4 (Asiatic golden cat) are similar to those of domestic cat chromosomes. These results show the evolutionary relationship between the wild cat (fishing cat, Asiatic golden cat) and the domestic cat. The karyotype formula of the female fishing cat and the Asiatic golden cat are as follows:
Fishing cat: 2n(38)=L4m+L6sm+L8a+M4sm+S8m+S4sm+S2t+sex chromosomes.
Asiatic golden cat: 2n(38)=L4m+L4sm+L6a+M2sm+M2a+S6m+S8sm+S4t+sex chromosomes.抄録全体を表示PDF形式でダウンロード (1921K) -
Soamret Sikhruadong, Alongkoad Tanomtong, Weeranuch Wonkaonoi, Bhuvado ...2009 年 74 巻 1 号 p. 17-22
発行日: 2009/03/30
公開日: 2009/06/10
ジャーナル オープンアクセスThe chromosome staining of crinum lily (Crinum asiaticum L.) using 12 natural dyes indicate that white mulberry fruit (Morus alba L.) and black glutinous rice (Oryza sativa L.) can capable for plant chromosome staining and also define mitotic cell division. Seven natural dyes, butterfly pea sepal (Clitorea ternatea L.), Roselle petal (Hibiscus sabdariffa L.), curcuma rhizome (Curcuma longa L.), Malabar nightshade fruit (Basella alba L.), Lac (Laccifer lacca Kerr.), Chan daeng wood (Draceana loureiri Gagnep.) and Fang stem (Caesalpinia sappan L.), show not clearly plant chromosome staining and unable to define the cell division. Three natural dyes, Jackfruit wood (Artocarpus heterophyllus L.), Kumsaed peel (Bixa orellana L.) and Burmese rosewood bark (Plerocapus indicus Willd.), cannot stain the plant chromosomes.抄録全体を表示PDF形式でダウンロード (3881K) -
Alongkoad Tanomtong, Praween Supanuam, Sumpars Khunsook, Chalao Sumran ...2009 年 74 巻 1 号 p. 23-29
発行日: 2009/03/30
公開日: 2009/06/10
ジャーナル オープンアクセスKaryological of the white-cheeked gibbon (Nomascus leucogenys) Nakhonratchasima Zoo, Thailand was studied. Blood samples were taken from 1 female and 2 males. After standard whole blood lymphocyte culture at 37°C for 72 h in the presence of colchicine, the metaphase spreads were performed on microscopic slides and air-dried. The G-banding and high-resolution technique were applied to stain the chromosomes. The results showed that the number of diploid chromosomes of white-cheeked gibbon was 2n (diploid)=52. The type of autosomes were 32 metacentric, 12 submetacentric and 6 acrocentric chromosomes, with the X and Y-chromosome being acrocentric and smallest submetacentric chromosome, respectively. From the G-banding and high-resolution technique, the numbers of bands and locations in the white-cheeked gibbon were 361 and 435 respectively, each chromosome pair could be clearly differentiated. The developing of specific cytogenetics of a species is possible further benefic for humanity.抄録全体を表示PDF形式でダウンロード (733K) -
Masoud Sheidai, Sodabeh Jafari, Parisa Taleban, Maryam Keshavarzi2009 年 74 巻 1 号 p. 31-41
発行日: 2009/03/30
公開日: 2009/06/10
ジャーナル オープンアクセスMeiotic studies were performed on the populations of four Alepecurus L. species and varieties as well as five populations of two Catabrosa species. Alopecurus textilis showed 2n=8x=56 chromosome number, the varieties of A. myosuroides var. myosuroides and A. myosuroides var. breviaristatus showed 2n=2x=14 and A. arundinaceus possessed 2n=6x=42. Catabrosa aquatica and C. capusii showed 2n=4x=20 chromosome number. The chromosome numbers of A. textilis, A. myosuroides var. myosuroides and A. myosuroides var. breviaristatus and C. capusii are new to science, while hexaploid level is new for A. arundinaceus. Tetraploid and octaploid species of Alopecurus and Catabrosa studied formed bivalents in metaphase of meiosis-I, which is considered a diplontic behavior for polyploid species. Grouping of the species by clustering methods based on relative cytogenetical characteristics showed good separation between Alopecurus and Catabrosa species indicating the cytogenetic distinctness of these two grass genera. Cytomixis and chromosome migration occurred in most of the species and populations studied forming aneuploid and polyploid meiocytes. Unreduced pollen grains were formed possibly as a result of cytomixis in the species studied.抄録全体を表示PDF形式でダウンロード (1691K) -
Masoud Sheidai, Marjan Sotoode, Zahra Nourmohammadi2009 年 74 巻 1 号 p. 43-53
発行日: 2009/03/30
公開日: 2009/06/10
ジャーナル オープンアクセスCytogenetic studies concerning karyotype and meiotic analyses were performed in thirty-seven cultivars of C. tinctorius grown in Iran. All the cultivars showed 2n=2x=24 chromosome number having meta and sub-meta centric chromosomes ranging in size from 1.55 to 4.63 μm. The cultivars studied differed significantly in the size of chromosomes and in karyotype formulae indicating the occurrence of quantitative and qualitative changes in their chromosomes. Meiotic analysis showed the occurrence of post pachytene diffuse stage, mostly bivalent formation in metaphase and also the occurrence of quadrivalents due to translocations in some of the cultivars studied. Meiotic abnormalities like chromosomes stickiness, laggard formation, multipolar cells and cytomixis occurred in some of the cultivars leading to pollen sterility and polyploid pollen formation. Grouping of the cultivars based on cytogenetic data showed their genetic differences.抄録全体を表示PDF形式でダウンロード (1076K) -
Hamid Reza Naseri, Hossein Azarnivand, Mohammad Jafari2009 年 74 巻 1 号 p. 55-64
発行日: 2009/03/30
公開日: 2009/06/10
ジャーナル オープンアクセスA karyological study of seven taxa (four sections) of genus Artemisia L. from Azerbaijan region (Iran) is presented. We found the two usual basic numbers in the genus x=9, the most common one (in three diploid and two tetraploid populations) and x=8 (in two diploid populations).The intra- (A1) and interchromosomal (A2) asymmetry index, which does not depend on chromosome number or chromosome size showed asymmetry index based on A1 value has indicated that the chromosomes in some Iranian Artemisia evolved in order of section Absinthium and Artemisia meanwhile section Seriphidium and Dracunculus are close to each other but they can separate from Section Absinthium and Artemisia.抄録全体を表示PDF形式でダウンロード (1147K) -
Emi Hattori, Mitsuo Kawano, Noboru Izuma, Masato Tsurudome, Joji Morit ...2009 年 74 巻 1 号 p. 65-70
発行日: 2009/03/30
公開日: 2009/06/10
ジャーナル オープンアクセスTransgenic (Tg) mice, which carry human CD98 heavy chain (hCD98HC) gene, were made for the first time. Biochemical analyses of total protein, albumin, cholesterol, glutamic-oxaloacetic transaminase (GOT), glutamic-pyruvic transaminase (GPT) and alkaline phosphatase (ALP) in the sera were carried out. The expression of hCD98HC and murine CD98HC (mCD98HC) mRNA, and the tissue distribution of hCD98HC and mCD98HC were determined.
The values of total protein, albumin, cholesterol, GOT, GPT and ALP of Tg mice were about the same as those of normal mice. The mCD98HC mRNA was highly expressed in all the tissues of normal mice examined except for skeletal muscle, where it was expressed rather slightly. hCD98HC mRNA of Tg mice was detected on cDNA prepared using oligo-dT primer, and the mRNA was clearly found in brain, kidney, skin, spleen, stomach, testis and thymus. However the mRNA was rather slight in liver, skeletal muscle and intestine, and almost nothing in heart. Immunofluorescence study of murine tissue sections using monoclonal antibody (mAb) directed against mCD98HC revealed that mCD98HC was clearly expressed in renal proximal tubules of kidney, follicular epitherium and keratinocyte of skin, and interstitial space including Leydig cell of testis. However, hCD98HC in Tg mice, determined by immunostaining using hCD98HC mAb, was not detected in any of the tissues examined.抄録全体を表示PDF形式でダウンロード (1507K) -
Valiya Purakkal Sobhakumari, Sara Deepa Mathew2009 年 74 巻 1 号 p. 71-77
発行日: 2009/03/30
公開日: 2009/06/10
ジャーナル オープンアクセスThe influence of hybrid vigor on callus induction, culture efficiency, callus weight, regeneration capacity of the callus and number of regenerated plants from callus culture of sugarcane was studied. For the study a total of 6 parents (clones of S. officinarum and S. spontaneum) and 12 F1 hybrids were used. The statistical analysis of the results revealed that positive heterosis was noticed for callus induction, callus weight and number of regenerated plants.抄録全体を表示PDF形式でダウンロード (2573K) -
Alireza Doulatyari, Seyed Mahmood Ghaffari, Hossein Akhani2009 年 74 巻 1 号 p. 79-87
発行日: 2009/03/30
公開日: 2009/06/10
ジャーナル オープンアクセスOriginal chromosome numbers and meiotic behavior are presented for 25 populations belonging to 14 halophytic species of the genera Climacoptera Botsch., Halimocnemis C. A. Mey. s.l., Petrosimonia Bunge, Halocharis Moq. and Kaviria Akhani & E. H. Roalson (Caroxyloneae) and Salsola florida (M. Bieb.) Poir (Salsoleae). The basic chromosome number of x=8 was found in Petrosimonia and x=9 in other genera. The polyploidy was detected in Kaviria tomentosa (Moq.) Akhani (n=18), Climacoptera crassa (M. Bieb.) Botsch. (n=27) and C. turcomanica (Litw.) Botsch. (n=18, 27). Chromosome numbers of the following species are reported for the first time: Halimocnemis azarbaijanensis Assadi (n=9), H. gamocarpa Moq. (n=9), H. pilosa (Pall.) Akhani (n=9), H. mamamensis (Bunge) Assadi, H. rarifolia (C. Koch) Akhani (n=9), H. mollissima Bunge (n=9), H. pilifera Moq. (n=9), Halocharis sulphurea (Moq.) Moq. (n=9), Petrosimonia glauca (Pall.) Bunge (n=8), and Salsola florida (M. Bieb.) Poir (n=9). New ploidy levels are also reported for the first time in Climacoptera crassa and C. turcomanica. Meiotic behavior was generally regular in all cases but some irregularities such as laggard chromosomes in Metaphase II were observed in Halimocnemis pilifera. Our results together with available literature data suggest that x=9 is the basic chromosome number for the Chenopodiaceae family and the other numbers (e.g. x=8 as in Petrosimonia glauca) are derived from dysploidy series. The lowest frequency of chiasma is reported for Halimocnemis mamamensis (1.1 per each bivalent) and the highest for Kaviria tomentosa (1.6 per each bivalent).抄録全体を表示PDF形式でダウンロード (5309K) -
Ritambhara Shukla nee Tripathi, Girjesh Kumar2009 年 74 巻 1 号 p. 89-93
発行日: 2009/03/30
公開日: 2009/06/10
ジャーナル オープンアクセスDuring the meiotic analysis of gamma ray treated sets, two induced translocation heterozygotes (P-1 and P-8) were cytologically isolated at 200 Gy and 400 Gy doses in Lathyrus sativus L. var. Pusa-24. However, both translocation heterozygotes (P-1 and P-8) exhibited the formation of a ring and chain of multivalents in majority of PMCs at diakinesis and metaphase-I. At anaphase-I, both the translocation heterozygotes displayed various abnormalities as well, although some PMCs showed normal 7 : 7 separations but the most common and frequent separation to be encountered in these two variants was 6 : 8 separation. Pollen fertility was found to decline to 41.32% in P-1 and 39.46% in P-8 against 98.92% in the control sets.抄録全体を表示PDF形式でダウンロード (2425K) -
Junji Moribe, Song Li, Yingxiang Wang, Shuji Kobayashi, Sen-ichi Oda2009 年 74 巻 1 号 p. 95-99
発行日: 2009/03/30
公開日: 2009/06/10
ジャーナル オープンアクセスKaryological data on the lesser stripe-backed shrew, Sorex bedfordiae, were obtained from four specimens collected on Mt. Laojun, Lijiang District, Yunnan Province, China. Three of the four S. bedfordiae specimens had karyotypes consisting of 2n=26, NFa=44, with 4 metacentric, 4 submetacentric, 2 subtelocentric, and 2 acrocentric pairs, and 1 acrocentric X and acrocentric Y. This karyotype has the smallest diploid chromosome number in the XY group in the genus Sorex. The fourth specimen of S. bedfordiae had a different karyotype consisting of an additional pair of medium-sized metacentric chromosomes, i.e., 2n=28, NFa=48. These supernumerary chromosomes appear to constitute a new chromosome group in the genus Sorex and might provide new information concerning the taxonomy of soricid species.抄録全体を表示PDF形式でダウンロード (196K) -
Salwa Badr, Abd El-zahar Mustafa, Walla Taher, Reda Helmy Sammour2009 年 74 巻 1 号 p. 101-111
発行日: 2009/03/30
公開日: 2009/06/10
ジャーナル オープンアクセスThe genetic variability based on karyotype formula was studied for 40 accessions of genus Lathyrus. All accessions have 2n=14 chromosomes. Karyotype formula has a great uniformity among accessions of each species, suggesting interaspecific stability in Lathyrus species. Our data allowed the differentiation of several accessions among sections Lathyrus, Linearicarpus and Clymenum in number of m chromosome. The variation among section Lathyrus supports that section Lathyrus is not fully constant as has been postulated. Satellites were detected in a pair of chromosome in L. sativus (TUN and AFG). On the otherhand, B-chromosome was detected in L. sativus (USSR, BAN, CAN, and PAK) L. gorgoni (JOR) and L. annus (SYR). This finding may be due to the variation in DNA amount by increase in the non-repetitive sequence. The variation in size of chromosomes of the members of Lathyrus is associated with the evolution in the genus.抄録全体を表示PDF形式でダウンロード (2903K)
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