The division of the generative cell of Impatiens Balsamina was studied in pollen-tube cultures.
The generative cell enters the tube in prophase and remains in this stage for about 30 minutes after germination. Metaphase lasts about 5-10 minutes; 40 minutes after germination anaphasic separa-tion begins.
Spindle-fibres, phragmoplasts or cell-plates were not seen during division; cytokinesis is accomplished by means of constriction.
Some abnormalities are described.
The haploid chromosome number is seven.

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1. Cytological studies on chromosome number and morphology and embryo sac development have been made on Leucocoryne ixioides in order to compare it with Allium, Nothoscordum, and Brodiaea.
2. Nine haploid chromosomes were found in the pollen grain division: two short with terminal attachments, and seven long with median or sub-median attachments. This configuration resembles those of Nothoscordum and the 9-paired Allium species, but not those of Brodiaea.
3. The macrospore mother cell divides giving rise to two macrospores, one of which degenerates. The other divides three times giving rise to eight nuclei which become arranged in the “normal” way. This behavior is similar to that in Allium and Nothoscordum.
4. The evidence presented points to a closer relationship between Leucocoryne and Allium and Nothoscordum than between the former and Brodiaea.

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1. The events of microsporogenesis in Heuchera americana L., n-7, are presented as characteristic of a group of higher plants whose reduction divisions still retain traces of previous hybridization of the species.
2. Comparison is made between such meiotic irregularities as are found in H. americana and various types of sex chromosomes, to the discredit of the latter.

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1. Der Flavingehalt von 2 Hefearten and 15 Bakterienarten wurde nach der Lumiflavinreaktion von WARBURG und CHRISTIAN bestimmt. Der Gehalt liegt bei Micrococcus ochraceus, Mic. citreus, Bacillus pyocyaneus und Bacterium coli cornmunior in der Höhe von 10-15γ Lumiflavin pro g Trockengewicht and bei alien anderen Bakterien-and Hefearten stets unter 10γ; bei Bac. fuorescens albus und Bac. prodigiosus sogar unter 1.5γ
2. Einige Fehlerquellen, die bei der quantitativen Bestimmung des Flavins in Betracht kommen, d.h. die Zersetzung des Flavins bei der Belichtung, der Verlust des Flavins beim Adsorptions-Elutions-Verfahren sowie die Verschiedenheit zwischen photometrisch and fluorometrisch bestimmten Flavinwerten, wurden erwähnt.
3. Die Rolle des Flavoproteins bei der cyanhemmbaren bzw. cyanresistenten Zellatmung der Mikroorganismen wurde kurz diskutiert.
Zum Schluß möchten wir gern Herrn Prof. Hon. Dr. K. SHIBATA, Herrn Prof. Dr. H. HATTORI sowie Herrn Prof. Dr. T. SAKAMURA für liebenswürdige Unterstützung bei dieser Arbeit unseren besten Dank aussagen.

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1. A basal body is always found at the base of flagellum in the flagellated plants and planocytes studied.
2. In most of the flagellated cells the conjectioplast is seen connecting the basal bodies with nucleus. Accordingly the movement attained by means of the flagellum (or flagella) is thought to be accomplished by cooperative action of the flagellum, basal body, conjectioplast, or rhizoplast and nucleus.
3. The structure of the motor-apparatus is specific to the flagellated cell of every species studied.

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1. Revised chromosome maps of the X-chromosome of D. ananassae are published (Fig. 1).
2. By using two kinds of inversions which are present in the X-chromosome, it has been concluded that a part of the X-chromosome is homologous to a part of the fourth chromosome.
3. From the data on the X-chromosome of D. ananassae, it is as-sumed that high temperature does not affect on the frequency of crossing-over in a heterochromatic portion.
4. The CIIL-O Inversion shown in my previous paper belongs to the racial inversion distributed commonly in wild populations.
5. Some apparently male intersexes due to gene action were described.

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Duch Lebendbeobachtung der Staubfadenharrzellen von Tradescantia reflexa wurden die Einwirkungen verschiedener flüchtigen Substanzen und einiger Gase auf die Teilungsfiguren untersucht and die reversiblen Veränderungen der Zellen in der Teilung werden in 4 Gruppen geteilt und erklärt. Die Ursachen für das Zustandekommen von Teilungsanomalien bei den Tradescantia-Haarzellen wurden auch erörtert.
Gruppe I enthält die Dämpfe von Chloroform, Kohlenstofftetrachlorid, Methylalkohol, Äthylalkohol, Äther, Anisol, Xylol, Benzin and ferner Leuchtgas. Bei der Behandlung der Teilungs-figuren mit diesen Dümpfen ist die Entmischung des Zytoplasmas ausgeprägt.
Gruppe II enthält die Dämpfe von n-Butylalkohol, Aceton, Formalin, Kohlenstoffbisulfid und Benzol. Bei der Behandlung mit diesen Dämpfen tritt zuerst die Verflüssigung und dann die Entmischung des Zytoplasmas auf. Die Dämpfe von n-Butylalkohol, Kohlenstoffbisulfid und Aceton rufen zufällig Teilungsanomalien im lebenden Zustande hervor.
Gruppe III enthält Cyan- und Schwefelwasserstoff und Essigsäuredampf. Diese Dämpfe verursachen die Gelifikation des Zytoplasmas, aber die Entmischung des letzteren ist dabei nicht merklich. Der Dampf von verdünnter Essigsäure bildet abnorme Teilungsfiguren im lebenden Zustande.
Gruppe IV enthält den Ammoniadampf. Der Dampf ruft zuerst die Quellung des Zytoplasmas und der Chromosomen and dann die Entmischung des Zytoplasmas hervor.
Zum Schluß erlaube ich mir, Herrn Hon. Prof. Dr. K. FUJII für die ständige Anregung dieser Untersuchungen meinen herzlichsten Dank auszusprechen. Desgleichen spreche ich hiermit der Japanischen Gesellschaft zur Förderung der zytologischen Förschung für die finanzielle Unterstützung dieser Arbeit meinen besten Dank aus.

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Die H-Ionenkonzentrationen der Narbe und des Staubbeutels bei ein- bzw. zweigeschlechtlichen Blüten wurden untersucht. Der pH-Wert der Narbe erwies sich ausnahmslos kleiner als der von dem Staubbeutel. Diese pH-Erniedrigung an der Narbe war zu Beginn der Blüteperiode am beträchtlichsten. Es ist sehr wahrscheinlich, daß dieser Unterschied der H-Ionenkonzentration zwischen der Narbe und des Staubbeutels eine Bedeutung für die Befruchtung hat, und zwar deshalb, weil die Pollenkeimung auf Rohrzucker-Agar-Boden beim pH-Wert von Narbe derselben Pflanze am günstigsten stattgefunden hat. Der Unterschied der H-Ionenkonzentration zwischen der Narbe and des Staubbeutels steht wahrscheinlich mit dem Sterilitäts- und Unverträglichkeitsprobleme bei Befruchtung in enger Beziehung.

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1. The abnormal course of nuclear division induced by colchicine in the root tip cells of Allium cepa is described.
2. Since the effect of colchicine differs not only in different tissues in the same root, but also even in the same tissues of the same root tip, the root treated with colchicine is of mixoploid nature.
3. Tetraploid plants are produced in both Lycopersicum esculentum and L. pimpinellifolium; the former has high self-sterility, while the latter has high self-fertility. Besides, among the polyploid are usually found some mixoploid plants which are of no practical value from the breeding point of view.
In conclusion, I have great pleasure in recording my gratitude to Hon. Professor K. Fujii, for valuable criticism and guidance. My thanks are also due to Professor T. Nakai, Director of the Botanical Institute, for the facilities given me for studying at the Institute, and to Professor Y. Sinotô for much helpful advice. The expenses for carrying out of this study were partly defrayed out of a grant from the Japan Society for Advancement of Cytology, to which body the writer wishes to express his best thanks.

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1. Der Verfasser hat den Bastard Triticum Timopheevi (n=14)×Agr. elongatum (n=35) gezüchtet und zytologisch untersucht. Seine somatische Chromosomenzahl beträgt, wie zu erwarten war, 49.
2. In der Reduktionsteilung bildet dieser Bastard bis zu 21 Bivalenten and läßt mindestens 7 Chromosomen als Univalenten bestehen.
Da nach KIHARA der Genomengehalt von Triticum Timopheevi A G ist and derjenige von Agr. elongatum (nach WAKAR) A B D X₁ X₂, so ist anzunehmen, daß einerseits die Genome A miteinander konjugieren (allosyndese), anderseits die Genome X₁ and X₂ (autosyndese). Was die übrigen 7 Bivalenten betrifft, so bilden sie sich wohl auf Rechnung der Konjugation der Chromosomen von den Genomen B and G, welche sich so, als miteinander verwandt erweisen.
3. Es ist kaum anzunehmen, daß das Genom G von Triticum Timopheevi dem Genom D verwandt wäre, da Triticum Timopheevi nicht Triticum vulgare, wohl aber Triticum dicoccum ähnlich ist, bei welchem Genom D fehlt und Genom B vorhanden ist.
4. Die Aflinität der Genome B and G dürfte dafür sprechen, daß Genom G durch entwicklurgsgeschichtliche Veränderung aus Genom B entstanden ist.
5. Der beschriebene Weizen-Queqengrasbastard erwies sich als selbststeril.

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1) Die Zellteilung der Closterium-Arten wird durch Lichtabschluß, Sauerstoffentzug und Zuckerzugabe deutlich gesteigert und durch Monojodessigsäure und Natriumfluorid, die ebenfalls spezifische Gärungsgifte sind, sichtlich gehemmt.
2) Zusatz von Cystein beschleunigt die Zellteilung dieser Algen und zwar besonders deutlich, wenn den Algen der Sauerstoff zugeführt wird.
Zum Schluß sei es uns gestattet, Herrn Ehrenprof. Dr. K. SHIBATA, Herrn Prof. Dr. Y. SINOTÔ sowie Herrn Dr. H. TAMIYA, für ihre liebenswürdige Unterstützung und Anregung unseren besten Dank auszusprechen.

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1. The production of diploid males in the parasitic wasp, Habrobracon pectinophorae Watanabe was studied by using two recessive mutant characters, white and curved.
2. The frequency varied according to the hereditary factor involved. Higher frequency was obtained in the case when white was used than in other cases.
3. The fertility of diploid males is very high in this species as compared with the American species, H. juglandis. Especially high fertility was found in the case of curved. Therefore, the fertility of diploid males also depends on the hereditary factor involved.
4. The daughters of the diploid males laid many eggs which, however, failed to hatch, though embryos were formed within.
5. The chromosome complexes of all the types of males and females were studied. The diploidy of biparental males and triploidy of their daughters have been confirmed definitely. In the triploid females, haploid, diploid and triploid chromosomes seem to be mixed in the oögonial cells.
6. A gynandromorph with diploid male parts and a haplo-diploid mosaic male are described.
I wish to express my heartfelt gratitude to Prof. Dr. T. Komai for his suggestions and kind guidance during the course of this work. I am also indebted to Messrs. T. Hama, K. lyatomi, J. Sonan and M. Nakayama for their kindness in supplying me with the material.

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1). The maturation divisions of Scirpus lacustris L. var. typicus Honda f. pictus Honda (2n=39s+1S) and of S. lacustris L. var. Tabernacmontani Trautv. f. zebrinus Makino (2n=42s) have been described. The first plant pictus having one large compound chromosome in a somatic cell, has shown a sort of heteromorphic pairing in the first meiotic division as expected. The last form zebrinus has shown rather regular meiosis.
2). In the 1-metaphase of pictus a number of chromosomal configurations have been observed (cf. tables 1, 2); namely, in the first group (i) the compound chromosome S has conjugated with 3 small chromosomes (3s), giving a formula (1S+3s)+(15+n)_II+(6-2n)_I where n=0-3; in the second group (ii) S has conjugated with 2 small chromosomes (2s), giving a formula (1S+2s)+(15+n)_II+(7-2n)_I where n=0-3; (iii) in the third group, S has conjugated with only one Small chromosome (s), (1S+1s)+(14+n)_II+(10-2n)_I where n=0-4; (iv) in the fourth group, S has failed to conjugate with any small chromosomes, (1S+0)+(17+n)_II+(5-2n)_I where n=0-2; (v) in the fifth, several abnormal Gases were also observed. In Gase of zebrinus, the first division being rather regular, the chromosome counts in the I-metaphase were made easily; 168 PMC's (84%) out of 200 observed were consisted of 21 bivalents.
3). The compound multivalents formed in the first division of pictus have shown various mode of segregation in the I-anaphase. This hetermorphic pairs have segregated equationally as well as reductionally, according to the number of chiasmata between the compound chromosome and the univalents attached to it. Lagging chromosomes were observed now and then. The compound multivalents have always separated slowly. This is probably caused by the chiasma formation.
4). Chromosome counts in the II-metaphase were difficult. However, a few of them obtained have given some hints to the behaviour of the univalents and of the heteromorphic pairing. Judging from the tables 3 and 7, the univalents observed in pietus may sometimes divide themselves in the I-metaphase, and sometimes pass to the poles at random as it is in fast.
5). In the II-anaphase of pictus, remarkable structural hybridity, i.e. formation of chromatin bridges, occurrence of the lagging or precession chromosomes were observed. Chromatin bridges have been considered to have produced from the results of both crossing-over and unusual crossing-over between the compound chromosome and 1-3 small chromosomes attached to the formen. Generally, chromatin bridges have been formed in the first division and rarely in the second. But in the present Gase, the conditions were entirely reversed, i.e. they were not observed in the first division but only in the second.
6). In the II-telophase of pictus, chromatin bridges were persistent. However laggards should have reached to the polen in time to he included, for the percentage of the cells containing laggards had decreased as the stage proceeds.
7). As a result of irregularities during the meiosis, there must arise various pollen grains containing variated chromosomal constitutions. Unfortunately, the pollen grain division was observed only in zebrinus, where the chromosome numbers counted have varied 18 to 22.
8). Test of goodness of pollen grains of 3 types, i.e. typicus (normal self-colored), pictus (variegated form), and zebrinus (variegated form), has shown 99.81% good for typicus, 0% for pictus, and 37.6% good for zebrinus.
9). From the present karyological observations it has been shown that the large chromosome in pictus is equivalent to 3 small chromosomes which were usually non-homologous to each other.

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1. Cytokinesis is considered in two phases, 1) the bipartition of the cytoplasm of the mother cell by the development of phragmoplast, and 2) the MLI formation by which the bipartition of the entire cell-body is effected.
2. The processes whereby the remains of the phragmoplast is dissolved in the cytoplasm are described.
3. The substance of the remains of the phragmoplast is not transformed directly into the plasma membrane. The plasma mem-brane lining the MLI is that of the cytoplasm which migrated through the remains of the phragmoplast toward the MLI, and spread over its surface.
4. In the cytokinesis of PMC the plasma furrow is preceeded as a rule by the MLI formation otherwise by the complete phragmoplast formation across the equator.
5. So-called wall protrusion of the inner lamella of the PMC is the result of the secondary and tertiary callose accumulation in the ist and 2nd cell divisions of PMC, produced after the completion of MLI, namely after the bipartition of the mother protoplast so that the wall protrusion cannot be the cause which leads to the cytokinesis of the PMC.
Here let me express my hearty thanks to Prof. K. Fu ir who has encouraged me throughout the course of this study, and has given me much advice.

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