Clusters of acinar cells were isolated by collagenase digestion of submandibular glands of female BALB/c mice (Isolated Acinar Cells; IAC), and cultured in either serum-supplemented or serum-free medium. The cells grown in the serum-free medium with low Ca
2+ concentration were identified as ductal origin from their histochemical characteristics (Cultured Ductal Cells; CDC).
Kinetics of
36Cl
- and
99mTcO
4- uptake by IAC and CDC was studied by the measurement of the radioactivity. Furosemide and 4, 4-diisothiocyano-2, 2-stilbene disulfonic acid (DIDS) were used for inhibitors of a Na
+/K
+/Cl
- cotransporter and a Cl
-/HCO
3- antiporter, respectively.
The following results were obtained:
1) In both IAC and CDC,
36Cl
- uptake increased rapidly within 5min. and then reached a steady-state, while
99mTcO
4- uptake showed a similar pattern to the initial phase of
36Cl
- uptake but continued to increase slowly thereafter.
2)
36Cl
- uptake in IAC was reduced to about 40% by furosemide or DIDS, and completely blocked by a simultaneous exposure to both inhibitors. In CDC, it was reduced to 18% and 27% by furosemide and DIDS respectively, and mostly blocked by a simultaneous exposure to both.
3)
99mTcO
4- uptake in IAC was reduced to 42% and 69% by furosemide and DIDS respectively, and reduced to 26% by a simultaneous exposure to both inhibitors. In CDC, it was reduced to 7% and 70% by furosemide and DIDS respectively, and mostly blocked by a simultaneous exposure to both.
4) Efflux of
99mTcO
4- from both IAC and CDC was increased by exposure to autonomic nervous agents.
It was concluded from these results that
99mTcO
4- might accumulate in both IAC and CDC via a Na
+/K
+/Cl
- cotransporter and a Cl
-/HCO
3- antiporter. Otherwise, it was suggested that additional mechanism might exist in acinar cells.
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