The Sysmex pocH-100iV (pocH-100iV ) performance characteristics were evaluated based upon analysis of samples taken from healthy dogs and cats. When studies for linearity were performed, the low-end linearity for the samples tested was good for white blood cells (WBC), red blood cells (RBC), hemoglobin (HGB), hematocrit (HCT) and platelet (PLT) measurements. The high-end linearity results also showed good performance for RBC, HGB and HCT. Reproducibility studies were performed simultaneously with the linearity studies and the results showed good performance. When the pocH-100iV was compared with other methods, including an automated hematology analyzer (CELL-DYN 3500), the microcentrifuge hematocrit method and the manual method for PLT, the results agreed well for all measurements except the mean corpuscular hemoglobin concentration (MCHC). To assess the performance of the pocH-100iV in the presence of hematological abnormalities, 54 samples from dogs with hematological abnormality were analyzed and compared with the other methods. The results indicated that the pocH-100iV provided accurate results relating to the hematological abnormalities present in each sample. In conclusion, the high reproducibility and good error detection results suggest that the performance characteristics of the pocH-100iV might be equal to that of other conventional automated instrumentation and methods and reliable to use in a veterinary clinic setting.
The medical records of 137 rabbits with molar malocclusion and 345 controls were investigated retrospectively for epidemiologic research on molar malocclusion risk factors in rabbits. We also asked some of the pet owners to fill in a questionnaire on the contents of the rabbit's daily diet, getting answers about 34 patient animals and 58 controls. The mean weight of the patient group was significantly lower than that of the control group, which may indicate that smaller rabbits tend to suffer from molar malocclusion. The morbidity of this dental condition was higher in mixed-breeds, whereas it was lower in lop-eared breeds. Male rabbits showed significantly higher morbidity than fcmales. The mean amount of hay intake in the patient group was significantly smaller than that in the control group. This may confirm other reports that less hay intake is one of the risk factors of molar malocclusion. In our point of vicw from these data, owners should be instructed to feed their rabbits large amounts of hay under an appropriate feeding program. Especially rabbits with potential risk factors such as being mixed-breed, male, and/or small should be fed more carefully. Such precautions can be effective in preventing incidence and progress of molar malocclusion.
A dog with primary hyperparathyroidism was successfully treated by medical management and surgical resection of the affected parathyroid gland. A female shih tzu was suffering from anorexia, vomiting, polyuria, and polyposia. Blood tests showed hypercalcemia although the parathyriod was intact. Based on these findings, the diagnosis of hyperparathyroidism was established. At the owner's request, medical treatment was chosen. Pamidronate disodium and alendronate sodium were administered, and the dog's quality of life was improved. However, when stable remission could no longer be hoped for, the parathyroid gland was removed surgically. After excision of the parathyroid gland along with the ipsilateral thyroid gland, histopathologic examination of the removed parathroid gland showed hyperplastic change which seemed to be benign. The dog has been in good condition for 19 months since then.
We report a case of feline spontaneous hyperadrenocorticism, which is rare in cats. The disease is often accompanied by noninsulin dependent diabetes mellitus. A 10-year-old female mixedbreed cat was referred to our hospital with main complaints of systemic depilation, depression, and the loss of appetite. Emaciation and an enlarged abdomen with thinned abdominal skin was also observed. The owner reported that up to a few days previous, polidipsia, polyuria, and hyperorexia had been noted. Based on the results of blood tests, a low-dosage dexamethasone suppresion test, and no past history of steroid medication,the cat was diagnosed as having spontaneous hyperadrenocorticism with diabetes. Administration of metyrapone slightly increased the cat's appetite. However, urinary sugar levels remained abnormally high, and the cat died 14 days after the initial treatment. Histopathological examination revealed adrenal hyperplasia.
A five-year-old male Yorkshire terrier weighing 3.2 kg was referred because of chronic small intestine diarrhea, anorexia, vomiting, and abdominal dilatation. Abdominal ultrasonography showed ascites, thickening of the small intestinal wall, and hypermotility of the small intestine. For further characterization of the ultrasonographic findings, flexible endoscopy was performed, which revealed multiple whitish spots like grains of rice in the duedenal mucosa. Several biopsy specimens obtained from different spots were examined histopathologically, and a diagnosis of intestinal lymphangiectasia was made. Generally, endoscopy is less invasive than surgical procedures, which is favorable for treating animals in unstable condition. In this case, endoscopy was useful in diagnosing the dog's disease. We also learned that endoscopy in combination with ultrasonography could enhance the accuracy of the diagnosis.
The clinical value of a canine blood type grouping kit was evaluated. This type of kit is designed to check erythrocyte agglutination with DEA (Dog Erythrocyte antibody) 1.1 monoclonal antibodies. In 79 hematologically normal dogs, 57 were determined to be DEA1.1 positive (72.2%), and the remaining 22 dogs were determined to be DEA1.1 negative. Eighteen of the DEA1.1 positive dogs and 6 negative dogs were selected at random, and the same sample from each animal was tested five times, and showed excellent reproducibility. Ten DEA1.1 positive and 10 negative blood samples from randomly selected dogs were matched by a conventional method, cross matching with one sample of DEA1.1 positive blood. There was no agglutination seen in any case, which confirmed that it is impossible to distinguish DEA1.1 positive and negative by crossmatching. From these findings, it is concluded that this kind of canine blood typing kit is useful in judging DEA1.1 types easity and rapidly. In clinics, the kit would be of much use as a compatibility test before transfusinon.