Urinary enzyme levels after renal artery shutdown were examined in 18 adult, mixed-breed, 6 to 21 kg dogs, which were divided into three groups: 60-min shutdown, 90-min shutdown, and a control. While serum creatinine and blood urea nitrogen levels did not change in the 60-min shutdown or the control group, they were significantly elevated (P<0.05) in the 90-min group 40 hours after the shutdown. Creatinine clearance showed no change in the control, but in the 60-min and 90-min groups it decreased significantly (P<0.05) after one to two hours. Enzymes in the urine, on the other hand, all increased significantly (P<0.05) in the 60-and 90-min groups after one to two hours, then recovered to the normal ranges. From these findings, urinary enzymatic activities may be useful for detection of renal failure in the early stage.
A ten-year-old male beagle was referred to our hospital with complaints of severe coughing and multiple ventricular extrasystole. The dog was given supportive medication, but died 18 days after the first visit to us. Autopsy revealed a huge primary lung tumor, which was histologically diagnosed as a moderately differentiated squamous cell carcinoma with formation of cancer nodules. The neoplasms showed alveolar structure of neoplastic spinous cells with atypical nuclei, numerous mitoses, and single cell keratinizatio Immunohistochemically, the neoplastic cells reacted strongly to cytokeratin antibody, and most of the cytoplasm reacted to p53 protein antibody. Electron microscopy revealed a small number of tonofilaments within the neoplastic cells, and a large number of desmosomes among the neoplastic cells. This is the first report of the presence of p53 protein in cytoplasm in canine pulmonary squamous cell carcinoma.
Eight rabbits with corneal occlusion syndrome treated by surgical excision of the conjuctivae were surveyed retrospectively regarding their age, sex, clinical findings, and post-operative course. The period when the clinical signs were initially noted ranged from 4 to 14months of age. There were as many males as females. Before the surgery, the conjunctivae had overgrown to cover the cornea. Such aberrant conjunctival overgrowth was seen on both eyes of all the rabbits except for two rabbits with trouble in only one eye. Eye discharge and/or redness were also seen in some cases. Judging that narrowed visual fields were disturbing the rabbits'eyesight, we removed the overgrown conjunctivae surgically under general anesthesia. Out of fourteen eyes with the visual problems, five eyes recovered, and four eyes were obviously improved. However, the remaining five needed further surgery. Relapse tended to occur due to insufficient removal of the conjunctiva, or partial adherence of the conjunctiva to the cornea. Histopathologically, intranuclear inclusion of paoilloma virus was found in two cases.
A 5-year-old male dachshund with a gait disorder was brought into the emergency room 3 hours after head trauma. Blood tests did not show any marked abnormalities. A lateral skull radiograph showed a 4 cm fissured fracture running from the posterior edge of the right frontal sinus to the right temporal bone. Despite conservative therapy, the physical condition of the dog did not improve remarkably, and the dog became comatose during 9 hours of observation, and became tetraplegia. A CT scan revealed a 4×4 cm depressed fracture and fissure on the cranial bone from the right frontal sinus to the right temporal bone, accompanied by a 2×3×3.6 cm convex high density area adjacent to the skull fracture. Immediately after the diagnosis of an acute epidural hemorrhage based on typical CT findings, a craniotomy was performed, and the hematoma was reduced. Consequently, the symptoms were ameliorated.
The anthelmintic efficacy of benzimidazoles (parbendazole, thiabendazole, and albendazole) and ivermectin were evaluated in this study. They were given orally to eight dogs with Strongyloides stercoralis as the only parasite. When one medicine turned out to be ineffective in one dog, a second medicine was tried in the same dog. Eventually the number of dogs reached fourteen. First, parbendazole was given to two dogs at 30 mg/kg body weight once a day for 3 days, but showed no efficacy agaisnt Strongyloides stercoralis. Thiabendazole, which was given to five dogs at 75 mg/kg B.W. once a day for 3 days, was effective in three dogs (60% extermination). Albendazole, which was given to five dogs at 25 mg/kg B.W. twice a day for two days, showed 60% efficacy. Ivermectin, which was given to two dogs at 200 μg/kg B.W. one time, showed 100% efficacy.
An assessment of the clinical value of a feline blood-typing kit which utilizes erythrocyte agglutination by anti-A monoclonal antibodies and Triticum vulgaris lectin is reported. The kit was tested in 65 cats without hematological diseases. Sixty cats were judged to have type A blood (92.3%), two to have type B (3.1%), and the remaining three to have type AB (4.6%). Six cats with type A blood, two with type B, and two with type AB were selected and the same sample from each animal was tested five times. All the results showed excellent reproducibility. Seven samples of type A blood, one of B blood, and two of AB blood were conventionally crossmatached. RBCs from A blood and AB blood always agglutinated with plasma from type B blood. But in some cases, RBCs from B blood and AB blood did not agglutinated with plasma from A blood. These findings show that type A blood without naturally acquired anti-B antibodies can not be distinguished from type AB blood by crossmatching. It is concluded that this kind of feline blood typing kit is useful in judging blood types of the AB system easily, rapidly, and more correctly than crossmataching. In other words, it could be used in clinics not only as a compatibility test before transfusion but as a blood typing agent for mating to avoid neonatal isoerythrolysis.