This study aims to investigate HER2/neu protein expression in feline mammary tumors by using human polyclonal antibodies. It has been reported that HER2/neu protein expression is present in about 15−20% of the mammary gland tumors in humans and is thought to be related to malignancy and poor prognosis. Winston et al. investigated HER2/neu protein expression in feline mammary tumors in 2005 and found that its expression was observable in 90% of the (27 out of 30) felines by using human polyclonal antibodies and 76.7% of the (23 out of 30) felines using monoclonal antibodies. Thus, we proposed the following hypothesis: since HER2/neu protein expression in felines was identified using human polyclonal antibodies, we might be able to conduct clinical trials of HER2 inhibitors, which are molecular target drugs; and re-investigated HER2/neu protein expression using human polyclonal antibodies. Our targets were 25 tumors from 13 felines undergoing radical unilateral mastectomy during the period from September 2013 to October 2015. We evaluated HER2/neu protein expression using human polyclonal antibodies and immunohistochemical staining. HER2/neu protein expression was observed only in one out of 13 cases (7.7%). These results were significantly different from the results reported by Winston et al. using human polyclonal antibodies, which showed a higher detection rate. Therefore, further study of the HER2/neu protein expression is required.
The connection between mental and physical health is becoming increasingly recognized in the veterinary field. Many people have come to recognize that socialization during the puppy period is particularly important. Hosting puppy classes in veterinary clinics is not only a way to provide an opportunity to the puppies to socialize; it is also useful for exposing them to various stimuli in a nonthreatening way, teaching proper manners as expected by society, and allowing owners to begin a puppy health care program. Despite these benefits, hosting puppy classes in veterinary clinics remains uncommon. In order to learn more about puppy classes in veterinary clinics, we created questionnaires for clinics that had already held puppy classes and those that had not. Most clinics that held puppy classes said, “it brings numerous benefits to clinics," while those that had not held them thought that it causes more harm than good. Our results show the benefits, and therefore the necessity, of hosting puppy classes in veterinary clinics.
Physical and radiographic examinations of a spayed 14-year-oid toy-poodle, which had lameness in a forelimb after a fall, led to a diagnosis of avulsion of the triceps tendon insertion. A modified 3-loop pulley suture pattern was used for a reduction surgery at an early stage of the injury, and postoperative recovery was favorable. In contrast to previously reported chronic cases, contracture of the triceps and fibrosis of the stump were not observed. Thus, some differences were identified, and recovery time was shorter in this case. Therefore, early diagnosis and treatment are considered important in a case of avulsion of the triceps tendon insertion.