Environment Control in Biology
Online ISSN : 2185-1018
Print ISSN : 0582-4087
ISSN-L : 0582-4087
Volume 34, Issue 2
Displaying 1-9 of 9 articles from this issue
  • Akihiro NOSE
    1996 Volume 34 Issue 2 Pages 97-104
    Published: June 30, 1996
    Released on J-STAGE: June 22, 2010
    JOURNAL FREE ACCESS
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  • Toyoki KOZAI, Chieri KUBOTA, Yoshiaki KITAYA
    1996 Volume 34 Issue 2 Pages 105-114
    Published: June 30, 1996
    Released on J-STAGE: June 22, 2010
    JOURNAL FREE ACCESS
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  • C.Kamande NDUNG'U, Masumi SHIMIZU, Goro OKAMOTO, Ken HIRANO
    1996 Volume 34 Issue 2 Pages 115-122
    Published: June 30, 1996
    Released on J-STAGE: June 22, 2010
    JOURNAL FREE ACCESS
    Young “Riesling” grapevines were induced to produce second shoots by water stress before summer pruning. The vines, field-grown and established in isolated soil beds were subjected to water stress by disconnecting irrigation for 15 and 23 days. Water stress enhanced budbreak and cluster formation. In the early stage of water stress, abscisic acid (ABA) content increased in the canes and trunks, while it decreased in the roots. As the stress persisted, ABA content dropped in the canes and trunks, but increased in the roots. The levels of nitrogen, amino acids, and soluble sugars in the canes, trunks, and roots increased during water stress, while the levels of starch decreased. The results were discussed in relation to interactions of ABA with metabolism of amino acids and sugars. A high level of ABA during water stress was proposed to play a role in stimulating nutrient accumulation. A high reserve of nutrients, being available upon resumption of watering, was proposed to stimulate budbreak and subsequently to improve flower formation.
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  • —Automatization of Evaluating Spray Formation Including Leaves through Shape Features Analysis—
    Kazuhiro KAI, Naoshi KONDO, Takahiro HAYASHI, Yasunori SHIBANO, Kuniyo ...
    1996 Volume 34 Issue 2 Pages 123-128
    Published: June 30, 1996
    Released on J-STAGE: June 22, 2010
    JOURNAL FREE ACCESS
    In the previous papers of this series, the spray formation of cut chrysanthemum by the use of image processing and neural network was evaluated and the usefulness of the method was reported. However, cut chrysanthemums used were without leaves. In this paper, the automatic evaluation system for spray type chrysanthemum with leaves was examined. First, the spray formation of the chrysanthemum was expressed by polygonal approximation in the procedure of image processing. Secondly, the evaluation indexes were calculated based on the polygon. Thirdly, theevaluation indexes were inputted to neural networks and some cut chrysanthemums were evaluated. The following results were obtained : 1. Binary images of whole chrysanthemum and inflorescence were appropriately extracted from the original image through two threshold levels calculated by the gray level histogram. From the binary image, position of the bottom node of the chrysanthemum was precisely detected. 2. The shapes of the spray formations were approximated by polygons in the binary images. 3. The three evaluation indexes, which were related to the approximate shape of the cut chrysanthemum and the degree of dispersion of inflorescence, were calculated based on the polygons. 4. The evaluations by neural networks with three indexes corresponded to those by experts.
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  • Masaaki HOHJO, Tadashi ITO, Shoko TANAKA
    1996 Volume 34 Issue 2 Pages 129-134
    Published: June 30, 1996
    Released on J-STAGE: June 22, 2010
    JOURNAL FREE ACCESS
    This study was conducted to investigate the effect of changing the nutrient concentration at different plant growth stages on growth, yield, fruit quality and physiological characters of tomatoes (Lycopersicon esculentum Mill.) grown hydroponically. Nutrient concentrations adopted were ; a) 1.2 (L), b) 1.8 (M) and c) 3.0 mS/cm (H) . Growth stages when the treatments of nutrient concentration started were, a) florescence, b) fruit development and c) harvesting of the first truss respectively. Treatment combinations were ; LMH ; LMM ; MMM ; HMM ; and HHH. The results are as follows ; No significant difference was observed in the plant fresh weight among treatments. Higher rates of photosynthesis and respiration were recorded in the plants of lower nutrient concentrations. Low uptakes of water and Ca were observed in the plant of higher nutrient concentrations. The occurrence of blossom-end rot was the highest when nutrient concentrations were kept at high levels. Fruit sugar content and titratable acidity were relatively higher in the plants grown under higher nutrient concentrations. These results indicated that changing the nutrient concentration over a wide range affected clearer influences on yield, fruit quality and plant physiology, than those grown without changing the nutrient concentration in tomato plants grown hydroponically.
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  • Toyoki KOZAI, Chieri KUBOTA, Kosuke SAKAMI, Kazuhiro FUJIWARA, Yoshiak ...
    1996 Volume 34 Issue 2 Pages 135-139
    Published: June 30, 1996
    Released on J-STAGE: June 22, 2010
    JOURNAL FREE ACCESS
    Eggplant (Solanum melongena L.) plug seedlings were stored for 3 weeks at 9°C air temperature and photosynthetic photon flux density (PPFD) of 0 (darkness), 2, 8, or 16μmol m-2s-1. The seedlings stored for 3 weeks in darkness or under 2μmol m-2s-1 PPFD reduced dry weight, while those under 8 or 16μmol m-2s-1 PPFD increased dry weight. The light compensation point of seedlings before storage was 8μmol m-2s-1 PPFD and it decreased to 4-5ymol m-2s-1 PPFD after storage regardless of PPFD in storage. All the plantlets stored under light survived at transplanting and grew successfully after storage. Dark storage lowered the photosynthetic capacity and, thus, lowered percent survival at transplanting and post-storage growth rate. For keeping seedlings at no-growth status and preventing quality degradation, it would be required to store the seedlings under conditions where they have null CO2 exchange rates throughout the storage period.
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  • Takashi IKEDA, Hiroshi NONAMI, Yasushi HASHIMOTO
    1996 Volume 34 Issue 2 Pages 141-146
    Published: June 30, 1996
    Released on J-STAGE: June 22, 2010
    JOURNAL FREE ACCESS
    Mechanisms of cell elongation were studied by using soybean (Glycine max [L.] Merr.) embryos subjected to environmental stresses under tissue-culture conditions. Water potential of culture media ranged from -0.02 to -0.80 MPa so that nutrient deficiency and salt stress conditions could be applied to plants grown in the media. According to Lockhart's growth equation, cell expansion is controlled by hydraulic conductance, growth-induced water potential, wall extensibility and effective turgor. All parameters were measured in elongating stems and roots of soybean embryos by using the isopiestic psychrometer and pressure probe. Wall extensibility was significantly larger than hydraulic conductance, and the effective turgor was significantly smaller than the size of the growth-induced water potential. Therefore, we conclude that cell elongation rates were primarily regulated by how much water could be absorbed by elongating cells and the size of the water potential difference between elongating cells and the water source under environmental stresses.
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  • Miyoshi HAKOZAKI, Yuko YOSHIDA, Mitsuteru SUZUKI
    1996 Volume 34 Issue 2 Pages 147-151
    Published: June 30, 1996
    Released on J-STAGE: June 22, 2010
    JOURNAL FREE ACCESS
    Seeds obtained from kiwifruit (Actinidia deliciosa (A. chev.) C. F. Liang et A. R. Ferguson var. deliciosa cv. Hayward) were germinated and the calli derived from the hypocotyl of the seedlings were used to test various methods of cryopreservation. Some calli were found to be preserved after immersing in liquid nitrogen (LN) . The effective measures for preserving calli after immersing in LN are as follows ; calli were cultured with 24% (w/v) or 41% (w/v) sucrose for 2 days, and then after being dehydrated twice, first for 22 min with 60% (v/v) PVS2 (30% (w/v) glycerol, 15% (w/v) dimethyl sulfoxide, 15% (w/v) ethylene glycol and 13.7% (w/v) sucrose) and then for 23 min with 100% (v/v) PVS2, were preserved in LN. After cryopreservation, the calli were warmed in 37°C water immediately after being taken from the LN. They were washed in a liquid culture medium containing highly concentrated sucrose and then revived in a medium from which ammonium nitrate (NH4NO3) had been removed. These results will be important to the development of cryopreservation methods of kiwifruit germplasm.
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  • Ryoko KOBAYASHI, Michiko TAKAGAKI, Chieri KUBOTA, Toyoki KOZAI
    1996 Volume 34 Issue 2 Pages 153-155
    Published: June 30, 1996
    Released on J-STAGE: June 22, 2010
    JOURNAL FREE ACCESS
    Radish sprouts (Raphanus sativus L.) harvested in a commercial operation were kept for a day at 20°C air temperature under 0 (darkness) or 50μmol m-2s-1 photosynthetic photon flux density (PPFD) (pre-storage treatment) and stored for 16 days at 5°C air temperature under 0 or 5μmol m-2s-1 PPFD (storage treatment) . The pre-storage treatment was to examine the effect of illumination during shipping conditions. For treatments under light, white light was provided continuously (24 h d-1 photoperiod) with cool white fluorescent lamps. The best quality was obtained under conditions where light was provided throughout the pre-storage and the storage period. Dark storage caused the shoot elongation and chlorophyll degradation. Light in low temperature storage was shown to contribute to keeping high postharvest quality of the radish sprouts.
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