Spirulina alga cultured in a germanium dioxide added pond of Nihon Spirulina Co., Niigata, contained 1, 130ppm of the element in its dried product. A chronic intoxication test on that “
Spirulina” product was conducted by feeding experiment diet to Wistar strain rats for 25 weeks. The rats of both sexes were respectively divided into two groups, and were fed the diet ad libitum. The diet was either 1:4 mixture of the dried “
Spirulina” and the ordinary diet (N. M. F., Oriental Yeast Co., LTD, Tokyo) or the ordinary diet alone. Comparisons were made between the experimental and the control groups in growth and external appearance during the feeding, and in hematological tests and observation of major organs at the end of the experiment. The items of hematological test were number of WBC and RBC, Hb, Ht, MCV, MCH, MCHC, serum protein concentration, A/G ratio, concentrations of urea-N, uric acid, creatinine, total lipids, triglyceride, phospholipids, total cholesterol, HDL cholesterol, total bilirubin, calcium, inorganic phosphate, iron and TIBC, and enzyme activities of ALP, CK, γ-GTP, SGOT, SGPT and LAP. The weight and appearance of organs were observed with regard to adrenal glands, the brain, the heart, hypophysis, the kidneys, the liver, the lungs, the spleen, the stomach and the testes or the ovaries.
There were almost no differences between the experimental and the control groups in the growth and external appearance of the whole body or in the shape, weight and histological findings of the organs. Although hematological tests showed some statistical differences (
p<0.01 by Student's
t-test), e. g. serum calcium and serum iron of the male and triglyceride and CK activity of the female, these values were within the normal range compared to the values of rats in the literatures, and no abnormal findings were seen in the animals in respect of these items. Accordingly, the “
Spirulina” diet used in this experiment was assumed not to bring any toxic effects to rats within the experimental period.
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