Some urinary hormones, namely 3 fractions of Estrogen (Estrone, Estradiol and Estriol) Pregnanediol, 17-Ketosteroid and Chemocorticoid, were simultaneously meausred after the operation through puerperium of a woman who was given the bilateral oophorectomy of ovarian tumor (Dermoid Cyst) at 7th month pregnancy and progressed favorably after operation, delivering at full term. The results are as followings.
1) Estrogenype, quantity and variation degree of excretion of 3 fractions were same as normal pregnancy and no remarkable change was seen due to oophorectomy.
2) Pregnanedioless pregnanediol was observed during 3 weeks after operation in spite of preoperative injection of Proluton Depot 65mg, which was followed by lapid increase of pregnanediol, indicating least quantity of normal pregnancy through the completion of delivery, then remarkable decrease was again recognized.
3) 17-Ketosteroid, Chemocorticoid-17-KS was slightly increased within 1-3days after operation and chemocorticoid of both free and combined forms also increased. They, however, became normal after this period and gradually went down after delivery as usually seen in normal pregnancy.
Although Waldstein and others have already reported concerning invariable excreation of estrogen due to oophorectomy in pregnancy, this is a primary report which measured 3 fractions of estrogen and other hormones at the same time. The fact that no special difference was found in this case means that placenta is very important as a producing organ of these hormones, while ovary is less valued especially during later half of pregnancy when the placental function has been completed, which shows the complicated mechanism of internal secretion in pregnancy.

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1. Alloxan, Dithizon and Oxin were injected to the rabbits which had discharged livers by CCl₄ since ca. 1 month before the injection. Diabetes occured more steadly after the liver discharge than control group, and especially in alloxan and dithizon injected group, permanent diabetes occured in the highest degree.
2. a) The inhibition to the growth of cultured tissues was most manifest in the serum of alloxan injected group, dithizon next, and in the serum of oxin injected group and d.epancreaticomized diabetic dogs, growth inhibitions were more or less slighter.
b) In each group, the growth inhibition was severest at the initial hyperglycemic phase and at the second hypoglycemic phase. At the early period of the permanent hyperglycemic phase, tissue injuries were slightest, and later the injuries became more manifest again.
c) Among the tissues of chick-embryo, liver was most sensitive to the additioned blood-sera of experimental diabetic animals and always exhibited growth inhibition of various severity.
3. When the sera of the experimental diabetic animals inactivated by the heat or filtered by the chamberand filter and or absorbed by caorin, its controllable power were relatively reduced. This, remarkable at the inactivated serum by the heat, but nicht so remarkable at the serum filtered or absorbed.
4. No correlation was found between the severity of the tissue injuries and blood sugar levels of these experimental diabetic animals. Then, as the late Prof. Iisuka discussed, the diabetogenic“toxin”shall exist nicht related to hyperglycemia.

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The present work, as a part of Prof. Iida's study on the sterilization by forming corpus luteum artificially, has been done to estimate the action of trophoblast hormone (T.B.H.) in relation to its luteinizing and luteotrophic effect.
In Part I of this report, the author examined critically the procedures of the quantitative determination of pregnanediol (PG) in urine. In his own work the author adopted with some modification the chromatographic methos of Watteville for extraction and purification of PG in urine and the sulphuric acid method of Guterman with spectrophotometry for its microdetermination. The sulphuric acid method is said to be the most accurate for microdetermination of PG, but it is not the characteristic reaction for PG alone, so the best attention was payed to extraction of PG from urine and its purification. Thus the author succeeded in isolating two samples of crystal with sublimating property from the urine of a pregnant woman. One of them was ketonic (pregnanedion) and the other non-ketonic (PG). Melting points and the result of the organic microanalysis were satisfactorily coincident with the theoretical values for each of them. Making use of this crystal in the sulphuric acid method of Guterman, the relation between the spectral absorption at 420mμ and the quantity (mg) of PG, i.e. the standard curve for the spectrophotometric microdetermination of PG, was decided by means of Beckman's spectrophotometer (type DU). The recovering test and some other tests confirmed the accuracy and the reliability of the method above-described for microdetermination of PG inurine.The author believes that this method may beused for clinical purposes.
In Part II of this report, the author tried to ascertain how the amount of PG in urine varies with the menstruation, the conception and related disorders or diseases. He also tried to elucidate the relation of PG in urine to T.B.H. administration, hypophyseal and suprarenal functions. The results were summarized as follows.
1) In the normal menstrual cycle, PG appeared in urine only at the luteal phase and its apPearance was less marked in the disorderly menstrual cycle. The time of ovulation may be more easily inferable by the appearance of PG in urine than by other methods now in use. When the periods were changed artificially by administration of T.B.H,, PG appeared a little earlier, if administered at the early estrogenic phase, but the T.B.H. administration at the luteal phase caused the elongation of the luteal phase, accompanied by a significant increase in PG excretion.
2) PG in urine refiected truly the ovarian function, particularly the corpus luteum activity, also in the irregular menstrual cycle. The unovulatory cycle were ascertained by PG determination in urine better than by endometrial examination or by B.B.T. test. It was also assured that T.B.H. administration served better in the cure of the irregular menstrual cyle or of the functional sterility than any other hormone therapy, from the view-point of the PG increase in urine.
3) In the earlier stages of the normal conception PG in urine did not show any marked increase as compared with that in the luteal phase, but after the 16th week it remarkably increased and reached its maximum at the 32nd week, which continued till parturition. After parturition PG in urine suddenly decreased and disappeared 98 hours after delivery.
4) The amount of PG in urine was remarkably less in the toxemia of pregnancy as compared with that in the normal conception. This fact was coincident with the results of other clinical examinatlons.Thus it may be presumable that the toxemia of pregnancy is caused by the disorderly renewal of hormones, so the determination of PG in urine may serve as a factor in judging the prognosis of thetoxemla.
5) By administration of T.B.H. for protection from the threatened abortion the amount of PG in urine increased, accompanied by the amelioration of symptoms.

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The effect of the thyroid activity on the antidiuretic potency of blood serum and on the ADH content in neurohypophysis was studied in rats biologically and also by means of Gomori's alum-chrome-hematoxylin-phloxine staining.
In the thyroidectomized rats, 28 days after the operation, the ADH content in neurohypophysis and the blood ADS were slightly higher than normal levels.
In rats injected hypodermically with 0.05 or 0.5ml of Thyradin (corresponded to 10 or 100mg of desicated thyroid) per 100g body weight daily for the periods of 12 or 28 days, ADH content and blood ADS were moderately lower than normal values.
In neither above mentioned cases, any significant cytological change in the posterior pituitary gland and in neurosecretory nuclei of hypothalamus occured.

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